Gamma-aminobutyric acid receptor subunit beta-3
Homo sapiens
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Other combination Homooligomer Protein × 5 其他Polymer 10 PDB declaration: pentameric（5） Consistent with protein copy count | Chain A； UniProt 26–332 Chain A； UniProt 447–473 Chain B； UniProt 26–332 Chain B； UniProt 447–473 Chain C； UniProt 26–332 Chain C； UniProt 447–473 Chain D； UniProt 26–332 Chain D； UniProt 447–473 Chain E； UniProt 26–332 Chain E； UniProt 447–473 | Not recorded | ;alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 5 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 | ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO cryo-EM vitrification conditions：Cryogen ETHANE | Resolution 3.10 Å |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 9FEV | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 4COF Crystal structure of a human gamma-aminobutyric acid receptor, the GABA(A)R-beta3 homopentamer Deposited 2014-01-28 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–312（287 aa）
Fragment：RESIDUES 26-312,447-473
Chain A
447–473（27 aa）
Fragment：RESIDUES 26-312,447-473
Chain B
26–312（287 aa）
Fragment：RESIDUES 26-312,447-473
Chain B
447–473（27 aa）
Fragment：RESIDUES 26-312,447-473
Chain C
26–312（287 aa）
Fragment：RESIDUES 26-312,447-473
Chain C
447–473（27 aa）
Fragment：RESIDUES 26-312,447-473
Chain D
26–312（287 aa）
Fragment：RESIDUES 26-312,447-473
Chain D
447–473（27 aa）
Fragment：RESIDUES 26-312,447-473
Chain E
26–312（287 aa）
Fragment：RESIDUES 26-312,447-473
Chain E
447–473（27 aa）
Fragment：RESIDUES 26-312,447-473
|
Not recorded | BEN BENZAMIDINE × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 CL CHLORIDE ION × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；11.5% PEG4000 100 MM SODIUM CHLORIDE, 100 MM LITHIUM SULPHATE, 100 MM N-2-ACETAMIDO-IMINODIACETIC ACID, PH 6.5, 2% (W/V) BENZAMIDINE
|
Resolution 2.97 Å R-free 0.226 |
| 5O8F Structure of a chimaeric beta3-alpha5 GABAA receptor in complex with nanobody Nb25 and pregnanolone Deposited 2017-06-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
26–246（221 aa）
Chain B
26–246（221 aa）
Chain C
26–246（221 aa）
Chain D
26–246（221 aa）
Chain E
26–246（221 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 P9N Pregnanolone × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.6；277 K；14% PEG 6000, 0.1M N-(2-Acetamido)iminodiacetic acid (ADA), 7.6 mM 4-Cyclohexyl-1-Butyl-beta-D-Maltoside (CYMAL-4)
|
Resolution 3.20 Å R-free 0.242 |
| 5OJM Structure of a chimaeric beta3-alpha5 GABAA receptor in complex with nanobody Nb25 Deposited 2017-07-21 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
26–242（217 aa）
Chain B
26–242（217 aa）
Chain C
26–242（217 aa）
Chain D
26–242（217 aa）
Chain E
26–242（217 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.8；277.15 K；14% poly-ethylene glycol (PEG) 5000, 0.08M magnesium acetate, 0.1M sodium citrate, 1mM ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA)
|
Resolution 3.30 Å R-free 0.250 |
| 6A96 Cryo-EM structure of the human alpha5beta3 GABAA receptor in complex with GABA and Nb25 Deposited 2018-07-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–332（332 aa）
Chain B
447–473（27 aa）
Chain C
1–332（332 aa）
Chain C
447–473（27 aa）
Chain D
1–332（332 aa）
Chain D
447–473（27 aa）
Chain E
1–332（332 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.51 Å |
| 6HUG CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with picrotoxin and megabody Mb38. Deposited 2018-10-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 RI5 (1aR,2aR,3S,6R,6aS,8aS,8bR,9R)-2a-hydroxy-8b-methyl-9-(prop-1-en-2-yl)hexahydro-3,6-methano-1,5,7-trioxacyclopenta[ij]c yclopropa[a]azulene-4,8(3H)-dione × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6HUJ CryoEM structure of human full-length heteromeric alpha1beta3gamma2L GABA(A)R in complex with picrotoxin, GABA and megabody Mb38. Deposited 2018-10-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 RI5 (1aR,2aR,3S,6R,6aS,8aS,8bR,9R)-2a-hydroxy-8b-methyl-9-(prop-1-en-2-yl)hexahydro-3,6-methano-1,5,7-trioxacyclopenta[ij]c yclopropa[a]azulene-4,8(3H)-dione × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.04 Å |
