8imy

Cryo-EM structure of GPI-T (inactive mutant) with GPI and proULBP2, a proprotein substrate

Method: ELECTRON MICROSCOPY
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1. 蛋白身份与相关结构 Protein Identity & Related Structures

Glycosylphosphatidylinositol anchor attachment 1 protein,GFP-like fluorescent chromoprotein cFP484

Clavularia sp.

UniProt O43292

当前结构中的状态

Assembly 物理组成 蛋白状态 分子拷贝数 共同组分 数据核对
1 信息不足 异源复合物 蛋白 6 其他聚合物 1 GPI-anchor transamidase,GFP-like fluorescent chromoprotein cFP484 × 1 (Q92643,Q9U6Y3) Phosphatidylinositol glycan anchor biosynthesis class U protein,GFP-like fluorescent chromoprotein cFP484 × 1 (Q9H490,Q9U6Y3) GPI transamidase component PIG-T,GFP-like fluorescent chromoprotein cFP484 × 1 (Q969N2,Q9U6Y3) GPI transamidase component PIG-S,GFP-like fluorescent chromoprotein cFP484 × 1 (Q96S52,Q9U6Y3) UL16-binding protein 2 × 1 (Q9BZM5) 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 Digitonin × 1 [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate × 6 CHOLESTEROL HEMISUCCINATE × 19 MAGNESIUM ION × 1 2-azanylethyl [(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-2,4,5-tris(oxidanyl)oxan-3-yl] hydrogen phosphate × 1 2-azanylethyl [(2R,3S,4S,5S,6S)-3,4,5,6-tetrakis(oxidanyl)oxan-2-yl]methyl hydrogen phosphate × 2 CALCIUM ION × 1 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;[(2R)-1-[[(1S,2R,3R,4S,5S,6R)-2-hexadecanoyloxy-3,4,5,6-tetrakis(oxidanyl)cyclohexyl]oxy-oxidanyl-phosphoryl]oxy-3-octadecoxy-propan-2-yl] (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate ; × 1 2-amino-2-deoxy-alpha-D-glucopyranose × 1 ;[(2R)-1-hexadecanoyloxy-3-[[3-[[(2R)-3-hexadecanoyloxy-2-[(Z)-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-2-oxidanyl-propoxy]-oxidanyl-phosphoryl]oxy-propan-2-yl] (Z)-octadec-9-enoate ; × 1 与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据
UniProt名称 GPAA1_HUMAN
Isoform —
PDB实体 1
链与序列区间 作者链 G; PDB构建体 4–623; UniProt 2–621

Glycosylphosphatidylinositol anchor attachment 1 protein,GFP-like fluorescent chromoprotein cFP484

Clavularia sp.

UniProt Q9U6Y3

当前结构中的状态

Assembly 物理组成 蛋白状态 分子拷贝数 共同组分 数据核对
1 信息不足 异源复合物 蛋白 6 其他聚合物 1 UL16-binding protein 2 × 1 (Q9BZM5) 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 Digitonin × 1 [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate × 6 CHOLESTEROL HEMISUCCINATE × 19 MAGNESIUM ION × 1 2-azanylethyl [(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-2,4,5-tris(oxidanyl)oxan-3-yl] hydrogen phosphate × 1 2-azanylethyl [(2R,3S,4S,5S,6S)-3,4,5,6-tetrakis(oxidanyl)oxan-2-yl]methyl hydrogen phosphate × 2 CALCIUM ION × 1 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;[(2R)-1-[[(1S,2R,3R,4S,5S,6R)-2-hexadecanoyloxy-3,4,5,6-tetrakis(oxidanyl)cyclohexyl]oxy-oxidanyl-phosphoryl]oxy-3-octadecoxy-propan-2-yl] (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate ; × 1 2-amino-2-deoxy-alpha-D-glucopyranose × 1 ;[(2R)-1-hexadecanoyloxy-3-[[3-[[(2R)-3-hexadecanoyloxy-2-[(Z)-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-2-oxidanyl-propoxy]-oxidanyl-phosphoryl]oxy-propan-2-yl] (Z)-octadec-9-enoate ; × 1 与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据
UniProt名称 GFPL_CLASP
Isoform —
PDB实体 1, 2, 3, 4, 5
链与序列区间 作者链 G; PDB构建体 642–857; UniProt 45–260 作者链 K; PDB构建体 416–631; UniProt 45–260 作者链 U; PDB构建体 456–671; UniProt 45–260 作者链 T; PDB构建体 599–814; UniProt 45–260 作者链 S; PDB构建体 576–791; UniProt 45–260

GPI-anchor transamidase,GFP-like fluorescent chromoprotein cFP484

Clavularia sp.

