Large ribosomal subunit protein uL2m
OrganismNot specified
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein–RNA Heteromer Protein × 50 RNA 2 PDB declaration: 52-meric（52） Consistent with all polymer counts | Chain LB； UniProt 1–305 | Not recorded | 16s rRNA × 1 Val tRNA × 1 Large ribosomal subunit protein uL3m × 1 （P09001） Large ribosomal subunit protein uL4m × 1 （Q9BYD3） Large ribosomal subunit protein bL9m × 1 （Q9BYD2） Large ribosomal subunit protein uL10m × 1 （Q7Z7H8） Large ribosomal subunit protein uL11m × 1 （Q9Y3B7） Large ribosomal subunit protein uL13m × 1 （Q9BYD1） Large ribosomal subunit protein uL14m × 1 （Q6P1L8） Large ribosomal subunit protein uL15m × 1 （Q9P015） Large ribosomal subunit protein uL16m × 1 （Q9NX20） Large ribosomal subunit protein bL17m × 1 （Q9NRX2） Mitochondrial ribosomal protein L18, isoform CRA_b × 1 （A8K9D2） Large ribosomal subunit protein bL19m × 1 （P49406） Large ribosomal subunit protein bL20m × 1 （Q9BYC9） Large ribosomal subunit protein bL21m × 1 （Q7Z2W9） 39S ribosomal protein L22, mitochondrial × 1 （E7ESL0） Large ribosomal subunit protein uL23m × 1 （Q16540） Large ribosomal subunit protein uL24m × 1 （Q96A35） Large ribosomal subunit protein bL27m × 1 （Q9P0M9） Large ribosomal subunit protein bL28m × 1 （Q13084） Large ribosomal subunit protein uL29m × 1 （Q9HD33） Large ribosomal subunit protein uL30m × 1 （Q8TCC3） Large ribosomal subunit protein bL32m × 1 （Q9BYC8） Large ribosomal subunit protein bL33m × 1 （O75394） Large ribosomal subunit protein bL34m × 1 （Q9BQ48） Large ribosomal subunit protein bL35m × 1 （Q9NZE8） Large ribosomal subunit protein bL36m × 1 （Q9P0J6） Large ribosomal subunit protein mL37 × 1 （Q9BZE1） Large ribosomal subunit protein mL38 × 1 （Q96DV4） Large ribosomal subunit protein mL39 × 1 （Q9NYK5） Large ribosomal subunit protein mL40 × 1 （Q9NQ50） Large ribosomal subunit protein mL41 × 1 （Q8IXM3） Large ribosomal subunit protein mL42 × 1 （Q9Y6G3） Large ribosomal subunit protein mL43 × 1 （Q8N983） Large ribosomal subunit protein mL44 × 1 （Q9H9J2） Large ribosomal subunit protein mL45 × 1 （Q9BRJ2） Large ribosomal subunit protein mL46 × 1 （Q9H2W6） Large ribosomal subunit protein mL48 × 1 （Q96GC5） Large ribosomal subunit protein mL49 × 1 （Q13405） Large ribosomal subunit protein mL50 × 1 （Q8N5N7） Large ribosomal subunit protein mL51 × 1 （Q4U2R6） 39S ribosomal protein L52, mitochondrial × 1 （A8K7J6） Large ribosomal subunit protein mL53 × 1 （Q96EL3） Large ribosomal subunit protein mL54 × 1 （Q6P161） Large ribosomal subunit protein mL55 × 1 （Q7Z7F7） Large ribosomal subunit protein mL63 × 1 （Q9BQC6） Large ribosomal subunit protein mL62 × 1 （Q14197） Large ribosomal subunit protein mL64 × 1 （Q8TAE8） Large ribosomal subunit protein mL66 × 1 （Q9NVS2） Large ribosomal subunit protein mL65 × 1 （Q9NP92） MG MAGNESIUM ION × 95 T1C TIGECYCLINE × 1 ZN ZINC ION × 3 | ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE | Resolution 3.10 Å |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 8XT3 | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 3IY9 Leishmania Tarentolae Mitochondrial Large Ribosomal Subunit Model Deposited 2009-04-20 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric |
Chain B
126–261（136 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
50 mM Tris HCL, pH 7.5, 100mM KCL, 10mM MgCl2, 3mM DTT, 0.1mM EDTA, 0.05% dodecyl maltoside；pH 7.5；50 mM Tris HCL, pH 7.5, 100mM KCL, 10mM MgCl2, 3mM DTT, 0.1mM EDTA, 0.05% dodecyl maltoside
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 14.10 Å |
| 3J7Y Structure of the large ribosomal subunit from human mitochondria Deposited 2014-08-26 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric |
Chain D
1–305（305 aa）
|
Not recorded | AMP ADENOSINE MONOPHOSPHATE × 1 MG MAGNESIUM ION × 66 ZN ZINC ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
20 mM HEPES-KOH, pH 7.45, 100 mM potassium chloride, 20 mM magnesium acetate, 2 mM DTT；pH 7.45；20 mM HEPES-KOH, pH 7.45, 100 mM potassium chloride, 20 mM magnesium acetate, 2 mM DTT
cryo-EM vitrification conditions
90 K；Cryogen ETHANE；Blot 2.5 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
|
Resolution 3.40 Å |
| 3J9M Structure of the human mitochondrial ribosome (class 1) Deposited 2015-02-08 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 85-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 127 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
20 mM HEPES-KOH, pH 7.45, 100 mM KCl, 20 mM MgOAc, 2 mM DTT；pH 7.45；20 mM HEPES-KOH, pH 7.45, 100 mM KCl, 20 mM MgOAc, 2 mM DTT
cryo-EM vitrification conditions
Blot 2.5 seconds before plunging；90 K；Cryogen ETHANE；Blot 2.5 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
|
Resolution 3.50 Å |
