6tqs

The crystal structure of the MSP domain of human MOSPD2 in complex with the conventional FFAT motif of ORP1.

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Motile sperm domain-containing protein 2

Homo sapiens

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 2	Oxysterol-binding protein-related protein 1 × 1 （Q9BXW6） GLYCEROL × 3 trifluoroacetic acid × 1 SULFATE ION × 1 CHLORIDE ION × 1 water × 1	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 2	Oxysterol-binding protein-related protein 1 × 1 （Q9BXW6） GLYCEROL × 1 trifluoroacetic acid × 1 water × 1	Consistent with protein count
3	Protein heterocomplex	Heteromer	Protein 2	Oxysterol-binding protein-related protein 1 × 1 （Q9BXW6） GLYCEROL × 1 trifluoroacetic acid × 1 SULFATE ION × 1 DI(HYDROXYETHYL)ETHER × 1 water × 2	Consistent with protein count
4	Protein heterocomplex	Heteromer	Protein 2	Oxysterol-binding protein-related protein 1 × 1 （Q9BXW6） trifluoroacetic acid × 1 water × 1	Consistent with protein count
5	Protein heterocomplex	Heteromer	Protein 2	Oxysterol-binding protein-related protein 1 × 1 （Q9BXW6） trifluoroacetic acid × 1 SULFATE ION × 2 water × 2	Consistent with protein count
6	Protein monomer	Monomer	Protein 1	trifluoroacetic acid × 1 SULFATE ION × 1 PENTAETHYLENE GLYCOL × 1 water × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MSPD2_HUMAN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 2–210； UniProt 282–490 Author chain B； PDBConstruct 2–210； UniProt 282–490 Author chain C； PDBConstruct 2–210； UniProt 282–490 Author chain D； PDBConstruct 2–210； UniProt 282–490 Author chain E； PDBConstruct 2–210； UniProt 282–490 Author chain F； PDBConstruct 2–210； UniProt 282–490

Oxysterol-binding protein-related protein 1

OrganismNot specified

UniProt Q9BXW6

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 2	Motile sperm domain-containing protein 2 × 1 （Q8NHP6） GLYCEROL × 3 trifluoroacetic acid × 1 SULFATE ION × 1 CHLORIDE ION × 1 water × 1	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 2	Motile sperm domain-containing protein 2 × 1 （Q8NHP6） GLYCEROL × 1 trifluoroacetic acid × 1 water × 1	Consistent with protein count
3	Protein heterocomplex	Heteromer	Protein 2	Motile sperm domain-containing protein 2 × 1 （Q8NHP6） GLYCEROL × 1 trifluoroacetic acid × 1 SULFATE ION × 1 DI(HYDROXYETHYL)ETHER × 1 water × 2	Consistent with protein count
4	Protein heterocomplex	Heteromer	Protein 2	Motile sperm domain-containing protein 2 × 1 （Q8NHP6） trifluoroacetic acid × 1 water × 1	Consistent with protein count
5	Protein heterocomplex	Heteromer	Protein 2	Motile sperm domain-containing protein 2 × 1 （Q8NHP6） trifluoroacetic acid × 1 SULFATE ION × 2 water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	OSBL1_HUMAN
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain G； PDBConstruct 5–21； UniProt 469–485 Author chain H； PDBConstruct 5–21； UniProt 469–485 Author chain I； PDBConstruct 5–21； UniProt 469–485 Author chain J； PDBConstruct 5–21； UniProt 469–485 Author chain K； PDBConstruct 5–21； UniProt 469–485

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	6tqs
Deposition date deposition_date	2019-12-17
Structure title title	The crystal structure of the MSP domain of human MOSPD2 in complex with the conventional FFAT motif of ORP1.
Keywords keywords	Membrane contact sites, FFAT motif, MSP domain, Endoplasmic reticulum, PROTEIN BINDING; PROTEIN BINDING
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

6tqs__assembly_5__model_1

Assembly 5 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

6tqs__assembly_5__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

6tqs__assembly_5__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	16.44 Å
Rg (electron density)	15.06 Å
Total Rg	16.17 Å
Atom count	1061
Residues	136
Excluded volume	18815 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`6tqs__assembly_1__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`6tqs__assembly_2__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
3	1	`6tqs__assembly_3__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
4	1	`6tqs__assembly_4__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
5	1	`6tqs__assembly_5__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
6	1	`6tqs__assembly_6__model_1`	monomeric （1）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (9)

Entity 1 Entity 1 polymer

Description description	Motile sperm domain-containing protein 2
Molecular weight formula_weight	24268.6 kDa
Source method src_method	man
Copy count entity_count	6

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E,F
Atom count atom_count	6821

MISNEEQTPLLKKINPTESTSKAEENEKVDSKVKAFKKPLSVFKGPLLHISPAEELYFGSTESGEKKTLIVLTNVTKNIVAFKVRTTAPEKYRVKPSNSSCDPGASVDIVVSPHGGLTVSAQDRFLIMAAEMEQSSGTGPAELTQFWKEVPRNKVMEHRLRCHTVESSKPNTLTLKDNAFNMSDKTSEDICLQLSRLLESNRKLEDQVQRHHHHHH

Source Source

Source organism organism	Homo sapiens
Common name common_name	Human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Escherichia coli BL21(DE3)

Entity 2 Entity 2 polymer

Description description	Oxysterol-binding protein-related protein 1
Molecular weight formula_weight	2323.4 kDa
Source method src_method	syn
Copy count entity_count	5

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G,H,I,J,K

GAMRSILSEDEFYDALSDSES

Entity 3 Entity 3 non-polymer

Description description	GLYCEROL
Molecular weight formula_weight	92.1 kDa
Source method src_method	syn
Copy count entity_count	5

Entity 4 Entity 4 non-polymer

Description description	trifluoroacetic acid
Molecular weight formula_weight	114.0 kDa
Source method src_method	syn
Copy count entity_count	6

Entity 5 Entity 5 non-polymer

Description description	SULFATE ION
Molecular weight formula_weight	96.1 kDa
Source method src_method	syn
Copy count entity_count	5

Entity 6 Entity 6 non-polymer

Description description	CHLORIDE ION
Molecular weight formula_weight	35.5 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 7 Entity 7 non-polymer

Description description	DI(HYDROXYETHYL)ETHER
Molecular weight formula_weight	106.1 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 8 Entity 8 non-polymer

Description description	PENTAETHYLENE GLYCOL
Molecular weight formula_weight	238.3 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 9 Entity 9 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	270

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Unit-cell length a length_a	126.874 Å
Unit-cell length b length_b	126.874 Å
Unit-cell length c length_c	184.449 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	120.00 °
Molecules per unit cell Z z_pdb	72

Space group space_group	P 61 2 2
Space-group number int_tables_number	178
Crystal system crystal_system	—

Resolution resolution	2.250 Å
Low-resolution limit resolution_low	70.640 Å
R factor r_factor	0.2190
R_work r_work	0.2169
R_free r_free	0.2600

The crystal structure of the MSP domain of human MOSPD2 in complex with the conventional FFAT motif of ORP1.

1. Protein Identity and Related Structures Protein Identity & Related Structures

Motile sperm domain-containing protein 2

State in the Current Structure

Other States of the Same Protein in the Database

Oxysterol-binding protein-related protein 1

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

6tqs__assembly_5__model_1

6tqs__assembly_5__model_1 | I(q)

6tqs__assembly_5__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (9)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 non-polymer

Entity 7 Entity 7 non-polymer

Entity 8 Entity 8 non-polymer

Entity 9 Entity 9 water

7. Citations (1)

The crystal structure of the MSP domain of human MOSPD2 in complex with the conventional FFAT motif of ORP1.

1. Protein Identity and Related Structures Protein Identity & Related Structures

Motile sperm domain-containing protein 2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Oxysterol-binding protein-related protein 1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

6tqs__assembly_5__model_1

6tqs__assembly_5__model_1 | I(q)

6tqs__assembly_5__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (9)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 non-polymer

Entity 7 Entity 7 non-polymer

Entity 8 Entity 8 non-polymer

Entity 9 Entity 9 water

7. Citations (1)