| 6HUK CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with bicuculline and megabody Mb38. Deposited 2018-10-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | H0Z bicuculline methochloride × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.69 Å |
| 6HUO CryoEM structure of human full-length heteromeric alpha1beta3gamma2L GABA(A)R in complex with alprazolam (Xanax), GABA and megabody Mb38. Deposited 2018-10-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 08H 8-chloro-1-methyl-6-phenyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.26 Å |
| 6HUP CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with diazepam (Valium), GABA and megabody Mb38. Deposited 2018-10-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 DZP 7-CHLORO-1-METHYL-5-PHENYL-1,3-DIHYDRO-2H-1,4-BENZODIAZEPIN-2-ONE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.58 Å |
| 6I53 Cryo-EM structure of the human synaptic alpha1-beta3-gamma2 GABAA receptor in complex with Megabody38 in a lipid nanodisc Deposited 2018-11-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 3 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6QFA CryoEM structure of a beta3K279T GABA(A)R homomer in complex with histamine and megabody Mb25 Deposited 2019-01-09 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
26–332（307 aa）
Chain A
447–473（27 aa）
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain D
26–332（307 aa）
Chain D
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Mutation：K279T Mutation：K279T Mutation：K279T Mutation：K279T Mutation：K279T Mutation：K279T Mutation：K279T Mutation：K279T Mutation：K279T Mutation：K279T | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 HSM HISTAMINE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.49 Å |
| 7A5V CryoEM structure of a human gamma-aminobutyric acid receptor, the GABA(A)R-beta3 homopentamer, in complex with histamine and megabody Mb25 in lipid nanodisc Deposited 2020-08-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: pentamer |
Chain A
26–332（307 aa）
Chain A
447–473（27 aa）
|
Not recorded | R16 HEXADECANE × 5 D10 DECANE × 20 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 OCT N-OCTANE × 5 HSM HISTAMINE × 5 CL CHLORIDE ION × 10 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.70 Å |
| 7PBD a1b3 GABA-A receptor + GABA Deposited 2021-08-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
33–332（300 aa）
Chain B
447–473（27 aa）
Chain C
33–332（300 aa）
Chain C
447–473（27 aa）
Chain E
33–332（300 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 HSM HISTAMINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.04 Å |
| 7PBZ a1b3 GABA-A receptor + GABA + Zn2+ Deposited 2021-08-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 UND UNDECANE × 2 OCT N-OCTANE × 2 HEX HEXANE × 4 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ZN ZINC ION × 1 HSM HISTAMINE × 1 D10 DECANE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.79 Å |
| 7PC0 GABA-A receptor bound by a-Cobratoxin Deposited 2021-08-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
33–332（300 aa）
Chain B
447–473（27 aa）
Chain C
33–332（300 aa）
Chain C
447–473（27 aa）
Chain E
33–332（300 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 HSM HISTAMINE × 1 ZN ZINC ION × 1 HEX HEXANE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 7QN5 Cryo-EM structure of human full-length extrasynaptic alpha4beta3delta GABA(A)R in complex with nanobody Nb25 Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain D
1–473（473 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 PX6 1,2-DIPALMITOYL-SN-GLYCERO-3-PHOSPHATE × 1 D10 DECANE × 2 OCT N-OCTANE × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 CL CHLORIDE ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 7QN6 Cryo-EM structure of human full-length beta3delta GABA(A)R in complex with nanobody Nb25 Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–473（473 aa）
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain D
1–473（473 aa）
|
