Proteinase K
Parengyodontium album
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain A； UniProt 106–384 | Mutation：S312D | CA CALCIUM ION × 2 NO3 NITRATE ION × 1 | X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 6.5；293 K；0.5 M NaNO3, 0.1 M CaCl2, 0.1 M MES | Resolution 1.60 Å R-free 0.186 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 6K2S | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1BJR COMPLEX FORMED BETWEEN PROTEOLYTICALLY GENERATED LACTOFERRIN FRAGMENT AND PROTEINASE K Deposited 1998-06-27 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICRODIALYSIS；pH 6；279 K；60 MG/ML PROTEIN IN 10MM TRIS.HCL, PH 6.0 WAS MICRODIALYZED AGAINST 10% ETHANOL AT 6 DEGREE CELSIUS, microdialysis, temperature 279K
|
Resolution 2.44 Å R-free 0.225 |
| 1CNM ENHANCEMENT OF CATALYTIC EFFICIENCY OF PROTEINASE K THROUGH EXPOSURE TO ANHYDROUS ORGANIC SOLVENT AT 70 DEGREES CELSIUS Deposited 1999-05-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 CCN ACETONITRILE × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；CRYSTALLIZED BY DISSOLVING THE 10%(W/V) OF LYOPHILIZED ENZYME IN 50MM TRIS.HCL,
1MM CACL2, PH 6.5. 25 MICRO LITRE DROPS OF THIS SOLUTION WERE EQUILIBRATED IN
A MICRODIALYSIS SETUP AGAINST 1M NANO3 IN THE SAME BUFFER AT 4 DEGREE., pH 7.5
|
Resolution 2.20 Å R-free 0.231 |
| 1EGQ ENHANCEMENT OF ENZYME ACTIVITY THROUGH THREE-PHASE PARTITIONING: CRYSTAL STRUCTURE OF A MODIFIED SERINE PROTEINASE AT 1.5 A RESOLUTION Deposited 2000-02-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 ACY ACETIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICRODIALYSIS；pH 6.5；277 K；Sodium Nitrate, Calcium Chloride, Tris-HCl, pH 6.5, MICRODIALYSIS, temperature 277K
|
Resolution 1.55 Å R-free 0.206 |
| 1HT3 MERCURY INDUCED MODIFICATIONS IN THE STEREOCHEMISTRY OF THE ACTIVE SITE THROUGH CYS-73 IN A SERINE PROTEASE: CRYSTAL STRUCTURE OF THE COMPLEX OF A PARTIALLY MODIFIED PROTEINASE K WITH MERCURY AT 1.8 A RESOLUTION Deposited 2000-12-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | HG MERCURY (II) ION × 2 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；sodium nitrate, calcium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.221 |
| 1IC6 STRUCTURE OF A SERINE PROTEASE PROTEINASE K FROM TRITIRACHIUM ALBUM LIMBER AT 0.98 A RESOLUTION Deposited 2001-03-30 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Mutation：S207D | CA CALCIUM ION × 2 NO3 NITRATE ION × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.5；295 K；CaCl2 & NaNO3, pH 6.5, VAPOR DIFFUSION, temperature 295K
|
Resolution 0.98 Å R-free 0.124 |
| 1OYO Regulation of protease activity by melanin: Crystal structure of the complex formed between proteinase K and melanin monomers at 2.0 resolution Deposited 2003-04-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 3ID 3H-INDOLE-5,6-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICRODIALYSIS；pH 8；278 K；50mM Tris-HCl, 0.75M Sodium Nitrate, pH 8.0, MICRODIALYSIS, temperature 278K
|
Resolution 2.02 Å R-free 0.190 |
| 1P7V Structure of a complex formed between Proteinase K and a designed heptapeptide inhibitor Pro-Ala-Pro-Phe-Ala-Ala-Ala at atomic resolution Deposited 2003-05-06 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 NO3 NITRATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
Microgravity with APCF reactors；pH 6.5；295 K；tris HCl, CaCl2, NaNO3, pH 6.5, Microgravity with APCF reactors, temperature 295K
|
Resolution 1.08 Å R-free 0.140 |
| 1P7W Crystal structure of the complex of Proteinase K with a designed heptapeptide inhibitor Pro-Ala-Pro-Phe-Ala-Ser-Ala at atomic resolution Deposited 2003-05-06 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 NO3 NITRATE ION × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
microgravity with APCF reactors；pH 6.5；295 K；Tris HCl, CaCl2, NaNO3, pH 6.5, microgravity with APCF reactors, temperature 295K
|
Resolution 1.02 Å R-free 0.142 |
| 1PEK STRUCTURE OF THE COMPLEX OF PROTEINASE K WITH A SUBSTRATE-ANALOGUE HEXA-PEPTIDE INHIBITOR AT 2.2 ANGSTROMS RESOLUTION Deposited 1993-01-19 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain E
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1PFG Strategy to design inhibitors: Structure of a complex of Proteinase K with a designed octapeptide inhibitor N-Ac-Pro-Ala-Pro-Phe-DAla-Ala-Ala-Ala-NH2 at 2.5A resolution Deposited 2003-05-27 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；289 K；50mM Tris, 1mM CaCl2, 1M NaHNO3, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K
|
Resolution 2.50 Å |
| 1PJ8 Structure of a ternary complex of proteinase K, mercury and a substrate-analogue hexapeptide at 2.2 A resolution Deposited 2003-06-02 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
106–384（279 aa）
|
Not recorded | HG MERCURY (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.5；277 K；20mg/ml HgCl2, 50mM Tris, 1M NaNO3, 10mM CaCl2, pH 6.5, VAPOR DIFFUSION, temperature 277K
|
Resolution 2.20 Å |
| 1PTK STUDIES ON THE INHIBITORY ACTION OF MERCURY UPON PROTEINASE K Deposited 1993-04-07 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | HG MERCURY (II) ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.40 Å |
| 2DP4 Crystal structure of the complex formed between proteinase K and a human lactoferrin fragment at 2.9 A resolution Deposited 2006-05-05 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；0.2M Ammonium actate, 0.1M sodium acetate, 30% PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.90 Å R-free 0.235 |
| 2DQK Crystal structure of the complex of proteinase K with a specific lactoferrin peptide Val-Leu-Leu-His at 1.93 A resolution Deposited 2006-05-29 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；sodium nitrate, CaCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.93 Å R-free 0.187 |
| 2DUJ Crystal structure of the complex formed between proteinase K and a synthetic peptide Leu-Leu-Phe-Asn-Asp at 1.67 A resolution Deposited 2006-07-23 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；sodium nitrate, CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.67 Å R-free 0.192 |
| 2G4V anomalous substructure of proteinase K Deposited 2006-02-22 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 K POTASSIUM ION × 2 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.14 Å R-free 0.203 |
| 2HD4 Crystal structure of proteinase K inhibited by a lactoferrin octapeptide Gly-Asp-Glu-Gln-Gly-Glu-Asn-Lys at 2.15 A resolution Deposited 2006-06-20 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 ACY ACETIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；0.2M Ammonium acetate, 0.1M Sodium acetate hydrate, 30% PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.15 Å R-free 0.202 |
| 2HPZ Crystal structure of proteinase K complex with a synthetic peptide KLKLLVVIRLK at 1.69 A resolution Deposited 2006-07-18 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 NO3 NITRATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；50mM TRIS, 10mM CaCl2, 1M NaNO3, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.69 Å R-free 0.179 |
| 2ID8 Crystal structure of Proteinase K Deposited 2006-09-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 NO3 NITRATE ION × 3 CL CHLORIDE ION × 1 2DB (S)-(2,3-DIHYDROXYPROPOXY)TRIHYDROXYBORATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；50 mg/ml protein, 0.5 M NaNO3, 50 mM citrate buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.27 Å R-free 0.159 |
| 2PKC CRYSTAL STRUCTURE OF CALCIUM-FREE PROTEINASE K AT 1.5 ANGSTROMS RESOLUTION Deposited 1993-06-04 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | NA SODIUM ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.50 Å |
| 2PQ2 Structure of serine proteinase K complex with a highly flexible hydrophobic peptide at 1.8A resolution Deposited 2007-05-01 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
106–384（279 aa）
|
Mutation：S312D | CA CALCIUM ION × 1 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；0.2M AMMONIUM ACTATE, 0.1M SODIUM ACETATE, 30% PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.82 Å R-free 0.207 |
| 2PRK SYNCHROTRON X-RAY DATA COLLECTION AND RESTRAINED LEAST-SQUARES REFINEMENT OF THE CRYSTAL STRUCTURE OF PROTEINASE K AT 1.5 ANGSTROMS RESOLUTION Deposited 1987-11-30 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.50 Å |
| 2PWA Crystal Structure of the complex of Proteinase K with Alanine Boronic acid at 0.83A resolution Deposited 2007-05-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：Proteinase K
|
Not recorded | CA CALCIUM ION × 1 NO3 NITRATE ION × 9 SO4 SULFATE ION × 1 B2A ALANINE BORONIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；CaCl2 & NaNO3,alanine boronic acid, pH 6.5, VAPOR DIFFUSION, temperature 295K, pH 6.50, VAPOR DIFFUSION, HANGING DROP
|
Resolution 0.83 Å R-free 0.136 |
| 2PWB Crystal structure of the complex of proteinase K with coumarin at 1.9 A resolution Deposited 2007-05-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：Proteinase K
|
Not recorded | CA CALCIUM ION × 2 NO3 NITRATE ION × 1 COU COUMARIN × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；CaCl2 & NaNO3, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.90 Å R-free 0.173 |
| 2PYZ Crystal structure of the complex of proteinase K with auramine at 1.8A resolution Deposited 2007-05-17 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：Proteinase K
|
Not recorded | CA CALCIUM ION × 2 NO3 NITRATE ION × 1 AU4 4,4'-(AMINOMETHYLENE)BIS(N,N-DIMETHYLANILINE) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；NaNO3 , pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.79 Å R-free 0.205 |
| 2V8B SAD Structure solution of Proteinase K grown in selenate solution Deposited 2007-08-06 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：RESIDUES 106-384
|
Not recorded | CA CALCIUM ION × 1 SE4 SELENATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；HANGING DROP AT 20C, 50 TO 200 MM NASEO4
|
Resolution 0.94 Å R-free 0.103 |
| 3AJ8 X-ray analysis of Crystal of Proteinase K Obtained from H2O Solution Using PEG 8000 Deposited 2010-05-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；4.5% PEG 8000, 0.05M calcium acetate, 0.05M cacodylate, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.10 Å R-free 0.132 |
| 3AJ9 X-ray analysis of Crystal of Proteinase K Obtained from D2O Solution Using PEG 8000 Deposited 2010-05-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；4.5% PEG 8000, 0.05M calcium acetate, 0.05M cacodylate, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.10 Å R-free 0.162 |
| 3D9Q Proteinase K by LB nanotemplate method before high X-Ray dose on ESRF ID23-1 beamline Deposited 2008-05-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/ml of protein in 25mM HEPES, pH7.0, reservoir solution composed by 25mM HEPES and 400mM Na/K tartrate at pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.43 Å R-free 0.241 |
| 3DDZ Proteinase K by LB nanotemplate method after the first step of high X-Ray dose on ESRF ID23-1 beamline Deposited 2008-06-07 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/ml of protein in 25mM HEPES, pH7.0, reservoir solution composed by 25mM HEPES and 400mM Na/K tartrate at pH7.0. Onto the siliconized glass cover slides were mixed 4 microL of protein solution with 4 microL of reservoir solution., VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.226 |
| 3DE0 Proteinase K by LB nanotemplate method after the second step of high X-Ray dose on ESRF ID23-1 beamline Deposited 2008-06-07 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/ml of protein in 25mM HEPES, pH7.0, reservoir solution composed by 25mM HEPES and 400mM Na/K tartrate at pH7.0. Onto the siliconized glass cover slides were mixed 4 microL of protein solution with 4 microL of reservoir solution., VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.90 Å R-free 0.230 |
| 3DE1 Proteinase K by LB nanotemplate method after the third step of high X-Ray dose on ESRF ID23-1 beamline Deposited 2008-06-07 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/ml of protein in 25mM HEPES, pH7.0, reservoir solution composed by 25mM HEPES and 400mM Na/K tartrate at pH7.0. Onto the siliconized glass cover slides were mixed 4 microL of protein solution with 4 microL of reservoir solution., VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.206 |
| 3DE2 Proteinase K by LB nanotemplate method after the fourth step of high X-Ray dose on ESRF ID23-1 beamline Deposited 2008-06-07 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/ml of protein in 25mM HEPES, pH7.0, reservoir solution composed by 25mM HEPES and 400mM Na/K tartrate at pH7.0. Onto the siliconized glass cover slides were mixed 4 microL of protein solution with 4 microL of reservoir solution., VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.10 Å R-free 0.220 |
| 3DE3 Proteinase K by Classical hanging drop method before high X-Ray dose on ESRF ID23-1 beamline Deposited 2008-06-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/ml of protein in 25mM HEPES, pH7.0, reservoir solution composed by 25mM HEPES and 400mM Na/K tartrate at pH7.0. Onto the siliconized glass cover slides were mixed 4 microL of protein solution with 4 microL of reservoir solution., VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.43 Å R-free 0.229 |
| 3DE4 Proteinase K by Classical hanging drop method after the first step of high X-Ray dose on ESRF ID23-1 beamline Deposited 2008-06-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/ml of protein in 25mM HEPES, pH7.0, reservoir solution composed by 25mM HEPES and 400mM Na/K tartrate at pH7.0. Onto the siliconized glass cover slides were mixed 4 microL of protein solution with 4 microL of reservoir solution., VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.218 |
| 3DE5 roteinase K by Classical hanging drop method after the second step of high X-Ray dose on ESRF ID23-1 beamline Deposited 2008-06-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/ml of protein in 25mM HEPES, pH7.0, reservoir solution composed by 25mM HEPES and 400mM Na/K tartrate at pH7.0. Onto the siliconized glass cover slides were mixed 4 microL of protein solution with 4 microL of reservoir solution., VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.10 Å R-free 0.234 |
| 3DE6 Proteinase K by Classical hanging drop method after the third step of high X-Ray dose on ESRF ID23-1 beamline Deposited 2008-06-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/ml of protein in 25mM HEPES, pH7.0, reservoir solution composed by 25mM HEPES and 400mM Na/K tartrate at pH7.0. Onto the siliconized glass cover slides were mixed 4 microL of protein solution with 4 microL of reservoir solution., VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å R-free 0.234 |
| 3DE7 Proteinase K by Classical hanging drop method after the fourth step of high X-Ray dose on ESRF ID23-1 beamline Deposited 2008-06-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/ml of protein in 25mM HEPES, pH7.0, reservoir solution composed by 25mM HEPES and 400mM Na/K tartrate at pH7.0. Onto the siliconized glass cover slides were mixed 4 microL of protein solution with 4 microL of reservoir solution., VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.30 Å R-free 0.211 |
| 3DVQ Proteinase K by LB nanotemplate method before high X-Ray dose on ESRF ID14-2 beamline Deposited 2008-07-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/ml of protein in 25mM HEPES pH7.0, reservoir solution composed by 25mM HEPES and 400mM Na/K tartrate at pH7.0. Onto the siliconized glass cover slides were mixed 4 microlitres of protein solution with 4 microlitres of reservoir solution., VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.02 Å R-free 0.223 |
| 3DVR Proteinase K by LB nanotemplate method after the first step of high X-Ray dose on ESRF ID14-2 beamline Deposited 2008-07-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/ml of protein in 25mM HEPES pH7.0, reservoir solution composed by 25mM HEPES and 400mM Na/K tartrate at pH7.0. Onto the siliconized glass cover slides were mixed 4 microlitres of protein solution with 4 microlitres of reservoir solution., VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.02 Å R-free 0.216 |
| 3DVS Proteinase K by LB nanotmplate method after the second step of high dose on ESRF ID14-2 beamline Deposited 2008-07-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/ml of protein in 25mM HEPES pH7.0, reservoir solution composed by 25mM HEPES and 400mM Na/K tartrate at pH7.0. Onto the siliconized glass cover slides were mixed 4 microlitres of protein solution with 4 microlitres of reservoir solution., VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.02 Å R-free 0.224 |
| 3DW1 Proteinase K by LB nanotemplate method after the third step high X-Ray dose on ESRF ID14-2 beamline Deposited 2008-07-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/ml of protein in 25mM HEPES pH7.0, reservoir solution composed by 25mM HEPES and 400mM Na/K tartrate at pH7.0. Onto the siliconized glass cover slides were mixed 4 microlitres of protein solution with 4 microlitres of reservoir solution., VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.03 Å R-free 0.221 |
| 3DW3 Proteinase K by Classical hanging drop method before high X Ray dose on ESRF ID 14-2 beamline Deposited 2008-07-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/ml of protein in 25mM HEPES pH7.0, reservoir solution composed by 25mM HEPES and 400mM Na/K tartrate at pH7.0. Onto the siliconized glass cover slides were mixed 4 microlitres of protein solution with 4 microlitres of reservoir solution., VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 0.99 Å R-free 0.235 |
| 3DWE Proteinase K by Classical hanging drop method after high X-Ray dose on ESRF ID14-2 beamline Deposited 2008-07-22 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/ml of protein in 25mM HEPES pH7.0, reservoir solution composed by 25mM HEPES and 400mM Na/K tartrate at pH7.0. Onto the siliconized glass cover slides were mixed 4 microlitres of protein solution with 4 microlitres of reservoir solution., VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 0.99 Å R-free 0.222 |
| 3DYB proteinase K- digalacturonic acid complex Deposited 2008-07-25 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；5% PEG 3350, 0.1 M HEPES; reservoir of 25% PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
Resolution 1.32 Å R-free 0.207 |
| 3GT3 Structure of proteinase K with the mad triangle B3C Deposited 2009-03-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | BRV 5-amino-2,4,6-tribromobenzene-1,3-dicarboxylic acid × 4 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293 K；0.1M TRIS, 1.28M ammonium sulfate, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.50 Å R-free 0.184 |
| 3GT4 Structure of proteinase K with the magic triangle I3C Deposited 2009-03-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | I3C 5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid × 3 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293 K；0.1M TRIS, 1.28M ammonium sulfate, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.76 Å R-free 0.191 |
| 3I2Y Proteinase K by Classical hanging drop Method before high X-Ray dose on ID14-2 Beamline at ESRF Deposited 2009-06-30 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/mL in 25mM HEPES, pH7.0 PMSF, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 0.99 Å R-free 0.215 |
