7wga

2.2 ANGSTROMS RESOLUTION STRUCTURE ANALYSIS OF TWO REFINED N-ACETYLNEURAMINYLLACTOSE-WHEAT GERM AGGLUTININ ISOLECTIN COMPLEXES

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

WHEAT GERM LECTIN

Triticum aestivum

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 2	water × 2	Consistent with protein count
2	Protein monomer	Monomer	Protein 1	water × 1	Consistent with protein count
3	Protein homooligomer	Homooligomer	Protein 2	water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	AGI1_WHEAT
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 2–171； UniProt 28–197 Author chain B； PDBConstruct 2–171； UniProt 28–197

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	7wga
Deposition date deposition_date	1990-04-03
Structure title title	2.2 ANGSTROMS RESOLUTION STRUCTURE ANALYSIS OF TWO REFINED N-ACETYLNEURAMINYLLACTOSE-WHEAT GERM AGGLUTININ ISOLECTIN COMPLEXES
Keywords keywords	LECTIN (AGGLUTININ); LECTIN (AGGLUTININ)
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

7wga__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

7wga__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

7wga__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	20.65 Å
Rg (electron density)	20.05 Å
Total Rg	20.59 Å
Atom count	2300
Residues	340
Excluded volume	39054 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`7wga__assembly_1__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`7wga__assembly_2__model_1`	monomeric （1）	Success	4.1.3-1-20251215 (887e7ef)	View Download
3	1	`7wga__assembly_3__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (2)

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6. Fold Classification (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

Domain ID domain_id	d7wgaa1
Class class	g — Small proteins
Fold Fold fold	g.3 — Knottins (small inhibitors, toxins, lectins)
Superfamily Superfamily superfamily	g.3.1 — Plant lectins/antimicrobial peptides
Family Family family	g.3.1.1 — Hevein-like agglutinin (lectin) domain

Domain ID domain_id	d7wgaa2
Class class	g — Small proteins
Fold Fold fold	g.3 — Knottins (small inhibitors, toxins, lectins)
Superfamily Superfamily superfamily	g.3.1 — Plant lectins/antimicrobial peptides
Family Family family	g.3.1.1 — Hevein-like agglutinin (lectin) domain

Domain ID domain_id	d7wgaa3
Class class	g — Small proteins
Fold Fold fold	g.3 — Knottins (small inhibitors, toxins, lectins)
Superfamily Superfamily superfamily	g.3.1 — Plant lectins/antimicrobial peptides
Family Family family	g.3.1.1 — Hevein-like agglutinin (lectin) domain

Domain ID domain_id	d7wgaa4
Class class	g — Small proteins
Fold Fold fold	g.3 — Knottins (small inhibitors, toxins, lectins)
Superfamily Superfamily superfamily	g.3.1 — Plant lectins/antimicrobial peptides
Family Family family	g.3.1.1 — Hevein-like agglutinin (lectin) domain

Domain ID domain_id	d7wgab1
Class class	g — Small proteins
Fold Fold fold	g.3 — Knottins (small inhibitors, toxins, lectins)
Superfamily Superfamily superfamily	g.3.1 — Plant lectins/antimicrobial peptides
Family Family family	g.3.1.1 — Hevein-like agglutinin (lectin) domain

Domain ID domain_id	d7wgab2
Class class	g — Small proteins
Fold Fold fold	g.3 — Knottins (small inhibitors, toxins, lectins)
Superfamily Superfamily superfamily	g.3.1 — Plant lectins/antimicrobial peptides
Family Family family	g.3.1.1 — Hevein-like agglutinin (lectin) domain

Domain ID domain_id	d7wgab3
Class class	g — Small proteins
Fold Fold fold	g.3 — Knottins (small inhibitors, toxins, lectins)
Superfamily Superfamily superfamily	g.3.1 — Plant lectins/antimicrobial peptides
Family Family family	g.3.1.1 — Hevein-like agglutinin (lectin) domain

Domain ID domain_id	d7wgab4
Class class	g — Small proteins
Fold Fold fold	g.3 — Knottins (small inhibitors, toxins, lectins)
Superfamily Superfamily superfamily	g.3.1 — Plant lectins/antimicrobial peptides
Family Family family	g.3.1.1 — Hevein-like agglutinin (lectin) domain

CATH v4.4 (8 domains)

Domain ID domain_id	7wgaA01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	60 — Wheat Germ Agglutinin (Isolectin 2); domain 1
Homologous superfamily homologous superfamily	10 — Endochitinase-like

Domain ID domain_id	7wgaA02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	60 — Wheat Germ Agglutinin (Isolectin 2); domain 1
Homologous superfamily homologous superfamily	10 — Endochitinase-like

Domain ID domain_id	7wgaA03
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	60 — Wheat Germ Agglutinin (Isolectin 2); domain 1
Homologous superfamily homologous superfamily	10 — Endochitinase-like

Domain ID domain_id	7wgaA04
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	60 — Wheat Germ Agglutinin (Isolectin 2); domain 1
Homologous superfamily homologous superfamily	10 — Endochitinase-like

Domain ID domain_id	7wgaB01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	60 — Wheat Germ Agglutinin (Isolectin 2); domain 1
Homologous superfamily homologous superfamily	10 — Endochitinase-like

Domain ID domain_id	7wgaB02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	60 — Wheat Germ Agglutinin (Isolectin 2); domain 1
Homologous superfamily homologous superfamily	10 — Endochitinase-like

Domain ID domain_id	7wgaB03
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	60 — Wheat Germ Agglutinin (Isolectin 2); domain 1
Homologous superfamily homologous superfamily	10 — Endochitinase-like

Domain ID domain_id	7wgaB04
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	60 — Wheat Germ Agglutinin (Isolectin 2); domain 1
Homologous superfamily homologous superfamily	10 — Endochitinase-like

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7. Citations (18)

2.2 A resolution structure analysis of two refined N-acetylneuraminyl-lactose--wheat germ agglutinin isolectin complexes.

J.Mol.Biol. (1990)

PMID: 2231724 · DOI: 10.1016/S0022-2836(05)80174-3 PRIMARY

Comparison of the Refined Crystal Structures of Two Wheat Germ Isolectins

J.Mol.Biol. (1989)

Sequence Variability in Three Wheat Germ Agglutinin Isolectins. Products of Multiple Genes in Polyploid Wheat

J.Mol.Evol. (1989)

Erratum. Refinement of the Crystal Structure of Wheat Germ Agglutinin Isolectin 2 at 1.8 Angstroms Resolution

J.Mol.Biol. (1988)

Refinement of the Crystal Structure of Wheat Germ Agglutinin Isolectin 2 at 1.8 Angstroms Resolution

J.Mol.Biol. (1987)

;Preliminary X-Ray Diffraction Results on Co-Crystals of Wheat Germ Agglutinin with a Sialoglycopeptide from the Red Cell Receptor Glycophorina ;

J.Mol.Biol. (1987)

Structural Differences in the Two Major Wheat Germ Agglutinin Isolectins

J.Biol.Chem. (1986)

Evolution of the Multidomain Protein Wheat Germ Agglutinin

J.Mol.Evol. (1985)

Structural Comparison of the Two Distinct Sugar Binding Sites in Wheat Germ Agglutinin Isolectin II

J.Mol.Biol. (1984)

Primary Structure of Wheat Germ Agglutinin Isolectin 2. Peptide Order Deduced from X-Ray Structure

Biochemistry (1984)

Histidine Determination in Wheat Germ Agglutinin Isolectin by X-Ray Diffraction Analysis

J.Mol.Biol. (1981)

Multi-Domain Structure of the Dimeric Lectin Wheat Germ Agglutinin

Biomolecular Structure, Conformation, Function and Evolution (1980)

;Location of the N-Acetyl-D-Neuraminic Acid Binding Site in Wheat Germ Agglutinin. A Crystallographic Study at 2.8 Angstroms Resolution ;

J.Mol.Biol. (1980)

The Toxin-Agglutinin Fold. A New Group of Small Protein Structures Organized Around a Four-Disulfide Core

J.Biol.Chem. (1980)

Crystallographic Elucidation of the Saccharide Binding Mode in Wheat Germ Agglutinin and its Biological Significance

J.Mol.Biol. (1980)

The Crystal Structure of Wheat Germ Agglutinin at 2.2 Angstroms Resolution

J.Mol.Biol. (1977)

Non-Crystallographic Symmetry in the Crystal Dimer of Wheat Germ Agglutinin

J.Mol.Biol. (1974)

A Preliminary Crystallographic Study of Wheat Germ Agglutinin

J.Mol.Biol. (1974)

Unit-cell length a length_a	51.220 Å
Unit-cell length b length_b	73.600 Å
Unit-cell length c length_c	91.420 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	98.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	8

Space group space_group	C 1 2 1
Space-group number int_tables_number	5
Crystal system crystal_system	—

Resolution resolution	2.000 Å
Low-resolution limit resolution_low	8.000 Å
R factor r_factor	0.1720
R_work r_work	—
R_free r_free	—

Description description	WHEAT GERM LECTIN
Molecular weight formula_weight	17124.1 kDa
Source method src_method	man
Copy count entity_count	2

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B
Atom count atom_count	2530

2.2 ANGSTROMS RESOLUTION STRUCTURE ANALYSIS OF TWO REFINED N-ACETYLNEURAMINYLLACTOSE-WHEAT GERM AGGLUTININ ISOLECTIN COMPLEXES

1. Protein Identity and Related Structures Protein Identity & Related Structures

WHEAT GERM LECTIN

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

7wga__assembly_1__model_1

7wga__assembly_1__model_1 | I(q)

7wga__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 water

6. Fold Classification (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

CATH v4.4 (8 domains)

7. Citations (18)

Source organism organism	Triticum aestivum
Common name common_name	bread wheat
NCBI taxonomy ID ncbi_taxonomy_id	4565

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	217

2.2 ANGSTROMS RESOLUTION STRUCTURE ANALYSIS OF TWO REFINED N-ACETYLNEURAMINYLLACTOSE-WHEAT GERM AGGLUTININ ISOLECTIN COMPLEXES

1. Protein Identity and Related Structures Protein Identity & Related Structures

WHEAT GERM LECTIN

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

7wga__assembly_1__model_1

7wga__assembly_1__model_1 | I(q)

7wga__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 water

6. Fold Classification (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

CATH v4.4 (8 domains)

7. Citations (18)