SAXSdb — 8p4g

▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

Multicopper oxidase

Pyrobaculum aerophilum str. IM2

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 2	COPPER (II) ION × 8 CHLORIDE ION × 1 water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	Q8ZWA8_PYRAE
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–440； UniProt 38–477 Author chain B； PDBConstruct 1–440； UniProt 38–477

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id	8p4g
Deposition date deposition_date	2023-05-21
Structure title title	Crystal structure of a multicopper oxidase 3F3 variant from Pyrobaculum aerophilum
Keywords keywords	hyperthermophiles, metallo-oxidases, laccases, enzyme engineering, biocatalysis, OXIDOREDUCTASE; OXIDOREDUCTASE
Experimental Method method	X-RAY DIFFRACTION

▼

3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8p4g__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8p4g__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

8p4g__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	30.39 Å
Rg (electron density)	30.03 Å
Total Rg	30.64 Å
Atom count	6786
Residues	867
Excluded volume	121370 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`8p4g__assembly_1__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download

▶

4. Crystallography and Experiment 4. Crystallography & Experiment

▶

5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Description description	Multicopper oxidase
Molecular weight formula_weight	48797.9 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B
Atom count atom_count	6949

TSAPVPPLIKEATYIEATASGYMAEGVLNPTIILRRGQRVDMTLKNKLTEPTIVHWHGFDVNWHNDAHPSFAITPGESYNYSFDVVNRAGTYLYHPHPHGLTAKQFYMGQLGLVIVEDSGSDLGFKYGVNDLPLVISDRRFIGGAPVYNPTPMEMIAGFLGNAVLVNGIKDAVFKLSGGSYRLRLVNGSNARLYMLSIVKKNGDVVPMRLIAVDQGFLARPIEVRALFLAPAERAEVVVELGEGVYLLKNTPFDHMHLEMGHGMQEALPEGSEYTIATFLVEGKGEAVPVEALPDPPPEPPKPTRTRRFALSLSGMQWTINGMSWNASNPLFEHVSVEGVELWEIVNDKASMTHPVHLHGFPMWIIERKDSPRQVAELAVDNRGRLPTDLGLKDTVLIWPGETVKIVVNFDAKKRGQLFPFHCHNLEHEDGGMMINIAVK

Source Source

Source organism organism	Pyrobaculum aerophilum str. IM2
NCBI taxonomy ID ncbi_taxonomy_id	178306
Host organism host_organism	Escherichia coli

Entity 2 Entity 2 non-polymer

Description description	COPPER (II) ION
Molecular weight formula_weight	63.5 kDa
Source method src_method	syn
Copy count entity_count	8

Entity 3 Entity 3 non-polymer

Description description	CHLORIDE ION
Molecular weight formula_weight	35.5 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 4 Entity 4 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	142

▶

Unit-cell length a length_a	128.395 Å
Unit-cell length b length_b	128.395 Å
Unit-cell length c length_c	124.965 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	16

Space group space_group	P 41 21 2
Space-group number int_tables_number	92
Crystal system crystal_system	—

Resolution resolution	2.590 Å
Low-resolution limit resolution_low	73.450 Å
R factor r_factor	0.2138
R_work r_work	0.2131
R_free r_free	0.2597

Crystal structure of a multicopper oxidase 3F3 variant from Pyrobaculum aerophilum

1. Protein Identity and Related Structures Protein Identity & Related Structures

Multicopper oxidase

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

8p4g__assembly_1__model_1

8p4g__assembly_1__model_1 | I(q)

8p4g__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 water

7. Citations (1)

Crystal structure of a multicopper oxidase 3F3 variant from Pyrobaculum aerophilum

1. Protein Identity and Related Structures Protein Identity & Related Structures

Multicopper oxidase

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

8p4g__assembly_1__model_1

8p4g__assembly_1__model_1 | I(q)

8p4g__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 water

7. Citations (1)