SAXSdb — 8a36

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Sensory box protein

Pseudomonas putida KT2440

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 2	FLAVIN MONONUCLEOTIDE × 2	Consistent with protein count
2	Protein homooligomer	Homooligomer	Protein 2	FLAVIN MONONUCLEOTIDE × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	Q88E39_PSEPK
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 21–162； UniProt 1–142 Author chain B； PDBConstruct 21–162； UniProt 1–142 Author chain C； PDBConstruct 21–162； UniProt 1–142 Author chain D； PDBConstruct 21–162； UniProt 1–142

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	8a36
Deposition date deposition_date	2022-06-07
Structure title title	Crystal structure of PpSB1-LOV-K117E mutant (dark state), monoclinic form
Keywords keywords	LOV domain; short LOV; PAS domain; Photocycle; Dimerization; Signaling blue light photoreceptor, SIGNALING PROTEIN; SIGNALING PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8a36__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8a36__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

8a36__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	21.34 Å
Rg (electron density)	20.23 Å
Total Rg	21.07 Å
Atom count	2218
Residues	266
Excluded volume	38779 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`8a36__assembly_1__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`8a36__assembly_2__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (2)

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Unit-cell length a length_a	98.754 Å
Unit-cell length b length_b	80.929 Å
Unit-cell length c length_c	94.370 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	93.85 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	16

Space group space_group	C 1 2 1
Space-group number int_tables_number	5
Crystal system crystal_system	monoclinic

Resolution resolution	2.700 Å
Low-resolution limit resolution_low	42.500 Å
R factor r_factor	0.2273
R_work r_work	0.2252
R_free r_free	0.2718

Description description	Sensory box protein
Molecular weight formula_weight	18617.8 kDa
Mutation mutation	K117E
Source method src_method	man
Copy count entity_count	4

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D
Atom count atom_count	4431

Crystal structure of PpSB1-LOV-K117E mutant (dark state), monoclinic form

1. Protein Identity and Related Structures Protein Identity & Related Structures

Sensory box protein

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

8a36__assembly_1__model_1

8a36__assembly_1__model_1 | I(q)

8a36__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

7. Citations (1)

Source organism organism	Pseudomonas putida KT2440
NCBI taxonomy ID ncbi_taxonomy_id	160488
Host organism host_organism	;Escherichia coli 'BL21-Gold(DE3)pLysS AG' ;

Description description	FLAVIN MONONUCLEOTIDE
Molecular weight formula_weight	456.3 kDa
Source method src_method	syn
Copy count entity_count	4

Crystal structure of PpSB1-LOV-K117E mutant (dark state), monoclinic form

1. Protein Identity and Related Structures Protein Identity & Related Structures

Sensory box protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

8a36__assembly_1__model_1

8a36__assembly_1__model_1 | I(q)

8a36__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

7. Citations (1)