9fr1

Half-closed CODH/ACS in the as-isolated state

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

CO-methylating acetyl-CoA synthase

Carboxydothermus hydrogenoformans

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 4	CO-dehydrogenase × 2 Fe(3)-Ni(1)-S(4) cluster × 2 IRON/SULFUR CLUSTER × 5 NICKEL (II) ION × 2 SODIUM ION × 1 water × 3	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	P83789_CARHY
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain C； PDBConstruct 1–728； UniProt 5–732 Author chain D； PDBConstruct 1–728； UniProt 5–732

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	9fr1
Deposition date deposition_date	2024-06-18
Structure title title	Half-closed CODH/ACS in the as-isolated state
Keywords keywords	CODH, ACS, CO2 fixation, reductive acetyl-CoA pathway, Wood-Ljungdahl pathway, OXIDOREDUCTASE; OXIDOREDUCTASE
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

9fr1__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

9fr1__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

9fr1__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	44.71 Å
Rg (electron density)	45.06 Å
Total Rg	44.90 Å
Atom count	18555
Residues	2379
Excluded volume	332900 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`9fr1__assembly_1__model_1`	tetrameric （4）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Description description	CO-dehydrogenase
Molecular weight formula_weight	73172.1 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B
Atom count atom_count	18799

PRFRDLEHTSKPSKADRVWEPKNRKRTIDPAALEMLEKAEKDGVKTAFDRFVEMQPQCQFGYKGLCCRFCLQGPCRLPNDDPSKKGICGASAWTIAARSVGTLILTGAAAHNEHARHIAHALKELAEGKAPDYKITDPDKLRRIAQRLGLDTQGKDDMTLAKEVAELALEDFARLPGFGENLWIKTTLNKERLEKYDECNIMPSGIFGDISDLLAQAHIGNDDDPVNITFSALRVALTDYAGMHIATDFSDVLFGTPKPIVTEANLGVLDANKVNIAVHGHNPLLSEKVVDAAKELEEEAKAAGAEGINIVGMCCTGNEVLMRRGVHLATSFASSELAIVTGAMDAVVVDVQCIMPGLKQVTECYHTRLITTSNIAKMPGTYHVPFHIENALESAKEIVRLGIEAFKQRVGKPVHIPEVKHKVVAGFSFEALMEIFAHVNQENPIRVLNDAILSGQLKGVVLFAGCNNLKRPQDESHITILKEMLKNDVFVVTTGCSAQAFAKHGFLRPEALELAGEGLKSFIKMLEEKAGLQGQLPPAFFMGSCVDNTRASDILVAMAKDLGVDTPKVPFVASAPEAMSGKAVSIGTWFVTLGVPVHVGTMPPLEGSELFYSITTQIASDVYGGYFMFEVDPVVAARKILNALEYRTWKLGVHKQTAEKFETALCQNY

Source Source

Source organism organism	Carboxydothermus hydrogenoformans
NCBI taxonomy ID ncbi_taxonomy_id	129958
Host organism host_organism	Escherichia coli

Entity 2 Entity 2 polymer

Description description	CO-methylating acetyl-CoA synthase
Molecular weight formula_weight	82111.2 kDa
EC 编号 ec_number	2.3.1.169
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C,D

INFDQIFEGAIEPGKEPKRLFKEVYEGAITATSYAEILLSRAIEKYGPDHPVGYPDTAYFLPVIRAFSGEEVRTLKDMVPILNRMRAQIKSELTFENARLAGEATWYAAEIIEALRYLKHTPENPIVVPPWTGFIGDPVVRQYGIKMVDWTIPGEAIIIGRAKDSKAAKKIVDDLMGKGLMLFLCDEIIEQLLEENVKLGVDYIAYPLGNFTQVVHAANYALRAGLMFGGIAPGLRDAHRDYQRRRVLAFVLYLGEHDMVKTAAAMGAIFTGFPVITDQPLPEDKQIKDWFISEPDYDKIVQTALEVRGIKITSIDIDLPINFGPAFEGESIRKGDMHVEFGGGKTPSFELVRMVGPDEIEDGKVEVIGPDIDSVEPGGRLPIGIVVDIYGRKMQEDFEPVLERRIHYFTNYGEGFWHTAQRDLTWVRISKEAFAKGARLKHLGQLLYAKFKQEFPSIVDRVQVTIYTDEQKVLELREIARKKYAERDARLRELSDEAVDTYYSCLLCQSFAPTHVCIVSPERVGLCGAISWLDAKAAYEINPNGPNQPIPKEGLIDPVKGQWESFNEYIYKNSQRTIERMNLYTIMEYPMTSCGCFEAIMAYLPELNGFMIVNREHSGMTPIGMTFSTLAGMVGGGTQTPGFMGIGKSYIGSRKFVKADGGLARVVWMPKDLKEQLRSIIEERAEEEGLGRDFIDKIADETVGTTVDEVLPFLEEKGHPALSMEPLLRS

Source Source

Source organism organism	Carboxydothermus hydrogenoformans
NCBI taxonomy ID ncbi_taxonomy_id	129958
Host organism host_organism	Escherichia coli

Entity 3 Entity 3 non-polymer

Description description	Fe(3)-Ni(1)-S(4) cluster
Molecular weight formula_weight	410.3 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 4 Entity 4 non-polymer

Description description	IRON/SULFUR CLUSTER
Molecular weight formula_weight	351.6 kDa
Source method src_method	syn
Copy count entity_count	5

Entity 5 Entity 5 non-polymer

Description description	NICKEL (II) ION
Molecular weight formula_weight	58.7 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 6 Entity 6 non-polymer

Description description	SODIUM ION
Molecular weight formula_weight	23.0 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 7 Entity 7 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	238

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	triclinic

Resolution resolution	2.20 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	139853

Half-closed CODH/ACS in the as-isolated state

1. Protein Identity and Related Structures Protein Identity & Related Structures

CO-methylating acetyl-CoA synthase

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

9fr1__assembly_1__model_1

9fr1__assembly_1__model_1 | I(q)

9fr1__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 non-polymer

Entity 7 Entity 7 water

7. Citations (1)

Half-closed CODH/ACS in the as-isolated state

1. Protein Identity and Related Structures Protein Identity & Related Structures

CO-methylating acetyl-CoA synthase

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

9fr1__assembly_1__model_1

9fr1__assembly_1__model_1 | I(q)

9fr1__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 non-polymer

Entity 7 Entity 7 water

7. Citations (1)