Not recorded | EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 7QN7 Cryo-EM structure of human full-length extrasynaptic alpha4beta3delta GABA(A)R in complex with GABA, histamine and nanobody Nb25 Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain D
1–473（473 aa）
|
Not recorded | PX6 1,2-DIPALMITOYL-SN-GLYCERO-3-PHOSPHATE × 1 D10 DECANE × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 ABU GAMMA-AMINO-BUTANOIC ACID × 1 R16 HEXADECANE × 2 HSM HISTAMINE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 7QN8 Cryo-EM structure of human full-length beta3delta GABA(A)R in complex with histamine and nanobody Nb25 Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–473（473 aa）
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain D
1–473（473 aa）
|
Not recorded | HSM HISTAMINE × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 7QN9 Cryo-EM structure of human full-length extrasynaptic alpha4beta3delta GABA(A)R in complex with GABA, histamine and nanobody Nb25 in a pre-open/closed state Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain D
1–473（473 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 HSM HISTAMINE × 3 R16 HEXADECANE × 1 D10 DECANE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 7QNA Cryo-EM structure of human full-length alpha4beta3gamma2 GABA(A)R in complex with GABA and nanobody Nb25 Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain D
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 7QNB Cryo-EM structure of human full-length beta3gamma2 GABA(A)R in complex with GABA and nanobody Nb25 Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain D
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 7QNC Cryo-EM structure of human full-length extrasynaptic alpha4beta3delta GABA(A)R in complex with THIP (gaboxadol), histamine and nanobody Nb25 Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain D
1–473（473 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 D10 DECANE × 3 EI7 4,5,6,7-tetrahydro-[1,2]oxazolo[5,4-c]pyridin-3-one × 2 HSM HISTAMINE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 7QND Cryo-EM structure of human full-length extrasynaptic beta3delta GABA(A)R in complex with THIP (gaboxadol), histamine and nanobody Nb25 Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–473（473 aa）
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain D
1–473（473 aa）
|
Not recorded | HSM HISTAMINE × 3 EI7 4,5,6,7-tetrahydro-[1,2]oxazolo[5,4-c]pyridin-3-one × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 7QNE Cryo-EM structure of human full-length synaptic alpha1beta3gamma2 GABA(A)R in complex with Ro15-4513 and megabody Mb38 Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 OCT N-OCTANE × 1 D10 DECANE × 4 CL CHLORIDE ION × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 EIE ethyl 8-[(azanylidene-$l^{4}-azanylidene)amino]-5-methyl-6-oxidanylidene-4~{H}-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 8PET Cryo-EM structure of the full-length human alpha1beta3 GABA(A) receptor (babba arrangement) in complex with nanobody Nb25, expressed with a 5-fold excess of beta3 over alpha1 Deposited 2023-06-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–473（448 aa）
Chain D
26–473（448 aa）
Chain E
26–473（448 aa）
|
Not recorded | R16 HEXADECANE × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 12 ZN ZINC ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 8PVB Structure of GABAAR determined by cryoEM at 100 keV Deposited 2023-07-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
26–332（307 aa）
|
Not recorded | R16 HEXADECANE × 5 D10 DECANE × 20 HSM HISTAMINE × 5 CL CHLORIDE ION × 10 V8D Etomidate × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 9CSB Native human GABAA receptor of beta3-alpha1-beta2-alpha2-gamma2 assembly Deposited 2024-07-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
26–473（448 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 s Blot.
|
Resolution 3.34 Å |
| 9CTJ Native human GABAA receptor of beta2-alpha1-beta3-alpha2-gamma2 assembly Deposited 2024-07-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain C
26–473（448 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 s Blot.
|
Resolution 3.74 Å |
| 9CX7 Native human GABAA receptor of beta3-alpha1-gamma2-beta3-alpha2 assembly Deposited 2024-07-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
26–473（448 aa）
Chain D
26–473（448 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 s Blot.
|
Resolution 3.30 Å |
| 9CXA Native human GABAA receptor of beta2-alpha1-beta3-alpha1-gamma2 assembly Deposited 2024-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain C
1–473（473 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.04 Å |
| 9CXC Native human GABAA receptor of beta3-alpha1-gamma2-beta2-alpha2 assembly Deposited 2024-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
26–473（448 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 s Blot.
|
Resolution 3.30 Å |
| 9EQG CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with GABA and puerarin Deposited 2024-03-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 2 D10 DECANE × 11 HEX HEXANE × 10 PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 2 R16 HEXADECANE × 5 PLM PALMITIC ACID × 3 CL CHLORIDE ION × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 A1H6W Puerarin × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 100 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 9FAJ CryoEM structure of human full-length alpha1beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, GABA and Megabody38, in a desensitised state (StateD1) Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain B
32–472（441 aa）
Chain E
32–472（441 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 D10 DECANE × 12 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 5 ABU GAMMA-AMINO-BUTANOIC ACID × 2 CL CHLORIDE ION × 4 R16 HEXADECANE × 4 HEX HEXANE × 7 PLM PALMITIC ACID × 5 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 1 CLR CHOLESTEROL × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9FAK CryoEM structure of human full-length alpha1beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, GABA and Megabody38 in a desensitised state (StateD2) Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain B
32–472（441 aa）
Chain E
32–472（441 aa）
|
Not recorded | HEX HEXANE × 6 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 5 D10 DECANE × 7 PLM PALMITIC ACID × 8 CL CHLORIDE ION × 4 ABU GAMMA-AMINO-BUTANOIC ACID × 2 R16 HEXADECANE × 3 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 1 CLR CHOLESTEROL × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9FAM CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2, GABA and Megabody38 in a desensitised state (StateD3) Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
32–472（441 aa）
Chain E
32–472（441 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 CL CHLORIDE ION × 3 HEX HEXANE × 7 D10 DECANE × 1 PLM PALMITIC ACID × 1 CLR CHOLESTEROL × 1 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 9FAP CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2 and Megabody38 in a closed state (StateC1) Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
34–472（439 aa）
Chain E
34–472（439 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 2 PLM PALMITIC ACID × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CLR CHOLESTEROL × 1 D10 DECANE × 1 HEX HEXANE × 2 CL CHLORIDE ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9FAQ CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2 and Megabody38 in a closed state (StateC2) Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
34–472（439 aa）
Chain E
34–472（439 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 D10 DECANE × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PLM PALMITIC ACID × 5 CLR CHOLESTEROL × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 HEX HEXANE × 2 CL CHLORIDE ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9FAS CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with pregnenolone sulfate Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
32–472（441 aa）
Chain E
32–472（441 aa）
|
Not recorded | HEX HEXANE × 8 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 3 D10 DECANE × 17 PLM PALMITIC ACID × 3 CL CHLORIDE ION × 3 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 R16 HEXADECANE × 5 A8W Pregnenolone sulfate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 9FAT CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2, Megabody38 and pregnenolone sulfate Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
32–472（441 aa）
Chain E
32–472（441 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 3 D10 DECANE × 3 CL CHLORIDE ION × 2 R16 HEXADECANE × 1 HEX HEXANE × 3 PLM PALMITIC ACID × 1 CLR CHOLESTEROL × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 A8W Pregnenolone sulfate × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 9FAU CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2 and Megabody25, in a closed state (StateC1) Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
34–472（439 aa）
Chain B
34–472（439 aa）
Chain D
34–472（439 aa）
Chain E
34–472（439 aa）
|
Not recorded | HEX HEXANE × 6 D10 DECANE × 6 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 2 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 R16 HEXADECANE × 3 PLM PALMITIC ACID × 2 CLR CHOLESTEROL × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FAV CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2 and Megabody25, in a closed state (StateC2) Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
34–472（439 aa）
Chain B
34–472（439 aa）
Chain D
34–472（439 aa）
Chain E
34–472（439 aa）
|