UniProt Q92643

当前结构中的状态

Assembly 物理组成 蛋白状态 分子拷贝数 共同组分 数据核对
1 信息不足 异源复合物 蛋白 6 其他聚合物 1 Glycosylphosphatidylinositol anchor attachment 1 protein,GFP-like fluorescent chromoprotein cFP484 × 1 (O43292,Q9U6Y3) Phosphatidylinositol glycan anchor biosynthesis class U protein,GFP-like fluorescent chromoprotein cFP484 × 1 (Q9H490,Q9U6Y3) GPI transamidase component PIG-T,GFP-like fluorescent chromoprotein cFP484 × 1 (Q969N2,Q9U6Y3) GPI transamidase component PIG-S,GFP-like fluorescent chromoprotein cFP484 × 1 (Q96S52,Q9U6Y3) UL16-binding protein 2 × 1 (Q9BZM5) 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 Digitonin × 1 [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate × 6 CHOLESTEROL HEMISUCCINATE × 19 MAGNESIUM ION × 1 2-azanylethyl [(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-2,4,5-tris(oxidanyl)oxan-3-yl] hydrogen phosphate × 1 2-azanylethyl [(2R,3S,4S,5S,6S)-3,4,5,6-tetrakis(oxidanyl)oxan-2-yl]methyl hydrogen phosphate × 2 CALCIUM ION × 1 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;[(2R)-1-[[(1S,2R,3R,4S,5S,6R)-2-hexadecanoyloxy-3,4,5,6-tetrakis(oxidanyl)cyclohexyl]oxy-oxidanyl-phosphoryl]oxy-3-octadecoxy-propan-2-yl] (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate ; × 1 2-amino-2-deoxy-alpha-D-glucopyranose × 1 ;[(2R)-1-hexadecanoyloxy-3-[[3-[[(2R)-3-hexadecanoyloxy-2-[(Z)-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-2-oxidanyl-propoxy]-oxidanyl-phosphoryl]oxy-propan-2-yl] (Z)-octadec-9-enoate ; × 1 与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据
UniProt名称 GPI8_HUMAN
Isoform —
PDB实体 2
链与序列区间 作者链 K; PDB构建体 4–397; UniProt 2–395

Phosphatidylinositol glycan anchor biosynthesis class U protein,GFP-like fluorescent chromoprotein cFP484

Clavularia sp.

UniProt Q9H490

当前结构中的状态

Assembly 物理组成 蛋白状态 分子拷贝数 共同组分 数据核对
1 信息不足 异源复合物 蛋白 6 其他聚合物 1 Glycosylphosphatidylinositol anchor attachment 1 protein,GFP-like fluorescent chromoprotein cFP484 × 1 (O43292,Q9U6Y3) GPI-anchor transamidase,GFP-like fluorescent chromoprotein cFP484 × 1 (Q92643,Q9U6Y3) GPI transamidase component PIG-T,GFP-like fluorescent chromoprotein cFP484 × 1 (Q969N2,Q9U6Y3) GPI transamidase component PIG-S,GFP-like fluorescent chromoprotein cFP484 × 1 (Q96S52,Q9U6Y3) UL16-binding protein 2 × 1 (Q9BZM5) 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 Digitonin × 1 [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate × 6 CHOLESTEROL HEMISUCCINATE × 19 MAGNESIUM ION × 1 2-azanylethyl [(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-2,4,5-tris(oxidanyl)oxan-3-yl] hydrogen phosphate × 1 2-azanylethyl [(2R,3S,4S,5S,6S)-3,4,5,6-tetrakis(oxidanyl)oxan-2-yl]methyl hydrogen phosphate × 2 CALCIUM ION × 1 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;[(2R)-1-[[(1S,2R,3R,4S,5S,6R)-2-hexadecanoyloxy-3,4,5,6-tetrakis(oxidanyl)cyclohexyl]oxy-oxidanyl-phosphoryl]oxy-3-octadecoxy-propan-2-yl] (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate ; × 1 2-amino-2-deoxy-alpha-D-glucopyranose × 1 ;[(2R)-1-hexadecanoyloxy-3-[[3-[[(2R)-3-hexadecanoyloxy-2-[(Z)-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-2-oxidanyl-propoxy]-oxidanyl-phosphoryl]oxy-propan-2-yl] (Z)-octadec-9-enoate ; × 1 与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据
UniProt名称 PIGU_HUMAN
Isoform —
PDB实体 3
链与序列区间 作者链 U; PDB构建体 4–437; UniProt 2–435

GPI transamidase component PIG-T,GFP-like fluorescent chromoprotein cFP484

Clavularia sp.

