SAXSdb — 8asz

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Putrescine-binding periplasmic protein PotF

Escherichia coli K-12

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count	Chain A； UniProt 27–370	Mutation：S87Y, A182D	AG2 AGMATINE × 1 EDO 1,2-ETHANEDIOL × 10 GOL GLYCEROL × 2 PEG DI(HYDROXYETHYL)ETHER × 2 CA CALCIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 4.6；293 K；0.17 M AmAcetate, 0.085 M NaAcetate pH 4.6, 30% PEG 4000, 15% Glycerol	Resolution 1.28 Å R-free 0.209

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 8ASZ	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
1A99 PUTRESCINE RECEPTOR (POTF) FROM E. COLI Deposited 1998-04-17	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 27–370（344 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 5；PROTEIN WAS CRYSTALLIZED FROM 2M AMMONIUM SULFATE, 6% GLYCEROL, 200MM CACODYLATE BUFFER (PH 5), pH 5.0	Resolution 2.20 Å R-free 0.233
1A99 PUTRESCINE RECEPTOR (POTF) FROM E. COLI Deposited 1998-04-17	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 27–370（344 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 5；PROTEIN WAS CRYSTALLIZED FROM 2M AMMONIUM SULFATE, 6% GLYCEROL, 200MM CACODYLATE BUFFER (PH 5), pH 5.0	Resolution 2.20 Å R-free 0.233
1A99 PUTRESCINE RECEPTOR (POTF) FROM E. COLI Deposited 1998-04-17	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain C 27–370（344 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 5；PROTEIN WAS CRYSTALLIZED FROM 2M AMMONIUM SULFATE, 6% GLYCEROL, 200MM CACODYLATE BUFFER (PH 5), pH 5.0	Resolution 2.20 Å R-free 0.233
1A99 PUTRESCINE RECEPTOR (POTF) FROM E. COLI Deposited 1998-04-17	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain D 27–370（344 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 5；PROTEIN WAS CRYSTALLIZED FROM 2M AMMONIUM SULFATE, 6% GLYCEROL, 200MM CACODYLATE BUFFER (PH 5), pH 5.0	Resolution 2.20 Å R-free 0.233
6YE0 E.coli's Putrescine receptor PotF complexed with Putrescine Deposited 2020-03-23	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 27–370（344 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 1 JFN (2R)-1-methoxypropan-2-amine × 2 ONW (2~{R})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-amine × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.8；293 K；2.4 M Ammoniumsulfate, 0.1 M Bicine pH 8.8, 4.5% Jeffamine M600	Resolution 1.63 Å R-free 0.200
6YE0 E.coli's Putrescine receptor PotF complexed with Putrescine Deposited 2020-03-23	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 27–370（344 aa）	Not recorded	PUT 1,4-DIAMINOBUTANE × 1 JFN (2R)-1-methoxypropan-2-amine × 1 ONT (2~{S})-1-(2-methoxyethoxy)propan-2-amine × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.8；293 K；2.4 M Ammoniumsulfate, 0.1 M Bicine pH 8.8, 4.5% Jeffamine M600	Resolution 1.63 Å R-free 0.200
6YE6 E.coli's Putrescine receptor PotF complexed with Agmatine Deposited 2020-03-24	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 27–370（344 aa）	Not recorded	ONT (2~{S})-1-(2-methoxyethoxy)propan-2-amine × 2 JFN (2R)-1-methoxypropan-2-amine × 7 AG2 AGMATINE × 1 ONW (2~{R})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-amine × 1 SO4 SULFATE ION × 1 CL CHLORIDE ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.8；293 K；2.4 M Ammoniumsulfate, 0.1 M Bicine pH 8.8, 7% Jeffamine M600	Resolution 1.56 Å R-free 0.185
6YE6 E.coli's Putrescine receptor PotF complexed with Agmatine Deposited 2020-03-24	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 27–370（344 aa）	Not recorded	ONT (2~{S})-1-(2-methoxyethoxy)propan-2-amine × 2 JFN (2R)-1-methoxypropan-2-amine × 8 AG2 AGMATINE × 1 SO4 SULFATE ION × 3 CL CHLORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.8；293 K；2.4 M Ammoniumsulfate, 0.1 M Bicine pH 8.8, 7% Jeffamine M600	Resolution 1.56 Å R-free 0.185
6YE7 E.coli's Putrescine receptor PotF complexed with Cadaverine Deposited 2020-03-24	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 27–370（344 aa）	Not recorded	N2P PENTANE-1,5-DIAMINE × 1 EDO 1,2-ETHANEDIOL × 12 PG4 TETRAETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 1 PGE TRIETHYLENE GLYCOL × 1 TAM TRIS(HYDROXYETHYL)AMINOMETHANE × 1 CL CHLORIDE ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.8；293 K；0.1 M Bicine pH 8.8, 2.4 M Ammoniumsulfate, 5% PEG 3550	Resolution 1.60 Å R-free 0.194