| 5OOL Structure of a native assembly intermediate of the human mitochondrial ribosome with unfolded interfacial rRNA Deposited 2017-08-08 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 56-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 92 ZN ZINC ION × 3 PNS 4'-PHOSPHOPANTETHEINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.45；2 mM Synercid (Santa Cruz Biotechnology, Inc) was added to reduce preferential orientation.
cryo-EM vitrification conditions
Cryogen ETHANE；Sample incubated for 30 s prior to freezing.
|
Resolution 3.06 Å |
| 5OOM Structure of a native assembly intermediate of the human mitochondrial ribosome with unfolded interfacial rRNA Deposited 2017-08-08 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 55-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 49 ZN ZINC ION × 2 PNS 4'-PHOSPHOPANTETHEINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.45；2 mM Synercid (Santa Cruz Biotechnology, Inc) was added to reduce preferential orientation.
cryo-EM vitrification conditions
Cryogen ETHANE；Sample incubated for 30 s prior to freezing.
|
Resolution 3.03 Å |
| 6I9R Large subunit of the human mitochondrial ribosome in complex with Virginiamycin M and Quinupristin Deposited 2018-11-25 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 53-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 MG MAGNESIUM ION × 134 H8T Virginiamycin M × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6NU2 Structural insights into unique features of the human mitochondrial ribosome recycling Deposited 2019-01-30 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 86-meric |
Chain D
61–296（236 aa）
|
Not recorded | MG MAGNESIUM ION × 127 ZN ZINC ION × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6NU3 Structural insights into unique features of the human mitochondrial ribosome recycling Deposited 2019-01-30 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 85-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 127 ZN ZINC ION × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.40 Å |
| 6VLZ Structure of the human mitochondrial ribosome-EF-G1 complex (ClassI) Deposited 2020-01-27 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 85 PDB declaration: 90-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 130 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.97 Å |
| 6VMI Structure of the human mitochondrial ribosome-EF-G1 complex (ClassIII) Deposited 2020-01-28 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 85 PDB declaration: 91-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 130 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.96 Å |
| 6ZM5 Human mitochondrial ribosome in complex with OXA1L, mRNA, A/A tRNA, P/P tRNA and nascent polypeptide Deposited 2020-07-01 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 189 K POTASSIUM ION × 44 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ALA ALANINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.89 Å |
| 6ZM6 Human mitochondrial ribosome in complex with mRNA, A/A tRNA and P/P tRNA Deposited 2020-07-01 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 86 PDB declaration: 92-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 189 K POTASSIUM ION × 44 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.59 Å |
| 6ZS9 Human mitochondrial ribosome in complex with ribosome recycling factor Deposited 2020-07-15 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 91-meric |
Chain XD
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 195 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 6ZSA Human mitochondrial ribosome bound to mRNA, A-site tRNA and P-site tRNA Deposited 2020-07-15 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 86 PDB declaration: 92-meric |
Chain XD
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 195 H8Q ~{N}-[(3~{S},6~{R},12~{R},15~{S},16~{S},19~{S},22~{S},25~{S})-25-[[(3~{S})-1-azabicyclo[2.2.2]octan-3-yl]sulfanylmethyl]-3-[[4-(dimethylamino)phenyl]methyl]-12-ethyl-4,16-dimethyl-2,5,11,14,18,21,24-heptakis(oxidanylidene)-19-phenyl-17-oxa-1,4,10,13,20-pentazatricyclo[20.4.0.0^{6,10}]hexacosan-15-yl]-3-oxidanyl-pyridine-2-carboxamide × 1 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 6ZSB Human mitochondrial ribosome in complex with mRNA and P-site tRNA Deposited 2020-07-15 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 92-meric |
Chain XD
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 195 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 6ZSC Human mitochondrial ribosome in complex with E-site tRNA Deposited 2020-07-15 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 91-meric |
Chain XD
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 195 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6ZSD Human mitochondrial ribosome in complex with mRNA, P-site tRNA and E-site tRNA Deposited 2020-07-15 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric |
Chain XD
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 195 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6ZSE Human mitochondrial ribosome in complex with mRNA, A/P-tRNA and P/E-tRNA Deposited 2020-07-15 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric |
Chain XD
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 195 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.00 Å |
| 6ZSG Human mitochondrial ribosome in complex with mRNA, A-site tRNA, P-site tRNA and E-site tRNA Deposited 2020-07-15 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 94-meric |
Chain XD
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 195 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 7A5F Structure of the stalled human mitoribosome with P- and E-site mt-tRNAs Deposited 2020-08-21 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric |
Chain D3
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 128 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 SO4 SULFATE ION × 1 MLI MALONATE ION × 3 CL CHLORIDE ION × 2 NA SODIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.40 Å |
| 7A5G Structure of the elongating human mitoribosome bound to mtEF-Tu.GMPPCP and A/T mt-tRNA Deposited 2020-08-21 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 86 PDB declaration: 94-meric |
Chain D3
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 128 SPD SPERMIDINE × 1 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 NA SODIUM ION × 4 SO4 SULFATE ION × 1 MLI MALONATE ION × 3 CL CHLORIDE ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.33 Å |
| 7A5H Structure of the split human mitoribosomal large subunit with rescue factors mtRF-R and MTRES1 Deposited 2020-08-21 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 59-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 92 ZN ZINC ION × 2 PNS 4'-PHOSPHOPANTETHEINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 7A5I Structure of the human mitoribosome with A- P-and E-site mt-tRNAs Deposited 2020-08-21 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 90-meric |
Chain D3
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 127 ZN ZINC ION × 7 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 7A5J Structure of the split human mitoribosomal large subunit with P-and E-site mt-tRNAs Deposited 2020-08-21 | Different oligomeric state Different ligand/ion | Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 63-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 92 ZN ZINC ION × 3 CL CHLORIDE ION × 2 NA SODIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 7A5K Structure of the human mitoribosome in the post translocation state bound to mtEF-G1 Deposited 2020-08-21 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 86 PDB declaration: 93-meric |
Chain D3
1–305（305 aa）
|
Not recorded | GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 128 SPD SPERMIDINE × 1 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 7L08 Cryo-EM structure of the human 55S mitoribosome-RRFmt complex. Deposited 2020-12-11 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 87-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 127 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.49 Å |
| 7L20 Cryo-EM structure of the human 39S mitoribosomal subunit in complex with RRFmt and EF-G2mt. Deposited 2020-12-15 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 57-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 102 ZN ZINC ION × 3 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.15 Å |
| 7O9K Human mitochondrial ribosome large subunit assembly intermediate with MTERF4-NSUN4, MRM2, MTG1, the MALSU module, GTPBP5 and mtEF-Tu Deposited 2021-04-16 | Different oligomeric state Different ligand/ion | Assembly 1 Protein–RNA Heteromer；Protein × 67 PDB declaration: 69-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 93 SAM S-ADENOSYLMETHIONINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 PNS 4'-PHOSPHOPANTETHEINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 7O9M Human mitochondrial ribosome large subunit assembly intermediate with MTERF4-NSUN4, MRM2, MTG1 and the MALSU module Deposited 2021-04-16 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 64 PDB declaration: 66-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 104 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 2 SAM S-ADENOSYLMETHIONINE × 1 PNS 4'-PHOSPHOPANTETHEINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 7ODR State A of the human mitoribosomal large subunit assembly intermediate Deposited 2021-04-30 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 57-meric |
Chain D
1–305（305 aa）
|