UniProt Q969N2

当前结构中的状态

Assembly 物理组成 蛋白状态 分子拷贝数 共同组分 数据核对
1 信息不足 异源复合物 蛋白 6 其他聚合物 1 Glycosylphosphatidylinositol anchor attachment 1 protein,GFP-like fluorescent chromoprotein cFP484 × 1 (O43292,Q9U6Y3) GPI-anchor transamidase,GFP-like fluorescent chromoprotein cFP484 × 1 (Q92643,Q9U6Y3) Phosphatidylinositol glycan anchor biosynthesis class U protein,GFP-like fluorescent chromoprotein cFP484 × 1 (Q9H490,Q9U6Y3) GPI transamidase component PIG-S,GFP-like fluorescent chromoprotein cFP484 × 1 (Q96S52,Q9U6Y3) UL16-binding protein 2 × 1 (Q9BZM5) 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 Digitonin × 1 [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate × 6 CHOLESTEROL HEMISUCCINATE × 19 MAGNESIUM ION × 1 2-azanylethyl [(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-2,4,5-tris(oxidanyl)oxan-3-yl] hydrogen phosphate × 1 2-azanylethyl [(2R,3S,4S,5S,6S)-3,4,5,6-tetrakis(oxidanyl)oxan-2-yl]methyl hydrogen phosphate × 2 CALCIUM ION × 1 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;[(2R)-1-[[(1S,2R,3R,4S,5S,6R)-2-hexadecanoyloxy-3,4,5,6-tetrakis(oxidanyl)cyclohexyl]oxy-oxidanyl-phosphoryl]oxy-3-octadecoxy-propan-2-yl] (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate ; × 1 2-amino-2-deoxy-alpha-D-glucopyranose × 1 ;[(2R)-1-hexadecanoyloxy-3-[[3-[[(2R)-3-hexadecanoyloxy-2-[(Z)-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-2-oxidanyl-propoxy]-oxidanyl-phosphoryl]oxy-propan-2-yl] (Z)-octadec-9-enoate ; × 1 与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据
UniProt名称 PIGT_HUMAN
Isoform —
PDB实体 4
链与序列区间 作者链 T; PDB构建体 4–580; UniProt 2–578

GPI transamidase component PIG-S,GFP-like fluorescent chromoprotein cFP484

Clavularia sp.

UniProt Q96S52

当前结构中的状态

Assembly 物理组成 蛋白状态 分子拷贝数 共同组分 数据核对
1 信息不足 异源复合物 蛋白 6 其他聚合物 1 Glycosylphosphatidylinositol anchor attachment 1 protein,GFP-like fluorescent chromoprotein cFP484 × 1 (O43292,Q9U6Y3) GPI-anchor transamidase,GFP-like fluorescent chromoprotein cFP484 × 1 (Q92643,Q9U6Y3) Phosphatidylinositol glycan anchor biosynthesis class U protein,GFP-like fluorescent chromoprotein cFP484 × 1 (Q9H490,Q9U6Y3) GPI transamidase component PIG-T,GFP-like fluorescent chromoprotein cFP484 × 1 (Q969N2,Q9U6Y3) UL16-binding protein 2 × 1 (Q9BZM5) 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 Digitonin × 1 [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate × 6 CHOLESTEROL HEMISUCCINATE × 19 MAGNESIUM ION × 1 2-azanylethyl [(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-2,4,5-tris(oxidanyl)oxan-3-yl] hydrogen phosphate × 1 2-azanylethyl [(2R,3S,4S,5S,6S)-3,4,5,6-tetrakis(oxidanyl)oxan-2-yl]methyl hydrogen phosphate × 2 CALCIUM ION × 1 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;[(2R)-1-[[(1S,2R,3R,4S,5S,6R)-2-hexadecanoyloxy-3,4,5,6-tetrakis(oxidanyl)cyclohexyl]oxy-oxidanyl-phosphoryl]oxy-3-octadecoxy-propan-2-yl] (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate ; × 1 2-amino-2-deoxy-alpha-D-glucopyranose × 1 ;[(2R)-1-hexadecanoyloxy-3-[[3-[[(2R)-3-hexadecanoyloxy-2-[(Z)-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-2-oxidanyl-propoxy]-oxidanyl-phosphoryl]oxy-propan-2-yl] (Z)-octadec-9-enoate ; × 1 与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据
UniProt名称 PIGS_HUMAN
Isoform —
PDB实体 5
链与序列区间 作者链 S; PDB构建体 4–557; UniProt 2–555