6YE7 E.coli's Putrescine receptor PotF complexed with Cadaverine Deposited 2020-03-24	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 27–370（344 aa）	Not recorded	N2P PENTANE-1,5-DIAMINE × 1 EDO 1,2-ETHANEDIOL × 11 PEG DI(HYDROXYETHYL)ETHER × 1 CL CHLORIDE ION × 1 P33 3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL × 1 MLI MALONATE ION × 1 1PE PENTAETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.8；293 K；0.1 M Bicine pH 8.8, 2.4 M Ammoniumsulfate, 5% PEG 3550	Resolution 1.60 Å R-free 0.194
6YE8 E.coli's Putrescine receptor PotF complexed with Spermidine Deposited 2020-03-24	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 27–370（344 aa）	Not recorded	SPD SPERMIDINE × 1 JFN (2R)-1-methoxypropan-2-amine × 3 ONT (2~{S})-1-(2-methoxyethoxy)propan-2-amine × 1 CL CHLORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.8；293 K；2.4 M Ammoniumsulfate, 0.1 M Bicine pH 8.8, 10% Jeffamine M600	Resolution 1.50 Å R-free 0.183
6YE8 E.coli's Putrescine receptor PotF complexed with Spermidine Deposited 2020-03-24	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 27–370（344 aa）	Not recorded	SPD SPERMIDINE × 1 JFN (2R)-1-methoxypropan-2-amine × 4 ONT (2~{S})-1-(2-methoxyethoxy)propan-2-amine × 1 CL CHLORIDE ION × 1 SO4 SULFATE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.8；293 K；2.4 M Ammoniumsulfate, 0.1 M Bicine pH 8.8, 10% Jeffamine M600	Resolution 1.50 Å R-free 0.183
6YEB E.coli's Putrescine receptor PotF in its closed apo state Deposited 2020-03-24	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 27–370（344 aa）	Not recorded	GOL GLYCEROL × 1 PGE TRIETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 1 EDO 1,2-ETHANEDIOL × 7 SO4 SULFATE ION × 2 CL CHLORIDE ION × 3	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.8；293 K；2.4 M Ammoniumsulfate, 0.1 M Bicine pH 8.8, 5% PEG 3550	Resolution 1.97 Å R-free 0.219
6YEB E.coli's Putrescine receptor PotF in its closed apo state Deposited 2020-03-24	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 27–370（344 aa）	Not recorded	PEG DI(HYDROXYETHYL)ETHER × 5 EDO 1,2-ETHANEDIOL × 10 SO4 SULFATE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.8；293 K；2.4 M Ammoniumsulfate, 0.1 M Bicine pH 8.8, 5% PEG 3550	Resolution 1.97 Å R-free 0.219
6YEC E.coli's Putrescine receptor PotF complexed with Spermine Deposited 2020-03-24	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 27–370（344 aa）	Not recorded	SPM SPERMINE × 1 EDO 1,2-ETHANEDIOL × 9 PEG DI(HYDROXYETHYL)ETHER × 1 PGE TRIETHYLENE GLYCOL × 1 SO4 SULFATE ION × 3 CL CHLORIDE ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.8；293 K；2.4 M Ammoniumsulfate, 0.1 M Bicine pH 8.8, 5% PEG 3550	Resolution 2.09 Å R-free 0.240
6YEC E.coli's Putrescine receptor PotF complexed with Spermine Deposited 2020-03-24	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 27–370（344 aa）	Not recorded	SPM SPERMINE × 1 EDO 1,2-ETHANEDIOL × 6 PEG DI(HYDROXYETHYL)ETHER × 1 PGE TRIETHYLENE GLYCOL × 1 SO4 SULFATE ION × 2 CL CHLORIDE ION × 3 GOL GLYCEROL × 1 PG4 TETRAETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.8；293 K；2.4 M Ammoniumsulfate, 0.1 M Bicine pH 8.8, 5% PEG 3550	Resolution 2.09 Å R-free 0.240
6YED E.coli's Putrescine receptor PotF in its open apo state Deposited 2020-03-24	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 27–370（344 aa）	Not recorded	PGE TRIETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 2 EDO 1,2-ETHANEDIOL × 11 CL CHLORIDE ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；293 K；0.1 M Tris pH 7.2, 0.2 M NaCl, 35% PEG 3000	Resolution 2.18 Å R-free 0.273
6YED E.coli's Putrescine receptor PotF in its open apo state Deposited 2020-03-24	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 27–370（344 aa）	Not recorded	PEG DI(HYDROXYETHYL)ETHER × 4 EDO 1,2-ETHANEDIOL × 4 CL CHLORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；293 K；0.1 M Tris pH 7.2, 0.2 M NaCl, 35% PEG 3000	Resolution 2.18 Å R-free 0.273