| 3I30 Proteinase K by Classical hanging drop Method after high X-Ray dose on ID14-2 Beamline at ESRF Deposited 2009-06-30 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/mL in 25mM HEPES, pH7.0 PMSF, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 0.99 Å R-free 0.205 |
| 3I34 Proteinase K by LB Nanotemplate Method after high X-Ray dose on ID14-2 Beamline at ESRF Deposited 2009-06-30 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | HG MERCURY (II) ION × 2 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/mL in 25mM HEPES, pH7.0 PMSF, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.00 Å R-free 0.220 |
| 3I37 Proteinase K by LB Nanotemplate Method before high X-Ray dose on ID14-2 Beamline at ESRF Deposited 2009-06-30 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mg/mL in 25mM HEPES, pH7.0 PMSF, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 0.99 Å R-free 0.208 |
| 3L1K SAD structure solution of proteinase K grown in potassium tellurate solution Deposited 2009-12-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：residues 106-384
|
Not recorded | CA CALCIUM ION × 1 TE6 Orthotelluric acid × 1 SO4 SULFATE ION × 2 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；Drop was 1 microL of protein solution (80 mg/mL in dH2O) and 1 microL of well solution (1 mL of saturated solution of K2TeO4. The cryo-solution consisted of 70% of the crystallization solution and 30% of ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.55 Å R-free 0.149 |
| 3OSZ Crystal Structure of the complex of proteinase K with an antimicrobial nonapeptide, at 2.26 A resolution Deposited 2010-09-10 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；tris HCl, CaCl2, NaNO3, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.26 Å R-free 0.193 |
| 3PRK INHIBITION OF PROTEINASE K BY METHOXYSUCCINYL-ALA-ALA-PRO-ALA-CHLOROMETHYL KETONE. AN X-RAY STUDY AT 2.2-ANGSTROMS RESOLUTION Deposited 1991-08-07 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 3PTL Crystal structure of proteinase K inhibited by a lactoferrin nonapeptide, Lys-Gly-Glu-Ala-Asp-Ala-Leu-Ser-Leu-Asp at 1.3 A resolution. Deposited 2010-12-03 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；tris HCl, CaCl2, NaNO3, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.30 Å R-free 0.250 |
| 3Q40 Sulphur SAD structure solution of proteinase K grown in SO4-less solution. Deposited 2010-12-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：residues 106-384
|
Not recorded | CA CALCIUM ION × 2 NO3 NITRATE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；1 uL of 40 mg/ml protein and 1 uL of well solution: 10 mM CaCl2, 500 mM NaNO3, 100 mM Na cacodylate pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.183 |
| 3Q5G Sulphur SAD structure solution of proteinase K grown in SO4 solution Deposited 2010-12-28 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：residues 106-384
|
Not recorded | SO4 SULFATE ION × 1 CA CALCIUM ION × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；1 muL of 40 mg/ml protein mixed with 1 muL of the mother liquor.
Mother liquor : 400 mM Ammonium SO4, 25 % glycerol, 100 mM Na cacodylate pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.77 Å R-free 0.167 |
| 3QMP Selenium SAD structure solution of proteinase K grown in SO4-less solution and soaked in selenate. Deposited 2011-02-04 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 4 GOL GLYCEROL × 1 SE4 SELENATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；UL OF 40 MG/ML PROTEIN AND 1 UL OF WELL SOLUTION: 10 MM CACL2, 500 MM NANO3, 100 MM NA CACODYLATE. Crystal soaked in 33 % (V/V) glycerol, 0.33 M NaSeO4 and 33 % (V/V) mother liquor., pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.10 Å R-free 0.119 |
| 4B5L The 1.6 A High Energy Room Temperature Structure of Proteinase K at 38.4 keV and 0.04 MGy Deposited 2012-08-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；1 UL OF 40 MG/ML PROTEIN IN DH2O MIXED WITH 1 MUL OF THE MOTHER LIQUOR. MOTHER LIQUOR 400 MM AMMONIUM SO4, 25% GLYCEROL, 100 MM NA CACODYLATE PH 6.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 1.60 Å R-free 0.184 |
| 4DJ5 Proteinase K by Langmuir-Blodgett Hanging Drop Method at 1.8A resolution for Unique Water Distribution Deposited 2012-02-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.80 Å R-free 0.202 |
| 4FON High Energy Remote SAD structure solution of Proteinase K from the 37.8 keV Tellurium K edge Deposited 2012-06-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | CA CALCIUM ION × 1 TE TELLURIUM × 1 O OXYGEN ATOM × 5 EDO 1,2-ETHANEDIOL × 2 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；1 uL 60 mg/mL protein + 1 uL reservoir (1 mL saturated potassium tellurate), cryo-solution: 70%
crystallization solution, 30% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.05 Å R-free 0.131 |
| 4WOB Proteinase-K Pre-Surface Acoustic Wave Deposited 2014-10-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；0.6 - 1.6 M Ammonium Sulfate, 0.1 M Tris, pH 8.0
|
Resolution 1.90 Å R-free 0.257 |
| 4WOC Proteinase-K Post-Surface Acoustic Waves Deposited 2014-10-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；0.6 - 1.6 Ammonium Sulfate, 0.1 M Tris, pH 8.0
|
Resolution 1.60 Å R-free 0.207 |
| 4ZAR Crystal Structure of Proteinase K from Engyodontium albuminhibited by METHOXYSUCCINYL-ALA-ALA-PRO-PHE-CHLOROMETHYL KETONE at 1.15 A resolution Deposited 2015-04-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
106–384（279 aa）
Fragment：unp residues 106-384
|
Not recorded | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；Ammonium Sulfate , Tris HCL, calcium chloride
|
Resolution 1.15 Å R-free 0.135 |
| 5AMX Crystal Structure of Proteinase K processed with the CrystalDirect automated mounting and cryo-cooling technology Deposited 2015-09-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP RESIDUES 106-384
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
0.1 M HEPES, PH=7.5, 1.3 M AMMONIUM SULFATE, 0.1 M NACL
|
Resolution 1.01 Å R-free 0.166 |
| 5AVJ Crystal structure of proteinase K from Engyodontium album Deposited 2015-06-17 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | NO3 NITRATE ION × 4 GOL GLYCEROL × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；291 K；NaNO3, CaCl2, MES
|
Resolution 1.45 Å R-free 0.184 |
| 5AVK Crystal structure of proteinase K from Engyodontium album Deposited 2015-06-17 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | NO3 NITRATE ION × 4 GOL GLYCEROL × 1 PR PRASEODYMIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；291 K；NaNO3, PrCl3
|
Resolution 1.45 Å R-free 0.194 |
| 5B1D Crystal structure of proteinase K from Engyodontium album Deposited 2015-12-03 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；291 K；NaNO3, CaCl2, MES
|
Resolution 2.30 Å R-free 0.183 |
| 5B1E Crystal structure of proteinase K from Engyodontium album Deposited 2015-12-03 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；291 K；NaNO3, CaCl2, MES
|
Resolution 2.30 Å R-free 0.181 |
| 5CW1 Proteinase K complexed with 4-iodopyrazole Deposited 2015-07-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | SO4 SULFATE ION × 2 PYZ 4-IODOPYRAZOLE × 3 IOD IODIDE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；100mM Tris pH7.2 and 1.28 M ammonium sulfate
|
Resolution 1.45 Å R-free 0.153 |
| 5I9S MicroED structure of proteinase K at 1.75 A resolution Deposited 2016-02-20 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 2 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.75 Å R-free 0.266 |
| 5K7S MicroED structure of proteinase K at 1.6 A resolution Deposited 2016-05-26 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.60 Å R-free 0.255 |
| 5KXU Structure Proteinase K determined by SACLA Deposited 2016-07-20 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；293 K；0.1 M MES-NaOH (pH 6.5), 0.5 M NaNO3, 0.1 M CaCl2
|
Resolution 1.20 Å R-free 0.129 |
| 5KXV Structure Proteinase K at 0.98 Angstroms Deposited 2016-07-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | NO3 NITRATE ION × 1 GOL GLYCEROL × 2 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 6.5；293 K；250 mM NaNO3, 50 mM CaCl2, 50 mM MES-NaOH
|
Resolution 0.98 Å R-free 0.114 |
| 5MJL Single-shot pink beam serial crystallography: Proteinase K Deposited 2016-12-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 CL CHLORIDE ION × 1 NHE 2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；293 K；1.6M MgSO4, 10 mM CaCl2, 100 mM CHC buffer pH 6.5
|
Resolution 2.21 Å R-free 0.195 |
| 5ROC PanDDA analysis group deposition -- Proteinase K crystal structure Apo65 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.52 Å R-free 0.354 |
| 5ROD PanDDA analysis group deposition -- Proteinase K crystal structure Apo71 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.04 Å R-free 0.189 |
| 5ROE PanDDA analysis group deposition -- Proteinase K crystal structure Apo6 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.50 Å R-free 0.334 |
| 5ROF PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen C11a Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 4AQ 2-(1H-indol-3-yl)-N-[(1-methyl-1H-pyrrol-2-yl)methyl]ethanamine × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.08 Å R-free 0.190 |
| 5ROG PanDDA analysis group deposition -- Proteinase K crystal structure Apo51 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.08 Å R-free 0.173 |
| 5ROH PanDDA analysis group deposition -- Proteinase K crystal structure Apo61 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.04 Å R-free 0.176 |
| 5ROI PanDDA analysis group deposition -- Proteinase K crystal structure Apo30 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.05 Å R-free 0.168 |