UL16-binding protein 2

Homo sapiens

UniProt Q9BZM5

当前结构中的状态

Assembly 物理组成 蛋白状态 分子拷贝数 共同组分 数据核对
1 信息不足 异源复合物 蛋白 6 其他聚合物 1 Glycosylphosphatidylinositol anchor attachment 1 protein,GFP-like fluorescent chromoprotein cFP484 × 1 (O43292,Q9U6Y3) GPI-anchor transamidase,GFP-like fluorescent chromoprotein cFP484 × 1 (Q92643,Q9U6Y3) Phosphatidylinositol glycan anchor biosynthesis class U protein,GFP-like fluorescent chromoprotein cFP484 × 1 (Q9H490,Q9U6Y3) GPI transamidase component PIG-T,GFP-like fluorescent chromoprotein cFP484 × 1 (Q969N2,Q9U6Y3) GPI transamidase component PIG-S,GFP-like fluorescent chromoprotein cFP484 × 1 (Q96S52,Q9U6Y3) 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 Digitonin × 1 [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate × 6 CHOLESTEROL HEMISUCCINATE × 19 MAGNESIUM ION × 1 2-azanylethyl [(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-2,4,5-tris(oxidanyl)oxan-3-yl] hydrogen phosphate × 1 2-azanylethyl [(2R,3S,4S,5S,6S)-3,4,5,6-tetrakis(oxidanyl)oxan-2-yl]methyl hydrogen phosphate × 2 CALCIUM ION × 1 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;[(2R)-1-[[(1S,2R,3R,4S,5S,6R)-2-hexadecanoyloxy-3,4,5,6-tetrakis(oxidanyl)cyclohexyl]oxy-oxidanyl-phosphoryl]oxy-3-octadecoxy-propan-2-yl] (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate ; × 1 2-amino-2-deoxy-alpha-D-glucopyranose × 1 ;[(2R)-1-hexadecanoyloxy-3-[[3-[[(2R)-3-hexadecanoyloxy-2-[(Z)-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-2-oxidanyl-propoxy]-oxidanyl-phosphoryl]oxy-propan-2-yl] (Z)-octadec-9-enoate ; × 1 与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据
UniProt名称 ULBP2_HUMAN
Isoform —
PDB实体 6
链与序列区间 作者链 D; PDB构建体 1–258; UniProt 1–246

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。 链映射与序列区间收在“数据证据”中;数据库内部编号、导入时间和 assembly 操作表达式仅用于维护,因此不在读者页面展示。

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id8imy
沉积日期 deposition_date2023-03-07
结构标题 titleCryo-EM structure of GPI-T (inactive mutant) with GPI and proULBP2, a proprotein substrate
关键词 keywordscryo-EM, glycosylphosphatidylinositol, GPI, GPI anchored protein, GPI-AP, membrane protein complex, proprotein, MEMBRANE PROTEIN; MEMBRANE PROTEIN
实验方法 methodELECTRON MICROSCOPY
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3. Official assembly/model 理论 SAXS Official SAXS Profiles

页面只读取最新 assembly 计算结果。每一条曲线都对应明确的 PDB、official biological assembly 和 coordinate model。

8imy__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

下载该曲线 .dat

8imy__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 q (1/Angstrom) I(q)
100 个绘图点;横轴和纵轴均采用对数尺度。

8imy__assembly_1__model_1 | P(r) · Pending

新版 assembly/model P(r) 尚未计算 此处仅保留位置,不展示旧数据 r (Angstrom) P(r)
P(r) 将按相同 assembly/model 分别计算和展示。
Rg(Guinier)43.18 Å
Rg(电子密度)43.09 Å
总 Rg43.46 Å
原子数19986
残基数2393
排除体积366940 ų
最大 q0.500 Å⁻¹
Assembly Model 结构单元 聚集说明 状态 CRYSOL 操作
1 1 8imy__assembly_1__model_1 hexameric (6) 成功 4.1.3-1-20251215 (887e7ef) 查看 下载
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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合物信息 Entities & Polymers (19)

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7. 引用文献 (1)