7OYT E.coli's putrescine receptor variant PotF/D (4JDF) with mutations E39D F88L in complex with spermidine Deposited 2021-06-25	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 27–370（344 aa）	Mutation：S38T, S87Y, F88L, A182D, D247S, F276W, L348Q	SPD SPERMIDINE × 1 GOL GLYCEROL × 2 ACT ACETATE ION × 1 EDO 1,2-ETHANEDIOL × 17 PEG DI(HYDROXYETHYL)ETHER × 2 PGE TRIETHYLENE GLYCOL × 1 CL CHLORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；293 K；0.1 M Sodium acetate pH 4.6, 0.2 M Ammonium acetate, 30% PEG 4000	Resolution 1.60 Å R-free 0.209
7OYU E.coli's putrescine receptor variant PotF/D (4JDF) with mutations E39D Y87S F88Y in complex with spermidine Deposited 2021-06-25	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 27–370（344 aa）	Mutation：S38T, F88Y, A182D, D247S, F276W, L348Q	PGE TRIETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 7 PEG DI(HYDROXYETHYL)ETHER × 1 SPD SPERMIDINE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；293 K；0.1 M Sodium acetate pH 4.6, 0.2 M Ammonium acetate, 30% PEG 4000	Resolution 1.95 Å R-free 0.242
7OYX E.coli's putrescine receptor variant PotF/D (4JDF) with mutations E39D Y87S F88Y S247D in complex with spermidine Deposited 2021-06-25	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 27–370（344 aa）	Mutation：S38T, F88Y, A182D, F276W, L348Q	SPD SPERMIDINE × 1 3IE (2~{S})-1-methoxypropan-2-amine × 2 4SW (2~{S})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-amine × 1 CL CHLORIDE ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.3；293 K；2.4 M AmSO4 + 0.1 M Bicine pH 8.3 + 4.5% Jeffamine M600	Resolution 1.37 Å R-free 0.193
7OYX E.coli's putrescine receptor variant PotF/D (4JDF) with mutations E39D Y87S F88Y S247D in complex with spermidine Deposited 2021-06-25	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 27–370（344 aa）	Mutation：S38T, F88Y, A182D, F276W, L348Q	SPD SPERMIDINE × 1 3IE (2~{S})-1-methoxypropan-2-amine × 3 CL CHLORIDE ION × 1 4RH (2~{R})-1-(2-methoxyethoxy)propan-2-amine × 1 3IK (2~{R})-1-[(2~{R})-1-[(2~{S})-1-[(2~{S})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-yl]oxypropan-2-yl]oxypropan-2-amine × 1 ONT (2~{S})-1-(2-methoxyethoxy)propan-2-amine × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.3；293 K；2.4 M AmSO4 + 0.1 M Bicine pH 8.3 + 4.5% Jeffamine M600	Resolution 1.37 Å R-free 0.193
7OYY E.coli's putrescine receptor variant PotF/D (4JDF) with mutation S247D in complex with spermidine Deposited 2021-06-25	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 27–370（344 aa）	Mutation：S38T, D39E, S87Y, A182D, F276W, L348Q	SPD SPERMIDINE × 1 GOL GLYCEROL × 1 EDO 1,2-ETHANEDIOL × 3 PEG DI(HYDROXYETHYL)ETHER × 1 CL CHLORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5；293 K；0.1 M MES pH 5, 30% PEG 6000	Resolution 1.36 Å R-free 0.186
7OYZ E.coli's putrescine receptor variant PotF/D in complex with spermidine Deposited 2021-06-25	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 27–370（344 aa）	Mutation：S38T, D39E, S87Y, A182D, D247S, F276W, L348Q	SPD SPERMIDINE × 1 EDO 1,2-ETHANEDIOL × 1 CL CHLORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；25% (w/v) PEG 3350, 0.1M Sodium Acetate, pH 4.5	Resolution 1.49 Å R-free 0.215
8AT0 PotF with mutation D247K Deposited 2022-08-22	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 29–370（342 aa）	Mutation：D247K	ONT (2~{S})-1-(2-methoxyethoxy)propan-2-amine × 2 SO4 SULFATE ION × 2 JFN (2R)-1-methoxypropan-2-amine × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 9；293 K；2.4 M AmSO4, 0.1 M Bicine pH 9, 10% Jeffamine M600 pH7.0	Resolution 2.00 Å R-free 0.243
8AT0 PotF with mutation D247K Deposited 2022-08-22	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 29–370（342 aa）	Mutation：D247K	ONT (2~{S})-1-(2-methoxyethoxy)propan-2-amine × 1 SO4 SULFATE ION × 2 JFN (2R)-1-methoxypropan-2-amine × 1 MLI MALONATE ION × 1 EDO 1,2-ETHANEDIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 9；293 K；2.4 M AmSO4, 0.1 M Bicine pH 9, 10% Jeffamine M600 pH7.0	Resolution 2.00 Å R-free 0.243

