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9ffj

Artificial metalloenzyme with a nickel-based 1,10-phenanthroline cofactor and streptavidin N49M-S112V mutant

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Streptavidin

Streptomyces avidinii

UniProt P22629

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein homooligomer Homooligomer Protein 4 1,2-ETHANEDIOL × 8 ;N-methyl-N-[(4,4,6,6-tetrahydroxy-4,6-dioxido-1,3,3a,5,6a-tetrahydrothien[3,4-d]imidazol-4-ium-2-yl)methyl]-5-(2,4,4-trihydroxy-2-keto-3,3a,5,6-tetrahydro-1H-thien[3,4-d]imidazol-4-ium-6-yl ; × 4 DI(HYDROXYETHYL)ETHER × 8 SULFATE ION × 8 AMMONIUM ION × 4 water × 4 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
3rdm 3rdo 3rdq 3rds 3rdu 3re5 3re6 5n8t 7dy0 7dy0 7efc 7efc

14 assemblies; showing the first 12

Homodimer
1mm9 1moy 1swg 1swg 3rdx 5cse 6s4q 6s50 6uc3 6ud1 6ud6 8xg4

17 assemblies; showing the first 12

Homotetramer
1df8 1i9h 1kff 1kl4 1lcv 1lcw 1lcz 1mep 1mk5 1mm9 1mm9 1moy

262 assemblies; showing the first 12

Other homooligomers
1df8 1lcv 1lcz 1sre 1srg 1sri 2izc 2izd 2ize 2rth 2rti 2rtn

16 assemblies; showing the first 12

Heteromeric or nucleic-acid complex
1hqq 1hxl 1hxz 1hy2 1kl3 1kl5 1pts 1pts 1rst 1rsu 1sld 1sle

60 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name SAV_STRAV
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 15–159； UniProt 39–183

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id9ffj
Deposition date deposition_date2024-05-23
Structure title titleArtificial metalloenzyme with a nickel-based 1,10-phenanthroline cofactor and streptavidin N49M-S112V mutant
Keywords keywordsArtificial metalloenzyme, nickel, METAL BINDING PROTEIN; METAL BINDING PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

9ffj__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

9ffj__assembly_1__model_1 | I(q)

10-2 10-1 105 106 107 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

9ffj__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）22.60 Å
Rg (electron density)21.58 Å
Total Rg22.57 Å
Atom count7500
Residues484
Excluded volume67855 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 9ffj__assembly_1__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (7)

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7. Citations (1)