SAXSdb — 7qgk

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1. Protein Identity and Related Structures Protein Identity & Related Structures

The red form of the mRubyFT protein, Genetically Encoded Blue-to-Red Fluorescent Timer

Entacmaea quadricolor

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein monomer	Monomer	Protein 1	MAGNESIUM ION × 3 water × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RFP_ENTQU
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 13–228； UniProt 4–221

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	7qgk
Deposition date deposition_date	2021-12-08
Structure title title	The mRubyFT protein, Genetically Encoded Blue-to-Red Fluorescent Timer in its red state
Keywords keywords	fluorescent protein, flourescent timer, mRubyFT, blue to red timer, chromophore; FLUORESCENT PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

7qgk__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

7qgk__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

7qgk__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	17.68 Å
Rg (electron density)	16.55 Å
Total Rg	17.62 Å
Atom count	1721
Residues	217
Excluded volume	30570 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`7qgk__assembly_1__model_1`	monomeric （1）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Description description	The red form of the mRubyFT protein, Genetically Encoded Blue-to-Red Fluorescent Timer
Molecular weight formula_weight	27015.9 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	1990

GHMRSMVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMRIKVIEGGPLPFAFDILATSFLYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVVTVMQDTSLEDGCLVYHVQVRGVDFPSNGPVMQKKTKGWEPSTEMMYPADGGLRGYTHMALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVLREHSVAKFAGRGGMDELYK

Source Source

Source organism organism	Entacmaea quadricolor
NCBI taxonomy ID ncbi_taxonomy_id	6118
Host organism host_organism	Escherichia coli

Entity 2 Entity 2 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	3

Entity 3 Entity 3 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	156

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Unit-cell length a length_a	31.338 Å
Unit-cell length b length_b	66.253 Å
Unit-cell length c length_c	96.496 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	4

Space group space_group	P 21 21 21
Space-group number int_tables_number	19
Crystal system crystal_system	—

Resolution resolution	1.500 Å
Low-resolution limit resolution_low	54.620 Å
R factor r_factor	0.1748
R_work r_work	0.1738
R_free r_free	0.1949

The mRubyFT protein, Genetically Encoded Blue-to-Red Fluorescent Timer in its red state

1. Protein Identity and Related Structures Protein Identity & Related Structures

The red form of the mRubyFT protein, Genetically Encoded Blue-to-Red Fluorescent Timer

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

7qgk__assembly_1__model_1

7qgk__assembly_1__model_1 | I(q)

7qgk__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 water

7. Citations (1)

The mRubyFT protein, Genetically Encoded Blue-to-Red Fluorescent Timer in its red state

1. Protein Identity and Related Structures Protein Identity & Related Structures

The red form of the mRubyFT protein, Genetically Encoded Blue-to-Red Fluorescent Timer

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

7qgk__assembly_1__model_1

7qgk__assembly_1__model_1 | I(q)

7qgk__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 water

7. Citations (1)