14 other PDB entries and 26 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	POTF_ECOLI
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–344； UniProt 27–370

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	8asz
Deposition date deposition_date	2022-08-22
Structure title title	PotF with mutations S87Y and A182D in complex with Agmatine
Keywords keywords	Periplasmic-binding protein, Agmatine, TRANSPORT PROTEIN; TRANSPORT PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	21.38 Å
Radius of gyration Rg (electron density) rg_electron	20.29 Å
Forward intensity I(0) i0	25005900.00
Molecular weight molecular_weight	39409.0 kDa
Excluded volume excluded_volume	49800 Å³
Envelope volume envelope_volume	56009 Å³
Hydration-shell volume shell_volume	22813 Å³
Envelope diameter envelope_diameter	73.8 Å
Shell Rg shell_rg	27.04 Å
Envelope Rg envelope_rg	20.56 Å
Shape Rg shape_rg	20.23 Å
Total Rg total_rg	21.34 Å
Total atoms total_atoms	5546
Residues n_residues	343
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	72.5 Å
Rg (real space) rg_real	21.29 Å
Rg uncertainty (real space) rg_real_error	0.42 Å
I(0) (real space) i0_real	2.5010e+07
I(0) uncertainty (real space) i0_real_error	3.2600e+05
Rg (reciprocal space) rg_reciprocal	21.31 Å
I(0) (reciprocal space) i0_reciprocal	25010000.0000
Solution quality estimate total_estimate	0.8743
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	2
Primary peak position r_peak_primary	25.6 Å
Skewness Skewness skewness	0.255
Kurtosis Kurtosis kurtosis	-0.346
Angular range angular_range	— – 0.3700 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	6280000.0000
Real-space data points n_real_points	69
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.789; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.997; Smooth: 0.999

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Description description	Putrescine-binding periplasmic protein PotF
Molecular weight formula_weight	39490.6 kDa
Mutation mutation	S87Y, A182D
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	6222

AEQKTLHIYNWSDYIAPDTVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSAYFLERQLTAGVFQPLDKSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSWDLILKPENLEKLKSCGVSFLDDPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLRPNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAFFDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPGIYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGKLEHHHHHH

来源 Source

来源生物 organism	Escherichia coli K-12
NCBI Class编号 ncbi_taxonomy_id	83333
宿主生物 host_organism	Escherichia coli BL21(DE3)

Entity 2 Entity 2 non-polymer

Description description	AGMATINE
Molecular weight formula_weight	130.2 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 3 Entity 3 non-polymer

Description description	1,2-ETHANEDIOL
Molecular weight formula_weight	62.1 kDa
Source method src_method	syn
Copy count entity_count	10

Entity 4 Entity 4 non-polymer

Description description	GLYCEROL
Molecular weight formula_weight	92.1 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 5 Entity 5 non-polymer

Description description	DI(HYDROXYETHYL)ETHER
Molecular weight formula_weight	106.1 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 6 Entity 6 non-polymer

Description description	CALCIUM ION
Molecular weight formula_weight	40.1 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 7 Entity 7 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	355

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8. Citations (1)

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Unit-cell length a length_a	37.260 Å
Unit-cell length b length_b	79.070 Å
Unit-cell length c length_c	113.340 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	4

Space group space_group	P 21 21 21
Space-group number int_tables_number	19
Crystal system crystal_system	orthorhombic

Resolution resolution	1.280 Å
Low-resolution limit resolution_low	37.330 Å
R factor r_factor	0.1801
R_work r_work	0.1787
R_free r_free	0.2087

fit	/app/data/saxs_computation/as/8asz/8asz.fit
pddf	/app/data/pr_computation/as/8asz/8asz.pr.out
profile	/app/data/saxs_computation/as/8asz/8asz.dat

PotF with mutations S87Y and A182D in complex with Agmatine

1. Protein Identity and Related Structures Protein Identity & Related Structures

Putrescine-binding periplasmic protein PotF

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction