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6olu

RIAM RA-PH core structure in the P212121 space group

Method: X-RAY DIFFRACTION Dmax: 63.8 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Amyloid beta A4 precursor protein-binding family B member 1-interacting protein

Mus musculus

UniProt Q8R5A3

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 179–437 Not recorded No other associated polymer X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；277 K；8% v/v Tacsimate pH 6, 20% w/v PEG3350 Resolution 1.90 Å R-free 0.256

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 6OLU Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3TCA Crystal structure of the Ras-associating and pleckstrin-homology domains of RIAM Deposited 2011-08-08 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 150–437（288 aa） Fragment：Ras-associating and PH domain, UNP residues 150-437
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；12% PEG3350, 100 mM LiAc, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.35 Å R-free 0.282
3TCA Crystal structure of the Ras-associating and pleckstrin-homology domains of RIAM Deposited 2011-08-08 Different construct Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 150–437（288 aa） Fragment：Ras-associating and PH domain, UNP residues 150-437
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；12% PEG3350, 100 mM LiAc, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.35 Å R-free 0.282
4KVG Crystal structure of RIAM RA-PH domains in complex with GTP bound Rap1 Deposited 2013-05-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 179–437（259 aa） Fragment：RA-PH domains (UNP residues 179-437)
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；0.1M ammonium sulfate, 0.1M Tris, 15% PEG 1500, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.65 Å R-free 0.219
4KVG Crystal structure of RIAM RA-PH domains in complex with GTP bound Rap1 Deposited 2013-05-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 179–437（259 aa） Fragment：RA-PH domains (UNP residues 179-437)
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；0.1M ammonium sulfate, 0.1M Tris, 15% PEG 1500, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.65 Å R-free 0.219
4W8P Crystal structure of RIAM TBS1 in complex with talin R7R8 domains Deposited 2014-08-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 5–25（21 aa）
Not recorded EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；100 mM NaCl, 20% (w/v) polyethylene glycol 3350 and 20% (v/v) ethylene glycol
Resolution 1.50 Å R-free 0.242
4W8P Crystal structure of RIAM TBS1 in complex with talin R7R8 domains Deposited 2014-08-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 5–25（21 aa）
Not recorded EDO 1,2-ETHANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；100 mM NaCl, 20% (w/v) polyethylene glycol 3350 and 20% (v/v) ethylene glycol
Resolution 1.50 Å R-free 0.242
6O6H RIAM cc-RA-PH structure in the P21212 space group Deposited 2019-03-06 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 150–437（288 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；100 mM sodium malonate pH 6.0, 16% PEG 3350, 3% 1,4-dioxane
Resolution 2.50 Å R-free 0.281

4 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name AB1IP_MOUSE
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 4–262； UniProt 179–437

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 6olu

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 6olu
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2. Structure Basics 2. Structure Basics

Entry ID entry_id6olu
Deposition date deposition_date2019-04-17
Structure title titleRIAM RA-PH core structure in the P212121 space group
Keywords keywordsRAP1 Effector RA-PH, SIGNALING PROTEIN; SIGNALING PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier19.95 Å
Radius of gyration Rg (electron density) rg_electron18.70 Å
Forward intensity I(0) i014342000.00
Molecular weight molecular_weight28733.0 kDa
Excluded volume excluded_volume36077 ų
Envelope volume envelope_volume42626 ų
Hydration-shell volume shell_volume19039 ų
Envelope diameter envelope_diameter65.7 Å
Shell Rg shell_rg24.93 Å
Envelope Rg envelope_rg19.06 Å
Shape Rg shape_rg18.65 Å
Total Rg total_rg19.77 Å
Total atoms total_atoms2021
Residues n_residues244
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax63.8 Å
Rg (real space) rg_real19.84 Å
Rg uncertainty (real space) rg_real_error0.42 Å
I(0) (real space) i0_real1.4340e+07
I(0) uncertainty (real space) i0_real_error1.7790e+05
Rg (reciprocal space) rg_reciprocal19.86 Å
I(0) (reciprocal space) i0_reciprocal14340000.0000
Solution quality estimate total_estimate0.8157
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks3
Primary peak position r_peak_primary25.1 Å
Skewness Skewness skewness0.191
Kurtosis Kurtosis kurtosis-0.382
Angular range angular_range— – 0.4000 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha2607000.0000
Real-space data points n_real_points72
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.867; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (2)

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7. Fold Classification (SCOP + CATH) 1 domains

CATH v4.4 (1 domains)

Domain ID domain_id6oluA01
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily90 — Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
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8. Citations (1)

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9. Files and Curves (10)