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6mky

Human SDS22

Method: X-RAY DIFFRACTION Dmax: 79.8 Å Quality: EXCELLENT
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Protein phosphatase 1 regulatory subunit 7

Homo sapiens

UniProt Q15435

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 57–317 Not recorded SO4 SULFATE ION × 8 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；298 K；0.2 M Ammonium sulfate, 0.01 M Cadmium chloride hemi(pentahydrate), 0.1 M HEPES 7.5, 25 % v/v PEG Smear Medium (12.5%w/v PEG 3350 12.5%w/v PEG 4000 12.5%w/v PEG 2000 12.5%w/v PEG 5000 MME) Resolution 2.90 Å R-free 0.272
2 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain B； UniProt 57–317 Not recorded SO4 SULFATE ION × 2 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；298 K；0.2 M Ammonium sulfate, 0.01 M Cadmium chloride hemi(pentahydrate), 0.1 M HEPES 7.5, 25 % v/v PEG Smear Medium (12.5%w/v PEG 3350 12.5%w/v PEG 4000 12.5%w/v PEG 2000 12.5%w/v PEG 5000 MME) Resolution 2.90 Å R-free 0.272

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 6MKY Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6HKW Crystal structure of human SDS22 Deposited 2018-09-09 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–360（360 aa）
Not recorded SO4 SULFATE ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；ammonium sulfate, PEG 4000, glycerol
Resolution 3.09 Å R-free 0.239
6HKW Crystal structure of human SDS22 Deposited 2018-09-09 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–360（360 aa）
Not recorded SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；ammonium sulfate, PEG 4000, glycerol
Resolution 3.09 Å R-free 0.239
6HKW Crystal structure of human SDS22 Deposited 2018-09-09 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 1–360（360 aa）
Not recorded SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；ammonium sulfate, PEG 4000, glycerol
Resolution 3.09 Å R-free 0.239
6HKW Crystal structure of human SDS22 Deposited 2018-09-09 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 1–360（360 aa）
Not recorded SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；ammonium sulfate, PEG 4000, glycerol
Resolution 3.09 Å R-free 0.239
6HKW Crystal structure of human SDS22 Deposited 2018-09-09 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain E 1–360（360 aa）
Not recorded SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；ammonium sulfate, PEG 4000, glycerol
Resolution 3.09 Å R-free 0.239
6OBN The crystal structure of coexpressed SDS22:PP1 complex Deposited 2019-03-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 56–360（305 aa） Fragment：UNP residues 56-360
Not recorded PO4 PHOSPHATE ION × 5 FE FE (III) ION × 1 CL CHLORIDE ION × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.2；289 K；14% PEG4000, 6% MPD, sodium/potassium phosphate
Resolution 2.70 Å R-free 0.246
6OBN The crystal structure of coexpressed SDS22:PP1 complex Deposited 2019-03-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 56–360（305 aa） Fragment：UNP residues 56-360
Not recorded PO4 PHOSPHATE ION × 3 FE FE (III) ION × 1 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.2；289 K；14% PEG4000, 6% MPD, sodium/potassium phosphate
Resolution 2.70 Å R-free 0.246
6OBP Reconstituted PP1 holoenzyme Deposited 2019-03-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 56–360（305 aa） Fragment：UNP residues 56-360
Not recorded PO4 PHOSPHATE ION × 2 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.2；289 K；14% PEG4000, 6% MPD, sodium/potassium phosphate
Resolution 2.70 Å R-free 0.258
8B5R p97-p37-SPI substrate complex Deposited 2022-09-24 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric
Chain S 1–360（360 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.10 Å
8U5G Crystal structure of the co-expressed SDS22:PP1:I3 complex Deposited 2023-09-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 56–360（305 aa）
Not recorded FE FE (III) ION × 1 PO4 PHOSPHATE ION × 10 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.2；289 K；1.8 M sodium phosphate monobasic monohydrate, potassium phosphate dibasic pH 8.2
Resolution 3.20 Å R-free 0.240

5 other PDB entries and 10 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PP1R7_HUMAN
Isoform Q15435-2
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–261； UniProt 57–317 Author chain B； PDBConstruct 1–261； UniProt 57–317

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 6mky

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 6mky
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2. Structure Basics 2. Structure Basics

Entry ID entry_id6mky
Deposition date deposition_date2018-09-26
Structure title titleHuman SDS22
Keywords keywordsSDS22, PP1, LRR repeat, cell cycle, SIGNALING PROTEIN; SIGNALING PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier26.15 Å
Radius of gyration Rg (electron density) rg_electron25.18 Å
Forward intensity I(0) i062320300.00
Molecular weight molecular_weight61229.0 kDa
Excluded volume excluded_volume76751 ų
Envelope volume envelope_volume93372 ų
Hydration-shell volume shell_volume30706 ų
Envelope diameter envelope_diameter82.5 Å
Shell Rg shell_rg32.66 Å
Envelope Rg envelope_rg25.28 Å
Shape Rg shape_rg25.18 Å
Total Rg total_rg25.99 Å
Total atoms total_atoms4281
Residues n_residues519
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax79.8 Å
Rg (real space) rg_real26.03 Å
Rg uncertainty (real space) rg_real_error0.38 Å
I(0) (real space) i0_real6.2320e+07
I(0) uncertainty (real space) i0_real_error8.8080e+05
Rg (reciprocal space) rg_reciprocal26.07 Å
I(0) (reciprocal space) i0_reciprocal62320000.0000
Solution quality estimate total_estimate0.9106
Solution quality rating solution_quality EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary31.4 Å
Skewness Skewness skewness0.166
Kurtosis Kurtosis kurtosis-0.529
Angular range angular_range— – 0.3050 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha20220000.0000
Real-space data points n_real_points62
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.950; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.995; Smooth: 0.989
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (3)

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8. Citations (1)

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9. Files and Curves (10)