Not recorded | PM8 S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE × 1 SAM S-ADENOSYLMETHIONINE × 1 ZN ZINC ION × 2 MG MAGNESIUM ION × 97 K POTASSIUM ION × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；4 uL of the sample was blotted for 2-6 sec before plunging
|
Resolution 2.90 Å |
| 7ODS State B of the human mitoribosomal large subunit assembly intermediate Deposited 2021-04-30 | Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 58-meric |
Chain D
1–305（305 aa）
|
Not recorded | PM8 S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE × 1 SAM S-ADENOSYLMETHIONINE × 1 ZN ZINC ION × 2 MG MAGNESIUM ION × 88 K POTASSIUM ION × 8 N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；4 uL of the sample was blotted for 2-6 sec before plunging
|
Resolution 3.10 Å |
| 7ODT State C of the human mitoribosomal large subunit assembly intermediate Deposited 2021-04-30 | Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 58-meric |
Chain D
1–305（305 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 95 PM8 S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE × 1 SAM S-ADENOSYLMETHIONINE × 1 ZN ZINC ION × 2 K POTASSIUM ION × 10 FES FE2/S2 (INORGANIC) CLUSTER × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；4 uL of the sample was blotted for 2-6 sec before plunging
|
Resolution 3.10 Å |
| 7OF0 Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with MTERF4-NSUN4 (dataset1). Deposited 2021-05-04 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 56-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 MG MAGNESIUM ION × 61 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.20 Å |
| 7OF2 Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with GTPBP6. Deposited 2021-05-04 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 56-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 MG MAGNESIUM ION × 85 GTP GUANOSINE-5'-TRIPHOSPHATE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 7OF3 Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with MTERF4-NSUN4 (dataset2). Deposited 2021-05-04 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 56-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 MG MAGNESIUM ION × 58 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 7OF4 Structure of mature human mitochondrial ribosome large subunit in complex with GTPBP6 (PTC conformation 1). Deposited 2021-05-04 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 53-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 MG MAGNESIUM ION × 89 GTP GUANOSINE-5'-TRIPHOSPHATE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 7OF5 Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with MTERF4-NSUN4 and GTPBP5 (dataset2). Deposited 2021-05-04 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 57-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 MG MAGNESIUM ION × 70 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 7OF6 Structure of mature human mitochondrial ribosome large subunit in complex with GTPBP6 (PTC conformation 2). Deposited 2021-05-04 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 53-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 MG MAGNESIUM ION × 88 GTP GUANOSINE-5'-TRIPHOSPHATE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 7OF7 Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with MTERF4-NSUN4 and GTPBP5 (dataset1). Deposited 2021-05-04 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 57-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 MG MAGNESIUM ION × 74 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 7OG4 Human mitochondrial ribosome in complex with P/E-tRNA Deposited 2021-05-06 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 86 PDB declaration: 90-meric |
Chain XD
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 195 H8Q ~{N}-[(3~{S},6~{R},12~{R},15~{S},16~{S},19~{S},22~{S},25~{S})-25-[[(3~{S})-1-azabicyclo[2.2.2]octan-3-yl]sulfanylmethyl]-3-[[4-(dimethylamino)phenyl]methyl]-12-ethyl-4,16-dimethyl-2,5,11,14,18,21,24-heptakis(oxidanylidene)-19-phenyl-17-oxa-1,4,10,13,20-pentazatricyclo[20.4.0.0^{6,10}]hexacosan-15-yl]-3-oxidanyl-pyridine-2-carboxamide × 1 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 7OI6 Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 1 Deposited 2021-05-11 | Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 52-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 48 ZN ZINC ION × 1 PNS 4'-PHOSPHOPANTETHEINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.70 Å |
| 7OI7 Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 2 Deposited 2021-05-11 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 54-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 49 PNS 4'-PHOSPHOPANTETHEINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 7OI8 Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 3A Deposited 2021-05-11 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 49 ZN ZINC ION × 2 PNS 4'-PHOSPHOPANTETHEINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 7OI9 Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 3B Deposited 2021-05-11 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 54-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 49 ZN ZINC ION × 2 PNS 4'-PHOSPHOPANTETHEINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 7OIA Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 3C Deposited 2021-05-11 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 49 ZN ZINC ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 7OIB Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 3D Deposited 2021-05-11 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 49 ZN ZINC ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 7OIC Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 4 Deposited 2021-05-11 | Different oligomeric state Different ligand/ion | Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 58-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 76 ZN ZINC ION × 3 PNS 4'-PHOSPHOPANTETHEINE × 1 SAM S-ADENOSYLMETHIONINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 7OID Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 5A Deposited 2021-05-11 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 56-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 2 PNS 4'-PHOSPHOPANTETHEINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 7OIE Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 5B Deposited 2021-05-11 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 55-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 2 PNS 4'-PHOSPHOPANTETHEINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 7PD3 Structure of the human mitoribosomal large subunit in complex with NSUN4.MTERF4.GTPBP7 and MALSU1.L0R8F8.mt-ACP Deposited 2021-08-04 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 60-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 91 PNS 4'-PHOSPHOPANTETHEINE × 1 SAM S-ADENOSYLMETHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 7PO4 Assembly intermediate of human mitochondrial ribosome large subunit (largely unfolded rRNA with MALSU1, L0R8F8 and ACP) Deposited 2021-09-08 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 60-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 35 K POTASSIUM ION × 8 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.56 Å |
| 7QH6 Cryo-EM structure of the human mtLSU assembly intermediate upon MRM2 depletion - class 1 Deposited 2021-12-10 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 46-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.08 Å |
| 7QH7 Cryo-EM structure of the human mtLSU assembly intermediate upon MRM2 depletion - class 4 Deposited 2021-12-10 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 49-meric |
Chain D
61–275（215 aa）
|
Not recorded | MG MAGNESIUM ION × 50 ZN ZINC ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.89 Å |
| 7QI4 Human mitochondrial ribosome at 2.2 A resolution (bound to partly built tRNAs and mRNA) Deposited 2021-12-14 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 94-meric |
Chain D
1–305（305 aa）
|
Not recorded | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 SPD SPERMIDINE × 4 MG MAGNESIUM ION × 206 K POTASSIUM ION × 54 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PUT 1,4-DIAMINOBUTANE × 1 VAL VALINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.21 Å |
| 7QI5 Human mitochondrial ribosome in complex with mRNA, A/A-, P/P- and E/E-tRNAs at 2.63 A resolution Deposited 2021-12-14 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 94-meric |
Chain D
1–305（305 aa）
|
Not recorded | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 SPD SPERMIDINE × 4 MG MAGNESIUM ION × 208 K POTASSIUM ION × 54 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PUT 1,4-DIAMINOBUTANE × 1 VAL VALINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.63 Å |
| 7QI6 Human mitochondrial ribosome in complex with mRNA, A/P- and P/E-tRNAs at 2.98 A resolution Deposited 2021-12-14 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric |
Chain D
1–305（305 aa）
|
Not recorded | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 54 MG MAGNESIUM ION × 202 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PUT 1,4-DIAMINOBUTANE × 1 VAL VALINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.98 Å |
| 8ANY Human mitochondrial ribosome in complex with LRPPRC, SLIRP, A-site, P-site, E-site tRNAs and mRNA Deposited 2022-08-06 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 96-meric |
Chain D
1–305（305 aa）
|
Not recorded | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 SPD SPERMIDINE × 4 MG MAGNESIUM ION × 206 K POTASSIUM ION × 49 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PUT 1,4-DIAMINOBUTANE × 1 VAL VALINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.85 Å |
