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7xno

Cryo-EM structure of the bacteriocin-receptor-immunity ternary complex from Lactobacillus sakei

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Bacteriocin sakacin-A

Latilactobacillus sakei L45

UniProt P0A310

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 12 Mannose permease IIC component × 3 （A0A094YUG1） Mannose permease IID component × 3 （A0A094XZA1） Sakacin-A immunity factor × 3 （Q48864） alpha-D-mannopyranose × 3 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
7xno

1 assemblies

View Construct and Data Evidence
UniProt name SAKA_LATSK
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–41； UniProt 19–59 Author chain C； PDBConstruct 1–41； UniProt 19–59 Author chain G； PDBConstruct 1–41； UniProt 19–59

Mannose permease IIC component

Latilactobacillus sakei L45

UniProt A0A094YUG1

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 12 Bacteriocin sakacin-A × 3 （P0A310） Mannose permease IID component × 3 （A0A094XZA1） Sakacin-A immunity factor × 3 （Q48864） alpha-D-mannopyranose × 3 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
7xno 7xtg

2 assemblies

View Construct and Data Evidence
UniProt name A0A094YUG1_LATSK
Isoform —
PDB entities 2
Chains and sequence ranges Author chain D； PDBConstruct 1–268； UniProt 1–268 Author chain H； PDBConstruct 1–268； UniProt 1–268 Author chain Y； PDBConstruct 1–268； UniProt 1–268

Mannose permease IID component

Latilactobacillus sakei L45

UniProt A0A094XZA1

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 12 Bacteriocin sakacin-A × 3 （P0A310） Mannose permease IIC component × 3 （A0A094YUG1） Sakacin-A immunity factor × 3 （Q48864） alpha-D-mannopyranose × 3 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
7xno 7xtg

2 assemblies

View Construct and Data Evidence
UniProt name A0A094XZA1_LATSK
Isoform —
PDB entities 3
Chains and sequence ranges Author chain E； PDBConstruct 1–303； UniProt 1–303 Author chain I； PDBConstruct 1–303； UniProt 1–303 Author chain Z； PDBConstruct 1–303； UniProt 1–303

Sakacin-A immunity factor

Latilactobacillus sakei L45

UniProt Q48864

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 12 Bacteriocin sakacin-A × 3 （P0A310） Mannose permease IIC component × 3 （A0A094YUG1） Mannose permease IID component × 3 （A0A094XZA1） alpha-D-mannopyranose × 3 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
7xno 7xtg

2 assemblies

View Construct and Data Evidence
UniProt name SAIA_LATSK
Isoform —
PDB entities 4
Chains and sequence ranges Author chain B； PDBConstruct 30–119； UniProt 1–90 Author chain F； PDBConstruct 30–119； UniProt 1–90 Author chain J； PDBConstruct 30–119； UniProt 1–90

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id7xno
Deposition date deposition_date2022-04-29
Structure title titleCryo-EM structure of the bacteriocin-receptor-immunity ternary complex from Lactobacillus sakei
Keywords keywords;antimicrobial peptides, bacteriocins, pediocin-like/class IIa bacteriocins, antibiotic resistance, mannose phosphotransferase, man-PTS, immunity, self-protection, MEMBRANE PROTEIN, MEMBRANE PROTEIN-IMMUNE SYSTEM complex ;; MEMBRANE PROTEIN/IMMUNE SYSTEM
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

7xno__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

7xno__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

7xno__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）43.04 Å
Rg (electron density)42.47 Å
Total Rg42.87 Å
Atom count15888
Residues2085
Excluded volume290710 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 7xno__assembly_1__model_1 dodecameric （12） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (5)

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7. Citations (1)