| 5ROJ PanDDA analysis group deposition -- Proteinase K crystal structure Apo59 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.02 Å R-free 0.150 |
| 5ROK PanDDA analysis group deposition -- Proteinase K crystal structure Apo37 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.04 Å R-free 0.159 |
| 5ROL PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen B5a Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 47S 3-(dimethylamino)benzohydrazide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.22 Å R-free 0.176 |
| 5ROM PanDDA analysis group deposition -- Proteinase K crystal structure Apo45 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.07 Å R-free 0.176 |
| 5RON PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen E4a Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 483 2-(1H-imidazol-1-yl)-N-(trans-4-methylcyclohexyl)acetamide × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.22 Å R-free 0.166 |
| 5ROO PanDDA analysis group deposition -- Proteinase K crystal structure Apo73 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.41 Å R-free 0.222 |
| 5ROP PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen A12a at Room Temperature Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 3 47E (2R)-(3-chlorophenyl)(hydroxy)ethanoic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.64 Å R-free 0.234 |
| 5ROQ PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen A12a Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 47E (2R)-(3-chlorophenyl)(hydroxy)ethanoic acid × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.02 Å R-free 0.161 |
| 5ROR PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen F1a Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 NCA NICOTINAMIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.22 Å R-free 0.166 |
| 5ROS PanDDA analysis group deposition -- Proteinase K crystal structure Apo34 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.45 Å R-free 0.390 |
| 5ROT PanDDA analysis group deposition -- Proteinase K crystal structure Apo1 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.05 Å R-free 0.170 |
| 5ROU PanDDA analysis group deposition -- Proteinase K crystal structure Apo72 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.06 Å R-free 0.178 |
| 5ROV PanDDA analysis group deposition -- Proteinase K crystal structure Apo62 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.04 Å R-free 0.197 |
| 5ROW PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen F6a Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 CLW CHLORZOXAZONE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.21 Å R-free 0.283 |
| 5ROX PanDDA analysis group deposition -- Proteinase K crystal structure Apo24 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.12 Å R-free 0.179 |
| 5ROY PanDDA analysis group deposition -- Proteinase K crystal structure Apo22 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.05 Å R-free 0.169 |
| 5ROZ PanDDA analysis group deposition -- Proteinase K crystal structure Apo41 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.14 Å R-free 0.368 |
| 5RP0 PanDDA analysis group deposition -- Proteinase K crystal structure Apo57 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.03 Å R-free 0.163 |
| 5RP1 PanDDA analysis group deposition -- Proteinase K crystal structure Apo36 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.11 Å R-free 0.287 |
| 5RP2 PanDDA analysis group deposition -- Proteinase K crystal structure Apo20 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.10 Å R-free 0.177 |
| 5RP3 PanDDA analysis group deposition -- Proteinase K crystal structure Apo14 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.09 Å R-free 0.173 |
| 5RP4 PanDDA analysis group deposition -- Proteinase K crystal structure Apo70 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.05 Å R-free 0.185 |
| 5RP5 PanDDA analysis group deposition -- Proteinase K crystal structure Apo48 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.09 Å R-free 0.184 |
| 5RP6 PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen C2a Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 46P 4-methyl-5-(1-methyl-1H-imidazol-2-yl)-1,3-thiazol-2-amine × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.17 Å R-free 0.165 |
| 5RP7 PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen D12a Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 F91 N-(pyridin-4-ylmethyl)-2,3-dihydro-1,4-benzodioxin-6-amine × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.15 Å R-free 0.197 |
| 5RP8 PanDDA analysis group deposition -- Proteinase K crystal structure Apo46 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.03 Å R-free 0.188 |
| 5RP9 PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen B7a Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 YEY 2-(2,3-dihydro-1,4-benzodioxin-6-yl)-1H-pyrrole × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.48 Å R-free 0.182 |
| 5RPA PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen E3a Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 47Y 3-[(4E)-4-imino-5,6-dimethylfuro[2,3-d]pyrimidin-3(4H)-yl]-N,N-dimethylpropan-1-amine × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.25 Å R-free 0.161 |
| 5RPB PanDDA analysis group deposition -- Proteinase K crystal structure Apo68 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.64 Å R-free 0.231 |
| 5RPC PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen H3a Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | W9J N-carbamoyl-L-methionine × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.23 Å R-free 0.188 |
| 5RPD PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen F12a Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 GGB L-CANAVANINE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.02 Å R-free 0.164 |
| 5RPE PanDDA analysis group deposition -- Proteinase K crystal structure Apo40 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.02 Å R-free 0.166 |
| 5RPF PanDDA analysis group deposition -- Proteinase K crystal structure Apo27 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.39 Å R-free 0.310 |
| 5RPG PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen H2a Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 AMH TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.50 Å R-free 0.158 |
| 5RPH PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen A11a Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 L2K 3-[3,4-bis(fluoranyl)phenyl]-1,4,6,7-tetrahydroimidazo[2,1-c][1,2,4]triazine × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.38 Å R-free 0.157 |
| 5RPI PanDDA analysis group deposition -- Proteinase K crystal structure Apo26 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.03 Å R-free 0.179 |
| 5RPJ PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen B12a Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 479 4-oxo-N-[(1S)-1-(pyridin-3-yl)ethyl]-4-(thiophen-2-yl)butanamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.27 Å R-free 0.176 |
| 5RPK PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen B9a Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 MRZ piperidine-1-carboximidamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.78 Å R-free 0.251 |
| 5RPL PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen C8a Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 8G2 (4-methoxycarbonylphenyl)methylazanium × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.09 Å R-free 0.192 |
| 5RPM PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen H5a Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 HBD 4-HYDROXYBENZAMIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.22 Å R-free 0.173 |
| 5RPN PanDDA analysis group deposition -- Proteinase K crystal structure Apo64 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.02 Å R-free 0.166 |
| 5RPO PanDDA analysis group deposition -- Proteinase K crystal structure Apo7 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.50 Å R-free 0.338 |
| 5RPP PanDDA analysis group deposition -- Proteinase K crystal structure Apo44 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.08 Å R-free 0.175 |
| 5RPQ PanDDA analysis group deposition -- Proteinase K crystal structure Apo32 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.07 Å R-free 0.173 |
| 5RPR PanDDA analysis group deposition -- Proteinase K crystal structure Apo15 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.16 Å R-free 0.214 |
| 5RPS PanDDA analysis group deposition -- Proteinase K crystal structure Apo60 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.04 Å R-free 0.172 |
| 5RPT PanDDA analysis group deposition -- Proteinase K crystal structure Apo9 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.98 Å R-free 0.283 |
| 5RPU PanDDA analysis group deposition -- Proteinase K crystal structure Apo67 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.55 Å R-free 0.228 |
| 5RPV PanDDA analysis group deposition -- Proteinase K crystal structure Apo35 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.10 Å R-free 0.177 |
| 5RPW PanDDA analysis group deposition -- Proteinase K crystal structure Apo63 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.02 Å R-free 0.171 |
| 5RPX PanDDA analysis group deposition -- Proteinase K crystal structure Apo43 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.05 Å R-free 0.170 |
| 5RPY PanDDA analysis group deposition -- Proteinase K crystal structure Apo10 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.09 Å R-free 0.186 |
| 5RPZ PanDDA analysis group deposition -- Proteinase K crystal structure Apo58 Deposited 2020-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；290 K；1.2M ammonium sulfate, 0.1M Tris-HCl, pH 8.0
|
Resolution 1.22 Å R-free 0.180 |
| 5UVL Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-crystals using Synchrotron Radiation Deposited 2017-02-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | NO3 NITRATE ION × 2 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；293 K；0.1 M MES pH 6.5, 0.5 M sodium nitrate, 0.1 M calcium chloride
|
Resolution 2.65 Å R-free 0.243 |
| 5WHW Using sound pulses to solve the crystal harvesting bottleneck Deposited 2017-07-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：residues 106-384
|
Not recorded | CA CALCIUM ION × 2 NO3 NITRATE ION × 1 EDO 1,2-ETHANEDIOL × 3 BCN BICINE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；1.6 M sodium nitrate, 0.16 M calcium chloride, 0.2 M bis-tris pH 6.5
|
Resolution 1.71 Å R-free 0.173 |
| 5WJG Using sound pulses to solve the crystal harvesting bottleneck Deposited 2017-07-22 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 TLA L(+)-TARTARIC ACID × 1 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；295 K；0.1 M BisTris pH 6.5, 0.08M CaCl2
|
Resolution 1.50 Å R-free 0.157 |
| 5WJH Using sound pulses to solve the crystal harvesting bottleneck Deposited 2017-07-22 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 MHA (CARBAMOYLMETHYL-CARBOXYMETHYL-AMINO)-ACETIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；295 K；0.1 M BisTris pH 6.5, 0.08M CaCl2
|
Resolution 1.63 Å R-free 0.249 |
| 5WRC Crystal structure of proteinase K from Engyodontium album Deposited 2016-12-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | NO3 NITRATE ION × 1 PR PRASEODYMIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；291 K；NaNO3
|
Resolution 1.50 Å R-free 0.193 |
| 6CL7 1.71 A MicroED structure of proteinase K at 0.86 e- / A^2 Deposited 2018-03-02 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.71 Å R-free 0.253 |
| 6CL8 2.00 A MicroED structure of proteinase K at 2.6 e- / A^2 Deposited 2018-03-02 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.00 Å R-free 0.259 |
| 6CL9 2.20 A MicroED structure of proteinase K at 4.3 e- / A^2 Deposited 2018-03-02 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.20 Å R-free 0.302 |
| 6CLA 2.80 A MicroED structure of proteinase K at 6.0 e- / A^2 Deposited 2018-03-02 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å R-free 0.326 |
| 6CLB 3.20 A MicroED structure of proteinase K at 7.8 e- / A^2 Deposited 2018-03-02 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å R-free 0.326 |
| 6FJS Proteinase~K SIRAS phased structure of room-temperature, serially collected synchrotron data Deposited 2018-01-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；298 K；50 mM Tris-HCl pH 7.0 with 10 mM CaCl2, 0.6 M NaNO3
|
Resolution 1.90 Å R-free 0.171 |
| 6J43 Proteinase K determined by PAL-XFEL Deposited 2019-01-07 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Mutation：S312D | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；291 K；0.25 M sodium nitrate, 0.05 M calcium chloride, 0.1 M MES (pH 6.5)
|
Resolution 1.85 Å R-free 0.239 |
| 6K2P Crystal structure of proteinase K from Engyodontium album Deposited 2019-05-15 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Mutation：S312D | CA CALCIUM ION × 2 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；293 K；0.5 M NaNO3, 0.1 M CaCl2, 0.1 M MES
|
Resolution 1.60 Å R-free 0.181 |
| 6K2R Crystal structure of proteinase K from Engyodontium album Deposited 2019-05-15 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Mutation：S312D | CA CALCIUM ION × 2 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；293 K；0.5 M NaNO3, 0.1 M CaCl2, 0.1 M MES
|
Resolution 1.60 Å R-free 0.190 |
| 6K2T Crystal structure of proteinase K from Engyodontium album Deposited 2019-05-15 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Mutation：S312D | CA CALCIUM ION × 2 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；293 K；0.5 M NaNO3, 0.1 M CaCl2, 0.1 M MES
|
Resolution 1.60 Å R-free 0.186 |
| 6K2V Crystal structure of proteinase K from Engyodontium album Deposited 2019-05-15 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Mutation：S312D | CA CALCIUM ION × 2 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；293 K；0.5 M NaNO3, 0.1 M CaCl2, 0.1 M MES
|
Resolution 1.60 Å R-free 0.184 |
| 6K2W Crystal structure of proteinase K from Engyodontium album Deposited 2019-05-15 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Mutation：S312D | CA CALCIUM ION × 2 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；293 K；0.5 M NaNO3, 0.1 M CaCl2, 0.1 M MES
|
Resolution 1.70 Å R-free 0.183 |
| 6K2X Crystal structure of proteinase K from Engyodontium album Deposited 2019-05-15 | Different ligand/ion Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Mutation：S312D | PR PRASEODYMIUM ION × 2 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；293 K；0.5 M NaNO3, 0.1 M CaCl2, 0.1 M MES
|
Resolution 1.65 Å R-free 0.181 |
| 6K8M High resolution crystal structure of proteinase K with thiourea Deposited 2019-06-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
106–384（279 aa）
|
Mutation：S207D | TOU THIOUREA × 3 SO4 SULFATE ION × 1 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；273 K；1.28M ammonium sulfate, 0.1M Tris-HCl, pH 7.2
|
Resolution 1.45 Å R-free 0.179 |
| 6KKF Crystal structure of proteinase K complexed with a triglycine Deposited 2019-07-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | CA CALCIUM ION × 2 GGG glycylglycylglycine × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；291.5 K；Tris-HCl, ammonium sulfate
|
Resolution 1.40 Å R-free 0.218 |
| 6LAW MicroED structure of proteinase K at 1.50A determained using crystal lamellas prepared by focused ion beam milling Deposited 2019-11-13 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.50 Å R-free 0.225 |
| 6MH6 High-viscosity injector-based Pink Beam Serial Crystallography of Micro-crystals at a Synchrotron Radiation Source. Deposited 2018-09-17 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；293 K；0.1 M MES pH 6.5, 0.5 M sodium nitrate, 0.1 M calcium chloride
|
Resolution 1.80 Å R-free 0.175 |
| 6N4U MicroED structure of Proteinase K at 2.75A resolution from a single milled crystal. Deposited 2018-11-20 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 SO4 SULFATE ION × 1 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.75 Å R-free 0.263 |
| 6PKJ MicroED structure of proteinase K from an uncoated, single lamella at 2.17A resolution (#2) Deposited 2019-06-29 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.17 Å R-free 0.265 |
| 6PKK MicroED structure of proteinase K from an uncoated, single lamella at 2.18A resolution (#5) Deposited 2019-06-29 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.18 Å R-free 0.265 |
| 6PKL MicroED structure of proteinase K from an uncoated, single lamella at 2.59A resolution (#7) Deposited 2019-06-29 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.59 Å R-free 0.310 |
| 6PKM MicroED structure of proteinase K from an uncoated, single lamella at 2.17A resolution (#8) Deposited 2019-06-29 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.17 Å R-free 0.284 |
| 6PKN MicroED structure of proteinase K from an unpolished, platinum-coated, single lamella at 2.08A resolution (#9) Deposited 2019-06-29 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.08 Å R-free 0.258 |
| 6PKO MicroED structure of proteinase K from a platinum coated, unpolished, single lamella at 2.07A resolution (#12) Deposited 2019-06-29 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.07 Å R-free 0.269 |
| 6PKP MicroED structure of proteinase K from a platinum-coated, polished, single lamella at 1.91A resolution (#10) Deposited 2019-06-29 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.91 Å R-free 0.234 |
| 6PKQ MicroED structure of proteinase K from a platinum-coated, polished, single lamella at 1.85A resolution (#11) Deposited 2019-06-29 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.85 Å R-free 0.234 |
| 6PKR MicroED structure of proteinase K from a platinum-coated, polished, single lamella at 1.79A resolution (#13) Deposited 2019-06-29 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.79 Å R-free 0.236 |
| 6PKS MicroED structure of proteinase K from low-dose merged lamellae that were not pre-coated with platinum 2.16A resolution (LD) Deposited 2019-06-29 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.16 Å R-free 0.252 |
| 6PKT MicroED structure of proteinase K from merging low-dose, platinum pre-coated lamellae at 1.85A resolution (LDPT) Deposited 2019-06-29 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.85 Å R-free 0.225 |
| 6PQ0 LCP-embedded Proteinase K treated with MPD Deposited 2019-07-08 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | CA CALCIUM ION × 2 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.00 Å R-free 0.267 |
| 6PQ4 LCP-embedded Proteinase K treated with lipase Deposited 2019-07-08 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | CA CALCIUM ION × 2 NO3 NITRATE ION × 1 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.00 Å R-free 0.282 |
| 6PU4 MicroED structure of proteinase K recorded on Falcon III Deposited 2019-07-17 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | CA CALCIUM ION × 4 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.10 Å R-free 0.267 |
| 6PU5 MicroED structure of proteinase K recorded on CetaD Deposited 2019-07-17 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
Fragment：UNP residues 106-384
|
Not recorded | CA CALCIUM ION × 2 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å R-free 0.266 |
| 6QF1 X-Ray structure of Proteinase K crystallized on a silicon chip Deposited 2019-01-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 NHE 2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；295 K；0.1M CHC buffer pH 6.5, 0.6M ammonium sulphate, 10mM calcium chloride
|
Resolution 1.74 Å R-free 0.156 |
| 6QXV Pink beam serial crystallography: Proteinase K, 1 us exposure, 1585 patterns merged (2 chips) Deposited 2019-03-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 SO4 SULFATE ION × 11 CL CHLORIDE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.6；298 K；0.1M CHC buffer pH 6.6, 0.7M ammonium sulphate and 10mM calcium chloride
|
Resolution 1.94 Å R-free 0.227 |
| 6RUG Co-substituted alpha-Keggin bound to Proteinase K solved by MR Deposited 2019-05-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | WCO Co-substituted alpha-Keggin × 2 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100 mM sodium acetate (pH 5.5), 0.7-1.2 M ammonium sulphate, 2.5 mM Co-substituted alpha-Keggin
|
Resolution 1.10 Å R-free 0.154 |
| 6RUH Ni-substituted alpha-Keggin bound to Proteinase K solved by MR Deposited 2019-05-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | WNI Ni-substituted alpha-Keggin × 2 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100 mM sodium acetate (pH 5.5), 0.7-1.2 M ammonium sulphate, 5 mM Ni-subsituted alpha-Keggin
|
Resolution 1.10 Å R-free 0.158 |
| 6RUK Cu-substituted alpha-Keggin bound to Proteinase K solved by MR Deposited 2019-05-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | XCU Cu-substituted alpha-Keggin × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100 mM sodium acetate (pH 5.5), 0.7-1.2 M ammonium sulphate, 5 mM Cu-substituted alpha-Keggin
|
Resolution 1.20 Å R-free 0.167 |
| 6RUN Co-substituted alpha-Keggin bound to Proteinase K solved by EP Deposited 2019-05-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | WCO Co-substituted alpha-Keggin × 2 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100 mM sodium acetate (pH 5.5), 0.7-1.2 M ammonium sulphate, 2.5 mM Co-subsituted alpha-Keggin
|
Resolution 1.10 Å R-free 0.140 |
| 6RUW Zn-substituted alpha-Keggin bound to Proteinase K solved by MR Deposited 2019-05-29 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | KK5 Zn-substituted alpha-Keggin × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100 mM sodium acetate (pH 5.5), 0.7-1.2 M ammonium sulphate, 2.5 mM Zn-substituted alpha-Keggin
|
Resolution 1.35 Å R-free 0.181 |
| 6RVE Co-substituted beta-Keggin bound to Proteinase K solved by MR Deposited 2019-05-31 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | XCO Co-substituted beta-Keggin × 1 KCO Co-substituted beta-Keggin × 1 SO4 SULFATE ION × 1 BET TRIMETHYL GLYCINE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；100 mM sodium acetate (pH 4.5), 0.3-0.7 M ammonium acetate, 0.5 M betaine, 10 mM Co-substituted beta-Keggin
|
Resolution 1.15 Å R-free 0.218 |
| 6RVG Co-substituted beta-Keggin bound to Proteinase K solved by MR Deposited 2019-05-31 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | XCO Co-substituted beta-Keggin × 3 KCO Co-substituted beta-Keggin × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；100 mM sodium acetate (pH 4.5), 0.3-0.7 M ammonium sulphate, 0.5 M betaine, 10 mM Co-substituted beta-Keggin
|
Resolution 1.10 Å R-free 0.200 |
| 6RZP Multicrystal structure of Proteinase K at room temperature using a multilayer monochromator. Deposited 2019-06-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | GOL GLYCEROL × 1 SO4 SULFATE ION × 2 CA CALCIUM ION × 1 CL CHLORIDE ION × 1 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；1.2M Ammonium Sulfate, 0.1M Na Cacodylate pH 6.2, 25 % Glycerol
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；1.2M Ammonium Sulfate, 0.1M Na Cacodylate pH 6.2, 25 % Glycerol
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；1.2M Ammonium Sulfate, 0.1M Na Cacodylate pH 6.2, 25 % Glycerol
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；1.2M Ammonium Sulfate, 0.1M Na Cacodylate pH 6.2, 25 % Glycerol
|
Resolution 2.20 Å R-free 0.161 |
| 6TXG Proteinase K in complex with a "half sandwich"-type Ru(II) coordination compound Deposited 2020-01-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | NO3 NITRATE ION × 3 EDO 1,2-ETHANEDIOL × 2 CA CALCIUM ION × 1 NYN chlorido(1,2-diaminoethane-k2N,N')(1,4,7-trithiacyclononane-k3S,S',S'')ruthenium(II) trifluoromethanesulfonate × 2 NA SODIUM ION × 1 TFS trifluoromethanesulfonic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；289 K；1M SODIUM NITRATE, 0.1M CITRATE BUFFER, pH=6.5
|
Resolution 1.37 Å R-free 0.168 |
| 6V8R Proteinase K Determined by MicroED Phased by ARCIMBOLDO_SHREDDER Deposited 2019-12-11 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.60 Å R-free 0.232 |
| 6ZET Crystal structure of proteinase K nanocrystals by electron diffraction with a 20 micrometre C2 condenser aperture Deposited 2020-06-16 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain AAA
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Excess liquid was removed by blotting for 4-6s with a Vitrobot
|
Resolution 2.70 Å R-free 0.268 |
| 6ZEU Crystal structure of proteinase K lamella by electron diffraction with a 50 micrometre C2 condenser aperture Deposited 2020-06-16 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain AAA
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Excess liquid was removed by blotting for 4-6s with a Vitrobot
|
Resolution 2.00 Å R-free 0.234 |
| 6ZEV Crystal structure of proteinase K lamellae by electron diffraction with a 20 micrometre C2 condenser aperture Deposited 2020-06-16 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain AAA
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Excess liquid was removed by blotting for 4-6s with a Vitrobot
|
Resolution 2.40 Å R-free 0.243 |
| 7A68 proteinase K crystallized from 0.5 M NaNO3 Deposited 2020-08-25 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | NA SODIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；Protein concentration: 10mg/ml. 0,2 M Tris pH 8, 0.5 M NaNO3
|
Resolution 2.55 Å R-free 0.200 |
| 7A9F Co-substituted Keggin silicotungstate with covalent bond to proteinase K Deposited 2020-09-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | R5Q Co-substituted Keggin silicotungstate × 2 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100 mM NaOAc/AcOH
0.7 - 1.1 M (NH4)2SO4
5 mM polyoxometalate
|
Resolution 1.62 Å R-free 0.174 |
| 7A9K Co-substituted Keggin silicotungstate with covalent bond to proteinase K Deposited 2020-09-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 R5Q Co-substituted Keggin silicotungstate × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100 mM NaOAc/AcOH (pH 5.5)
0.7 - 1.1 M (NH4)2SO4
5 mM polyoxometalate
|
Resolution 1.62 Å R-free 0.166 |
| 7A9M Ni-substituted Keggin silicotungstate with covalent bond to proteinase K Deposited 2020-09-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 R5N Ni-substituted Keggin silicotungstate × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100 mM NaOAc/AcOH (pH 5.5)
0.2 - 0.5 M (NH4)2SO4
0.5 M betaine
5 mM polyoxometalate
|
Resolution 1.62 Å R-free 0.190 |
| 7C0P Structure of proteinase K obtained in SSRF using serial crystallography Deposited 2020-05-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；293 K；PEG 20000, Tris-HCl pH 8.5, Ammonium sulfate
|
Resolution 2.15 Å R-free 0.207 |
| 7JSY Proteinase K soaked with I3C determined by MicroED from a single milled microcrystal Deposited 2020-08-16 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | I3C 5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid × 4 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.78 Å R-free 0.209 |
| 7LN7 X-ray radiation damage series on Proteinase K at 277K, crystal structure, dataset 1 Deposited 2021-02-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 3 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Proteinase K was dissolved at a final concentration of 30 mg/ml in 10mM Calcium Chloride, 50 mM Tris pH 7.5. 1-2 microliters of this protein solution was mixed with an equivalent volume of precipitant solution from the well (1.0 M Ammonium Sulfate).
|
Resolution 1.02 Å R-free 0.138 |
| 7LPT X-ray radiation damage series on Proteinase K at 277K, crystal structure, dataset 4 Deposited 2021-02-12 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 3 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Proteinase K was dissolved in 10 mM Calcium Chloride, 50 mM Tris pH 7.5 at a final concentration of 30 mg/ml. 1-2 microliters of this protein solution was mixed with an equivalent volume of precipitant solution (1.0 M Ammonium Sulfate).
|
Resolution 1.43 Å R-free 0.162 |
| 7LPU X-ray radiation damage series on Proteinase K at 277K, multi-conformer model, dataset 1 Deposited 2021-02-12 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 3 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Proteinase K was dissolved in 10 mM Calcium Chloride, 50 mM Tris pH 7.5 at a final concentration of 30 mg/ml. 1-2 microliters of this protein solution was mixed with an equivalent volume of precipitant solution (1.0 M Ammonium Sulfate).
|
Resolution 1.02 Å R-free 0.138 |
| 7LPV X-ray radiation damage series on Proteinase K at 277K, multi-conformer model, dataset 2 Deposited 2021-02-12 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 3 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Proteinase K was dissolved in 10 mM Calcium Chloride, 50 mM Tris pH 7.5 at a final concentration of 30 mg/ml. 1-2 microliters of this protein solution was mixed with an equivalent volume of precipitant solution (1.0 M Ammonium Sulfate).
|
Resolution 1.10 Å R-free 0.146 |
| 7LQ8 X-ray radiation damage series on Proteinase K at 277K, multi-conformer model, dataset 3 Deposited 2021-02-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 3 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Proteinase K was dissolved in 10 mM Calcium Chloride, 50 mM Tris pH 7.5 at a final concentration of 30 mg/ml. 1-2 microliters of this protein solution was mixed with an equivalent volume of precipitant solution (1.0 M Ammonium Sulfate).
|
Resolution 1.30 Å R-free 0.151 |
| 7LQ9 X-ray radiation damage series on Proteinase K at 277K, multi-conformer model, dataset 4 Deposited 2021-02-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 3 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Proteinase K was dissolved in 10 mM Calcium Chloride, 50 mM Tris pH 7.5 at a final concentration of 30 mg/ml. 1-2 microliters of this protein solution was mixed with an equivalent volume of precipitant solution (1.0 M Ammonium Sulfate).
|
Resolution 1.43 Å R-free 0.162 |
| 7LQA X-ray radiation damage series on Proteinase K at 277K, multi-conformer model, dataset 2 (merged) Deposited 2021-02-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 3 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Proteinase K was dissolved in 10 mM Calcium Chloride, 50 mM Tris pH 7.5 at a final concentration of 30 mg/ml. 1-2 microliters of this protein solution was mixed with an equivalent volume of precipitant solution (1.0 M Ammonium Sulfate).
|
Resolution 1.02 Å R-free 0.127 |
| 7LQB X-ray radiation damage series on Proteinase K at 277K, multi-conformer model, dataset 3 (merged) Deposited 2021-02-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 3 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Proteinase K was dissolved in 10 mM Calcium Chloride, 50 mM Tris pH 7.5 at a final concentration of 30 mg/ml. 1-2 microliters of this protein solution was mixed with an equivalent volume of precipitant solution (1.0 M Ammonium Sulfate).
|
Resolution 1.02 Å R-free 0.128 |
| 7LQC X-ray radiation damage series on Proteinase K at 277K, multi-conformer model, dataset 4 (merged) Deposited 2021-02-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 3 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Proteinase K was dissolved in 10 mM Calcium Chloride, 50 mM Tris pH 7.5 at a final concentration of 30 mg/ml. 1-2 microliters of this protein solution was mixed with an equivalent volume of precipitant solution (1.0 M Ammonium Sulfate).
|
Resolution 1.02 Å R-free 0.128 |
| 7LTD X-ray radiation damage series on Proteinase K at 100K, crystal structure, dataset 1 Deposited 2021-02-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 NO3 NITRATE ION × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Proteinase K was dissolved in 10 mM Calcium Chloride, 50 mM Tris pH 7.5 at a final concentration of 30 mg/ml. 1-2 microliters of this protein solution was mixed with an equivalent volume of precipitant solution (0.5 M Sodium Nitrate).
|
Resolution 0.90 Å R-free 0.172 |
| 7LTI X-ray radiation damage series on Proteinase K at 100K, crystal structure, dataset 2 Deposited 2021-02-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 NO3 NITRATE ION × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Proteinase K was dissolved in 10 mM Calcium Chloride, 50 mM Tris pH 7.5 at a final concentration of 30 mg/ml. 1-2 microliters of this protein solution was mixed with an equivalent volume of precipitant solution (0.5 M Sodium Nitrate).
|
Resolution 0.91 Å R-free 0.170 |
| 7LTV X-ray radiation damage series on Proteinase K at 100K, crystal structure, dataset 3 Deposited 2021-02-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 NO3 NITRATE ION × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Proteinase K was dissolved in 10 mM Calcium Chloride, 50 mM Tris pH 7.5 at a final concentration of 30 mg/ml. 1-2 microliters of this protein solution was mixed with an equivalent volume of precipitant solution (0.5 M Sodium Nitrate).
|
Resolution 0.95 Å R-free 0.170 |
| 7LU0 X-ray radiation damage series on Proteinase K at 100K, crystal structure, dataset 4 Deposited 2021-02-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 NO3 NITRATE ION × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Proteinase K was dissolved in 10 mM Calcium Chloride, 50 mM Tris pH 7.5 at a final concentration of 30 mg/ml. 1-2 microliters of this protein solution was mixed with an equivalent volume of precipitant solution (0.5 M Sodium Nitrate).
|
Resolution 1.01 Å R-free 0.168 |
| 7LU1 X-ray radiation damage series on Proteinase K at 100K, crystal structure, dataset 5 Deposited 2021-02-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 NO3 NITRATE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Proteinase K was dissolved in 10 mM Calcium Chloride, 50 mM Tris pH 7.5 at a final concentration of 30 mg/ml. 1-2 microliters of this protein solution was mixed with an equivalent volume of precipitant solution (0.5 M Sodium Nitrate).
|
Resolution 1.06 Å R-free 0.169 |
| 7LU2 X-ray radiation damage series on Proteinase K at 100K, crystal structure, dataset 6 Deposited 2021-02-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 NO3 NITRATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Proteinase K was dissolved in 10 mM Calcium Chloride, 50 mM Tris pH 7.5 at a final concentration of 30 mg/ml. 1-2 microliters of this protein solution was mixed with an equivalent volume of precipitant solution (0.5 M Sodium Nitrate).
|
Resolution 1.11 Å R-free 0.173 |
| 7LU3 X-ray radiation damage series on Proteinase K at 100K, crystal structure, dataset 7 Deposited 2021-02-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 NO3 NITRATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Proteinase K was dissolved in 10 mM Calcium Chloride, 50 mM Tris pH 7.5 at a final concentration of 30 mg/ml. 1-2 microliters of this protein solution was mixed with an equivalent volume of precipitant solution (0.5 M Sodium Nitrate).
|
Resolution 1.16 Å R-free 0.172 |
| 7NJJ Proteinase K grown inside HARE serial crystallography chip Deposited 2021-02-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；1 M NaNO3, 0.1 M sodium citrate pH 6.5
|
Resolution 1.65 Å R-free 0.203 |
| 7NUY New polymorhp of proteinase K obtained by free interface diffusion technique Deposited 2021-03-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIQUID DIFFUSION；pH 8；293.5 K；Free interface diffusion: 1.8 M (NH4)2SO4, 0.1 M Tris pH 8.0
|
Resolution 1.65 Å R-free 0.221 |
| 7NUZ Proteinase K structure at atomic resolution from crystals grown in agarose gel Deposited 2021-03-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | NO3 NITRATE ION × 1 NA SODIUM ION × 2 GOL GLYCEROL × 2 SO4 SULFATE ION × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
COUNTER-DIFFUSION；pH 6.5；293.5 K；0.5 M NaNO3, 0.025 M Na citrate (pH 6.5)
|
Resolution 1.09 Å R-free 0.191 |
| 7S4Z Serial Macromolecular Crystallography at ALBA Synchrotron Light Source - Proteinase K Deposited 2021-09-09 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | NO3 NITRATE ION × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；298 K；0.1 M MES pH 6.5, 0.5 sodium citrate, 0.1 M calcium chloride
|
Resolution 1.90 Å R-free 0.196 |
| 7SKX Ab initio structure of proteinase K from electron-counted MicroED data Deposited 2021-10-21 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
106–384（279 aa）
|
Not recorded | I3C 5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid × 2 CA CALCIUM ION × 5 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.50 Å R-free 0.205 |
| 7SVY MicroED structure of proteinase K from a 130 nm thick lamella measured at 120 kV Deposited 2021-11-19 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.30 Å R-free 0.261 |
| 7SVZ MicroED structure of proteinase K from a 200 nm thick lamella measured at 120 kV Deposited 2021-11-19 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.00 Å R-free 0.243 |
| 7SW0 MicroED structure of proteinase K from a 325 nm thick lamella measured at 120 kV Deposited 2021-11-19 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å R-free 0.285 |
| 7SW1 MicroED structure of proteinase K from a 115 nm thick lamella measured at 200 kV Deposited 2021-11-19 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.85 Å R-free 0.217 |
| 7SW2 MicroED structure of proteinase K from a 130 nm thick lamella measured at 200 kV Deposited 2021-11-19 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.95 Å R-free 0.236 |
| 7SW3 MicroED structure of proteinase K from a 95 nm thick lamella measured at 200 kV Deposited 2021-11-19 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.35 Å R-free 0.240 |
| 7SW4 MicroED structure of proteinase K from a 540 nm thick lamella measured at 200 kV Deposited 2021-11-19 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å R-free 0.222 |
| 7SW5 MicroED structure of proteinase K from a 460 nm thick lamella measured at 200 kV Deposited 2021-11-19 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.95 Å R-free 0.245 |
| 7SW6 MicroED structure of proteinase K from a 260 nm thick lamella measured at 200 kV Deposited 2021-11-19 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.95 Å R-free 0.238 |
| 7SW7 MicroED structure of proteinase K from a 530 nm thick lamella measured at 200 kV Deposited 2021-11-19 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.30 Å R-free 0.242 |
| 7SW8 MicroED structure of proteinase K from a 150 nm thick lamella measured at 300 kV Deposited 2021-11-19 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.90 Å R-free 0.226 |