| 8K2A Cryo-EM structure of the human 55S mitoribosome with Tigecycline Deposited 2023-07-12 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 83-meric |
Chain LB
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 145 T1C TIGECYCLINE × 4 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8K2B Cryo-EM structure of the human 39S mitoribosome with Tigecycline Deposited 2023-07-12 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 55-meric |
Chain LB
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 95 T1C TIGECYCLINE × 1 ZN ZINC ION × 3 PNS 4'-PHOSPHOPANTETHEINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8OIR 55S human mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 | Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric |
Chain BL
1–305（305 aa）
|
Not recorded | K POTASSIUM ION × 51 MG MAGNESIUM ION × 354 VAL VALINE × 1 ZN ZINC ION × 3 FS2 FE-S-O HYBRID CLUSTER × 3 MET METHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.10 Å |
| 8OIT 39S human mitochondrial large ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 62-meric |
Chain BL
1–305（305 aa）
|
Not recorded | K POTASSIUM ION × 34 MG MAGNESIUM ION × 228 VAL VALINE × 1 ZN ZINC ION × 2 FS2 FE-S-O HYBRID CLUSTER × 1 MET METHIONINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.90 Å |
| 8PK0 human mitoribosomal large subunit assembly intermediate 1 with GTPBP10-GTPBP7 Deposited 2023-06-23 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 58-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 1 PM8 S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE × 1 SAM S-ADENOSYLMETHIONINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 MG MAGNESIUM ION × 96 K POTASSIUM ION × 10 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.03 Å |
| 8QSJ Human mitoribosomal large subunit assembly intermediate 2 with GTPBP7 Deposited 2023-10-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 58-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 38 FES FE2/S2 (INORGANIC) CLUSTER × 1 PM8 S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE × 1 SAM S-ADENOSYLMETHIONINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8QU1 mt-LSU assembly intermediate in GTPBP8 knock-out cells, state 1 Deposited 2023-10-13 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 30-meric |
Chain D
1–305（305 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.74 Å |
| 8QU5 mt-LSU assembly intermediate in GTPBP8 knock-out cells, state 2 Deposited 2023-10-13 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 55-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 48 PNS 4'-PHOSPHOPANTETHEINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.42 Å |
| 8RRI Human mitochondrial ribosome in complex with antibiotic tigecycline Deposited 2024-01-22 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric |
Chain D
1–305（305 aa）
|
Not recorded | VAL VALINE × 1 MG MAGNESIUM ION × 94 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 T1C TIGECYCLINE × 3 K POTASSIUM ION × 16 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 8XT0 Cryo-EM structure of the human 55S mitoribosome with 5um Tigecycline Deposited 2024-01-10 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 83-meric |
Chain LB
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 145 T1C TIGECYCLINE × 3 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8XT1 Cryo-EM structure of the human 39S mitoribosome with 5uM Tigecycline Deposited 2024-01-10 | Parsed fields agree | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 52-meric |
Chain LB
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 95 T1C TIGECYCLINE × 1 ZN ZINC ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8XT2 Cryo-EM structure of the human 55S mitoribosome with 10uM Tigecycline Deposited 2024-01-10 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 83-meric |
Chain LB
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 145 T1C TIGECYCLINE × 3 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9CN3 Human 39S mitoribosome in complex with antibiotic Linezolid Deposited 2024-07-15 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 56-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 MG MAGNESIUM ION × 102 ZLD N-{[(5S)-3-(3-fluoro-4-morpholin-4-ylphenyl)-2-oxo-1,3-oxazolidin-5-yl]methyl}acetamide × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.62 Å |
| 9OLF Membrane-associated human mitoribosome in complex with TACO1 Deposited 2025-05-12 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 93 PDB declaration: 100-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 SPD SPERMIDINE × 7 MG MAGNESIUM ION × 205 PUT 1,4-DIAMINOBUTANE × 1 K POTASSIUM ION × 54 SPM SPERMINE × 2 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 VAL VALINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.46 Å |