Not recorded | HEX HEXANE × 5 D10 DECANE × 4 R16 HEXADECANE × 3 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CLR CHOLESTEROL × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9FAW CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, HEPES and Megabody25, in a desensitised state Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
30–472（443 aa）
Chain B
30–472（443 aa）
Chain D
30–472（443 aa）
Chain E
30–472（443 aa）
|
Not recorded | HEX HEXANE × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 3 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 CLR CHOLESTEROL × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9FAX CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with Megabody25, doubly occupied GARLH4 and Neuroligin2 TMD, in a closed state Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
34–472（439 aa）
Chain B
34–472（439 aa）
Chain D
34–472（439 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 R16 HEXADECANE × 5 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 2 CLR CHOLESTEROL × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 4 HEX HEXANE × 13 D10 DECANE × 5 PLM PALMITIC ACID × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9FEU Cryo-EM structure of the beta3 homomeric GABA(A) receptor in complex with HSM in the long-lived symmetric desensitised state Deposited 2024-05-21 | Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–332（307 aa）
Chain A
447–473（27 aa）
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain D
26–332（307 aa）
Chain D
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 HSM HISTAMINE × 5 D10 DECANE × 15 CL CHLORIDE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 9FEW Cryo-EM structure of the beta3 homomeric GABA(A) receptor in the long-lived resting state (C1) Deposited 2024-05-21 | Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–332（307 aa）
Chain A
447–473（27 aa）
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain D
26–332（307 aa）
Chain D
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 9FEX Cryo-EM structure of the beta3 homomeric GABA(A) receptor in complex with HSM in the short-lived symmetric bound-closed state (C5) Deposited 2024-05-21 | Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–332（307 aa）
Chain A
447–473（27 aa）
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain D
26–332（307 aa）
Chain D
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 HSM HISTAMINE × 5 CL CHLORIDE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9FEY Cryo-EM structure of the beta3 homomeric GABA(A) receptor in complex with HSM in the short-lived symmetric bound-closed state (C1) Deposited 2024-05-21 | Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–332（307 aa）
Chain A
447–473（27 aa）
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain D
26–332（307 aa）
Chain D
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 HSM HISTAMINE × 5 CL CHLORIDE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9FEZ Cryo-EM structure of the beta3 homomeric GABA(A) receptor in complex with HSM in the short-lived asymmetric bound-closed state Deposited 2024-05-21 | Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–332（307 aa）
Chain A
447–473（27 aa）
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain D
26–332（307 aa）
Chain D
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 HSM HISTAMINE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9FF0 Cryo-EM structure of the beta3 homomeric GABA(A) receptor in complex with HSM in the short-lived asymmetric open state Deposited 2024-05-21 | Different construct Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
29–332（304 aa）
Chain A
447–473（27 aa）
Chain B
29–332（304 aa）
Chain B
447–473（27 aa）
Chain C
29–332（304 aa）
Chain C
447–473（27 aa）
Chain D
29–332（304 aa）
Chain D
447–473（27 aa）
Chain E
29–332（304 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 HSM HISTAMINE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9FF1 Cryo-EM structure of the beta3 homomeric GABA(A) receptor in complex with HSM in the short-lived asymmetric desensitised state Deposited 2024-05-21 | Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–332（307 aa）
Chain A
447–473（27 aa）
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain D
26–332（307 aa）
Chain D
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 HSM HISTAMINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9FF2 Cryo-EM structure of the beta3 homomeric GABA(A) receptor in complex with HSM in the long-lived symmetric desensitised state (C1) Deposited 2024-05-21 | Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–332（307 aa）
Chain A
447–473（27 aa）
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain D
26–332（307 aa）
Chain D
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 HSM HISTAMINE × 5 D10 DECANE × 15 CL CHLORIDE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9FFL Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived symmetric bound-closed state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | D10 DECANE × 6 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 CL CHLORIDE ION × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 R16 HEXADECANE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9FFM Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with Mb25 in the resting state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | D10 DECANE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9FFN Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric bound-closed 1 state of branch 1 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | D10 DECANE × 3 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 