| 7SW9 MicroED structure of proteinase K from a 170 nm thick lamella measured at 300 kV Deposited 2021-11-19 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.10 Å R-free 0.332 |
| 7SWA MicroED structure of proteinase K from a 320 nm thick lamella measured at 300 kV Deposited 2021-11-19 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.10 Å R-free 0.245 |
| 7SWB MicroED structure of proteinase K from a 360 nm thick lamella measured at 300 kV Deposited 2021-11-19 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.05 Å R-free 0.236 |
| 7SWC MicroED structure of proteinase K from a 550 nm thick lamella measured at 300 kV Deposited 2021-11-19 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å R-free 0.275 |
| 8E52 MicroED structure of proteinase K recorded on K2 Deposited 2022-08-19 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain AAA
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 4 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å R-free 0.292 |
| 8E53 MicroED structure of proteinase K recorded on K3 Deposited 2022-08-19 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain AAA
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.70 Å R-free 0.254 |
| 8F05 Proteinase K Anomalous Dataset at 293 K and 7.1 keV Deposited 2022-11-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 7 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 uL 1 M ammonium sulfate + 2 uL 10 mg/mL proteinase K in 50 mM Tris, pH 7.5
|
Resolution 1.80 Å R-free 0.140 |
| 8F06 Proteinase K Anomalous Dataset at 310 K and 7.1 keV Deposited 2022-11-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 uL 1 M ammonium sulfate + 2 uL 10 mg/mL proteinase K in 50 mM Tris, pH 7.5
|
Resolution 1.80 Å R-free 0.147 |
| 8F07 Proteinase K Anomalous Dataset at 273 K and 12 keV Deposited 2022-11-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 9 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 uL 1 M ammonium sulfate + 2 uL 10 mg/mL proteinase K in 50 mM Tris, pH 7.5
|
Resolution 1.05 Å R-free 0.105 |
| 8FYO MicroED structure of Proteinase K from lamellae milled from multiple plasma sources Deposited 2023-01-26 | Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | NO3 NITRATE ION × 1 CA CALCIUM ION × 2 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.39 Å R-free 0.163 |
| 8FYP MicroED structure of Proteinase K from xenon milled lamellae Deposited 2023-01-26 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | NO3 NITRATE ION × 2 CA CALCIUM ION × 2 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.45 Å R-free 0.177 |
| 8FYQ MicroED structure of Proteinase K from argon milled lamellae Deposited 2023-01-26 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | NO3 NITRATE ION × 2 CA CALCIUM ION × 2 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.40 Å R-free 0.173 |
| 8FYR MicroED structure of Proteinase K from oxygen milled lamellae Deposited 2023-01-26 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | NO3 NITRATE ION × 2 CA CALCIUM ION × 2 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.50 Å R-free 0.214 |
| 8FYS MicroED structure of Proteinase K from nitrogen milled lamellae Deposited 2023-01-26 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.80 Å R-free 0.212 |
| 8RSE Proteinase K measured via serial crystallography from a silicon HARE-chip Deposited 2024-01-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–384（384 aa）
|
Not recorded | NO3 NITRATE ION × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；293 K；Microcrystals were obtained by mixing proteinase K solution (60mg/ml) with precipitant solution 1M NaNO3, 0.1M sodium
citrate pH 6.5, in a 5:1 ratio and left overnight at room temperature.
|
Resolution 1.66 Å R-free 0.178 |
| 8RSF Proteinase K measured via serial crystallography from a kapton HARE-chip (50 micron) Deposited 2024-01-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–384（384 aa）
|
Not recorded | NO3 NITRATE ION × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；293 K；Microcrystals were obtained by mixing proteinase K solution (60mg/ml) with precipitant solution 1M NaNO3, 0.1M sodium
citrate pH 6.5, in a 5:1 ratio and left overnight at room temperature.
|
Resolution 1.66 Å R-free 0.158 |
| 8RSG Proteinase K measured via serial crystallography from a kapton HARE-chip (125 micron) Deposited 2024-01-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–384（384 aa）
|
Not recorded | NO3 NITRATE ION × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；293 K；Microcrystals were obtained by mixing proteinase K solution (60mg/ml) with precipitant solution 1M NaNO3, 0.1M sodium
citrate pH 6.5, in a 5:1 ratio and left overnight at room temperature.
|
Resolution 1.66 Å R-free 0.179 |
| 8SDK The MicroED structure of proteinase K crystallized by suspended drop crystallization Deposited 2023-04-06 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 1 CA CALCIUM ION × 1 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.10 Å R-free 0.294 |
| 8SOG Proteinase K Multiconformer Model at 313K Deposited 2023-04-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 3 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Proteinase K was dissolved at pH 7.5 to 30 mg/mL in a 50 mM TRIS buffer. The protein was crystallized using a hanging drop setup on a 24 well VDX plate with sealant and 22 mm thick siliconized circle cover slides by mixing equal amounts of protein solution with 1.2 M ammonium sulfate.
|
Resolution 1.13 Å R-free 0.151 |
| 8SOU Proteinase K Multiconformer Model at 363K Deposited 2023-04-30 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 4 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Proteinase K was dissolved at pH 7.5 to 30 mg/mL in a 50 mM TRIS buffer . The protein was crystallized using a hanging drop setup on a 24 well VDX plate with sealant and 22 mm thick siliconized circle cover slides by mixing equal amounts of protein solution with ammonium sulfate.
|
Resolution 1.54 Å R-free 0.223 |
| 8SOV Proteinase K Multiconformer Model at 353K Deposited 2023-04-30 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 3 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Proteinase K was dissolved at pH 7.5 to 30 mg/mL in a 50 mM TRIS buffer. The protein was crystallized using a hanging drop setup on a 24 well VDX plate with sealant and 22 mm thick siliconized circle cover slides by mixing equal amounts of protein solution with 1.2 M ammonium sulfate.
|
Resolution 1.29 Å R-free 0.165 |
| 8SPL Proteinase K Multiconformer Model at 343K Deposited 2023-05-03 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 3 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Proteinase K was dissolved at pH 7.5 to 30 mg/mL in a 50 mM TRIS buffer . The protein was crystallized using a hanging drop setup on a 24 well VDX plate with sealant and 22 mm thick siliconized circle cover slides by mixing equal amounts of protein solution with ammonium sulfate.
|
Resolution 1.21 Å R-free 0.209 |
| 8SQV Proteinase K Multiconformer Model at 333K Deposited 2023-05-04 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | SO4 SULFATE ION × 4 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Proteinase K was dissolved at pH 7.5 to 30 mg/mL in a 50 mM TRIS buffer . The protein was crystallized using a hanging drop setup on a 24 well VDX plate with sealant and 22 mm thick siliconized circle cover slides by mixing equal amounts of protein solution with ammonium sulfate.
|
Resolution 1.22 Å R-free 0.169 |
| 9DHO Structure of proteinase K from energy-filtered MicroED data Deposited 2024-09-04 | Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 NO3 NITRATE ION × 1 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.09 Å R-free 0.183 |
| 9FTX Serial microseconds crystallography at ID29 using fixed-target (small foils): Proteinase K with 10 um spacing between X-ray pulses Deposited 2024-06-25 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；293 K；0.1 M MES pH 6.5, 0.5 M sodium nitrate, 0.1 M calcium chloride
|
Resolution 2.00 Å R-free 0.262 |
| 9FTY Serial microseconds crystallography at ID29 using fixed-target (small foils): Proteinase K with 20 um spacing between X-ray pulses Deposited 2024-06-25 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；293 K；0.1 M MES pH 6.5, 0.5 M sodium nitrate, 0.1 M calcium chloride
|
Resolution 2.00 Å R-free 0.262 |
| 9FU1 Serial microseconds crystallography at ID29 using fixed-target (large foils): Proteinase K with 50 um spacing between X-ray pulses Deposited 2024-06-25 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；293 K；0.1 M MES pH 6.5, 0.5 M sodium nitrate, 0.1 M calcium chloride
|
Resolution 2.00 Å R-free 0.224 |
| 9KW1 Crystal structure of proteinase K from Engyodontium album Deposited 2024-12-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | EU EUROPIUM ION × 2 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；293 K；0.5 M NaNO3, 0.1 M CaCl2, 0.1 M MES
|
Resolution 1.20 Å R-free 0.182 |
| 9ODV MicroED structure of proteinase K without energy filtering Deposited 2025-04-28 | Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 NO3 NITRATE ION × 1 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.30 Å R-free 0.226 |
| 9ODW MicroED structure of proteinase K with energy filtering Deposited 2025-04-28 | Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–384（279 aa）
|
Not recorded | CA CALCIUM ION × 2 NO3 NITRATE ION × 1 |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.30 Å R-free 0.228 |
256 other PDB entries and 256 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | PRTK_PARAQ |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–279； UniProt 106–384 |