| 9PG8 In situ structure of the human mitoribosome in the P-E state Deposited 2025-07-07 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 86 PDB declaration: 93-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 8 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 205 VAL VALINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.06 Å |
| 9PGF In situ structure of the human mitoribosome in the P state Deposited 2025-07-07 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 86 PDB declaration: 91-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 8 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 205 VAL VALINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.93 Å |
| 9PGI In situ structure of the human mitoribosome in the A-P-E state with TACO1 Deposited 2025-07-07 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 94-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.02 Å |
| 9PGL In situ structure of the human mitoribosome in the A-P state with TACO1 Deposited 2025-07-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 86 PDB declaration: 92-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 8 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9PGM In situ structure of the human mitoribosome in the P-E state from TACO1-knockout cells Deposited 2025-07-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.18 Å |
| 9PR4 In Situ Structure of the Human Mitochondrial Large Subunit 39S in Complex with TACO1 Deposited 2025-07-23 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 61 PDB declaration: 63-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 2 K POTASSIUM ION × 36 MG MAGNESIUM ION × 141 FES FE2/S2 (INORGANIC) CLUSTER × 1 SPD SPERMIDINE × 2 PUT 1,4-DIAMINOBUTANE × 1 VAL VALINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.77 Å |
| 9PRA In Situ Structure of the Human Mitoribosome Large Subunit 39S in Complex with EF-Tu Deposited 2025-07-23 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 64 PDB declaration: 66-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 2 K POTASSIUM ION × 36 MG MAGNESIUM ION × 141 FES FE2/S2 (INORGANIC) CLUSTER × 1 SPD SPERMIDINE × 2 PUT 1,4-DIAMINOBUTANE × 1 VAL VALINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.83 Å |
| 9PRD In situ structure of the human mitoribosome in the P state from TACO1-knockout cells Deposited 2025-07-23 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.31 Å |
| 9PRQ In situ structure of human mitoribosome in the A/T-P state from TACO1-knockout cells Deposited 2025-07-24 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.34 Å |
| 9PRX In situ structure of the human mitoribosome in the A-P state from TACO1-knockout cells Deposited 2025-07-24 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 90-meric |
Chain D
1–305（305 aa）
|
Not recorded | VAL VALINE × 1 ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.23 Å |
| 9PS0 In situ structure of the human mitoribosome in the A/P-P/E state from TACO1-knockout cells Deposited 2025-07-24 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 K POTASSIUM ION × 52 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.29 Å |
| 9PS7 In situ structure of the human mitoribosome in the A/T-P-E state from TACO1-knockout cells Deposited 2025-07-25 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 86 PDB declaration: 93-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.08 Å |
| 9PSI In situ structure of the human mitoribosome in the A-P-E state from TACO1-knockout cells Deposited 2025-07-25 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 92-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 7 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.12 Å |
| 9PSM In situ structure of the human mitoribosome in the A/P-P/E state Deposited 2025-07-25 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric |
Chain D
1–305（305 aa）
|
Not recorded | ZN ZINC ION × 3 K POTASSIUM ION × 54 SPD SPERMIDINE × 8 PUT 1,4-DIAMINOBUTANE × 1 MG MAGNESIUM ION × 204 FES FE2/S2 (INORGANIC) CLUSTER × 3 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 VAL VALINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.98 Å |
| 9UWH human mitoribosome trapped by retapamulin Deposited 2025-05-12 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric |
Chain D
1–305（305 aa）
|
Not recorded | MG MAGNESIUM ION × 208 K POTASSIUM ION × 53 G34 Retapamulin × 1 VAL VALINE × 1 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
86 other PDB entries and 86 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | RM02_HUMAN |
| Isoform | — |
| PDB entities | 3 |
| Chains and sequence ranges | Author chain LB； PDBConstruct 1–305； UniProt 1–305 |