CL CHLORIDE ION × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FFO Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric bound-closed 2 state of branch 1 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 D10 DECANE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9FFP Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric open state of branch 1 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 9FFQ Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric desensitised state of branch 1 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | CL CHLORIDE ION × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FFR Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric bound-closed state of branch 2 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | CLR CHOLESTEROL × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FFS Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric open state of branch 2 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9FFT Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric desensitised state of branch 2 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FFU Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | D10 DECANE × 6 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 CL CHLORIDE ION × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 R16 HEXADECANE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 9FFV Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with Nb38 in the long-lived symmetric resting state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | D10 DECANE × 5 CL CHLORIDE ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9FFW Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived symmetric bound-closed state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 D10 DECANE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9FFX Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric bound-closed state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 9FFY Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric open1 state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FFZ Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric desensitised 1 state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 1 CLR CHOLESTEROL × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9FG0 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric open 2 state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
33–332（300 aa）
Chain B
447–472（26 aa）
Chain E
33–332（300 aa）
Chain E
447–472（26 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 9FG1 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric desensitised 2 state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 2 D10 DECANE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FG2 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 2 CL CHLORIDE ION × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 D10 DECANE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9FG3 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 bound twice in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | D10 DECANE × 5 D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FG4 Cryo-EM structure of the full-length alpha1beta3 GABA(A) receptor in the long-lived symmetric resting state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9FG5 Cryo-EM structure of the full-length alpha1beta3 GABA(A) receptor in complex with GABA in the short-lived symmetric bound-closed state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9FG6 Cryo-EM structure of the full-length alpha1beta3 GABA(A) receptor in complex with GABA and HSM in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 HSM HISTAMINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9FG7 Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in complex with GABA in the short-lived symmetric bound-closed state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 D10 DECANE × 3 CL CHLORIDE ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9FG8 Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in complex with GABA in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | CL CHLORIDE ION × 1 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 5 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9FG9 Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Etomidate in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | CL CHLORIDE ION × 3 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 5 LBN 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine × 2 R16 HEXADECANE × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 V8D Etomidate × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9FGA Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs bound to two PIP2 molecules and in complex with Mb38 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9FGB Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs bound to one PIP2 molecule at chain alpha1-A and in complex with Mb38 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 9FGC Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs bound to one PIP2 molecule at chain alpha1-D and in complex with Mb38 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | D10 DECANE × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9FGD Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs without PIP2 and in complex with Mb38 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 D10 DECANE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9FGF Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in Saposin A nanodisc in the long-lived symmetric resting state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | CL CHLORIDE ION × 3 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 R16 HEXADECANE × 2 D10 DECANE × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9FGG Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in Saposin A nanodisc bound to GABA and Etomidate in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 5 CL CHLORIDE ION × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 V8D Etomidate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 R16 HEXADECANE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9FGH Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in large MSP2N2 nanodisc in complex with GABA in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 CL CHLORIDE ION × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9G2D Cryo-EM structure of the beta3 homomeric GABA(A) receptor in the long-lived resting state (C5) sprayed with PBS Deposited 2024-07-10 | Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–332（307 aa）
Chain B
26–332（307 aa）
Chain C
26–332（307 aa）
Chain D
26–332（307 aa）
Chain E
26–332（307 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 CL CHLORIDE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9G2E Cryo-EM structure of the beta3 homomeric GABA(A) receptor in the long-lived desensitised state (C5) sprayed with PBS Deposited 2024-07-10 | Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–332（307 aa）
Chain B
26–332（307 aa）
Chain C
26–332（307 aa）
Chain D
26–332（307 aa）
Chain E
26–332（307 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 D10 DECANE × 10 CL CHLORIDE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 9HAA a5b3 GABAA Receptor resting state Deposited 2024-11-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
1–332（332 aa）
Chain B
447–473（27 aa）
Chain C
1–332（332 aa）
Chain C
447–473（27 aa）
Chain E
1–332（332 aa）
Chain E
447–473（27 aa）
|
Not recorded | BUA butanoic acid × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 ZN ZINC ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；20 mM HEPES, 100 mM NaCl, 2 mM Fluorinated Fos-choline 8, 0.005% LMNG, 0.0005% CHS
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.14 Å |
| 9HNQ a5b3 GABAA Receptor bound to GABA and Mb25 in desensitized state in detergent micelles Deposited 2024-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–332（332 aa）
Chain B
447–473（27 aa）
Chain C
1–332（332 aa）
Chain C
447–473（27 aa）
Chain E
1–332（332 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；20 mM HEPES, 100 mM NaCl, 0.005% LMNG, 0.0005% CHS
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.81 Å |
| 9HNR a5b3 GABAA Receptor in 1 a5 to 4 b3 stoichiometry in desensitized state Deposited 2024-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–332（332 aa）
Chain B
447–473（27 aa）
Chain C
1–332（332 aa）
Chain C
447–473（27 aa）
Chain D
1–332（332 aa）
Chain D
447–473（27 aa）
Chain E
1–332（332 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.17 Å |
| 9HNS a5b3 GABAAR bound to GABA and Mb25 in a desensitized state in saposin nanodiscs after long GABA treatment Deposited 2024-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–332（332 aa）
Chain B
447–473（27 aa）
Chain C
1–332（332 aa）
Chain C
447–473（27 aa）
Chain E
1–332（332 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9HNT a5b3 GABAAR bound to Etomidate, GABA, and Mb25 in a desensitized state in saposin nanodiscs Deposited 2024-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–332（332 aa）
Chain B
447–473（27 aa）
Chain C
1–332（332 aa）
Chain C
447–473（27 aa）
Chain E
1–332（332 aa）
Chain E
447–473（27 aa）
|
Not recorded | V8D Etomidate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.32 Å |
| 9HUM a5b3 GABAAR bound to GABA and Mb25 in a desensitized state in saposin nanodiscs after short GABA treatment Deposited 2024-12-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–332（332 aa）
Chain C
1–332（332 aa）
Chain E
1–332（332 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.35 Å |
| 9RL5 a5b3 GABAAR bound to Topiramate, GABA, and Mb25 in a desensitized state in saposin nanodiscs Deposited 2025-06-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–332（332 aa）
Chain B
447–473（27 aa）
Chain C
1–332（332 aa）
Chain C
447–473（27 aa）
Chain E
1–332（332 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 TOR [(3aS,5aR,8aR,8bS)-2,2,7,7-tetramethyltetrahydro-3aH-bis[1,3]dioxolo[4,5-b:4',5'-d]pyran-3a-yl]methyl sulfamate × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9RPB a5b3 GABAAR bound to GABA, and Mb25 in a desensitized state in saposin nanodiscs, topiramate-free Deposited 2025-06-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–332（332 aa）
Chain B
447–473（27 aa）
Chain C
1–332（332 aa）
Chain C
447–473（27 aa）
Chain E
1–332（332 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
94 other PDB entries and 94 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | GBRB3_HUMAN |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 55–361； UniProt 26–332 Author chain A； PDBConstruct 369–395； UniProt 447–473 Author chain B； PDBConstruct 55–361； UniProt 26–332 Author chain B； PDBConstruct 369–395； UniProt 447–473 Author chain C； PDBConstruct 55–361； UniProt 26–332 Author chain C； PDBConstruct 369–395； UniProt 447–473 Author chain D； PDBConstruct 55–361； UniProt 26–332 Author chain D； PDBConstruct 369–395； UniProt 447–473 Author chain E； PDBConstruct 55–361； UniProt 26–332 Author chain E； PDBConstruct 369–395； UniProt 447–473 |
