Exendin-4
Heloderma suspectum
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain A； UniProt 62–86 | Not recorded | No other associated polymer | SOLUTION NMR NMR measurement conditions：pH 7.1；288 K；Ionic strength (raw mmCIF value) n.d.；Pressure 1 NMR sample composition：0.85 mM non-labeled polypeptide, 1 % non-labeled sodium azide, 1 % non-labeled DSS, 90% H2O/10% D2O | 90% H2O/10% D2O | Resolution not provided |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 9G2N | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1JRJ Solution structure of exendin-4 in 30-vol% trifluoroethanol Deposited 2001-08-13 | Different construct Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–39（39 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 5.9；280 K；Ionic strength (raw mmCIF value) 15mM potassium phosphate；Pressure ambient
NMR sample composition
Natural abundance | 2mg/ml in pH 5.9 15mM phosphate buffer with 30 vol-% trifluoroethanol added
|
Resolution not provided |
| 3C59 Crystal structure of the ligand-bound glucagon-like peptide-1 receptor extracellular domain Deposited 2008-01-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
56–86（31 aa）
Fragment：UNP residues 56-86
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 10M decyl 4-O-alpha-D-glucopyranosyl-1-thio-beta-D-glucopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.1M Tris-HCl pH 8.5, 0.1M MgCl2, 0.4M MgTartrate, 9mM n-Decyl-beta-D-thiomaltoside, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.30 Å R-free 0.241 |
| 3C5T Crystal structure of the ligand-bound glucagon-like peptide-1 receptor extracellular domain Deposited 2008-02-01 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
56–86（31 aa）
Fragment：UNP residues 56-86
|
Not recorded | 10M decyl 4-O-alpha-D-glucopyranosyl-1-thio-beta-D-glucopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.1M Tris-HCl pH 8.5, 0.1M MgCl2, 0.4M MgTartrate, 9mM n-Decyl-beta-D-thiomaltoside, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.10 Å R-free 0.238 |
| 5OTT Extracellular domain of GLP-1 receptor in complex with exendin-4 variant Gly2Hcs/Thr5Hcs Deposited 2017-08-22 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
48–86（39 aa）
|
Mutation：G2HCS, T5HCS Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M Imidazole pH 6.5, 1.0 M Sodium acetate trihydrate
|
Resolution 1.92 Å R-free 0.209 |
| 6GDZ exendin-4 based dual GLP-1/glucagon receptor agonist Deposited 2018-04-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–86（39 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | EVT (2~{S})-2-[[(4~{S})-4-(hexadecanoylamino)-5-oxidanyl-5-oxidanylidene-pentanoyl]amino]pentanedioic acid × 1 |
SOLUTION NMR
NMR measurement conditions
pH 5；310 K；Ionic strength (raw mmCIF value) 35mM sodium phosphate；Pressure 1
NMR sample composition
5 mg/mL Peptide 5, trifluoroethanol/water | trifluoroethanol/water
|
Resolution not provided |
| 6GE2 exendin-4 based dual GLP-1/glucagon receptor agonist Deposited 2018-04-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–86（39 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | EVT (2~{S})-2-[[(4~{S})-4-(hexadecanoylamino)-5-oxidanyl-5-oxidanylidene-pentanoyl]amino]pentanedioic acid × 1 |
SOLUTION NMR
NMR measurement conditions
pH 5；310 K；Ionic strength (raw mmCIF value) 35mM sodium phosphate；Pressure 1
NMR sample composition
5 mg/mL Peptide 12, trifluoroethanol/water | trifluoroethanol/water
|
Resolution not provided |
| 7LLL Exendin-4-bound Glucagon-Like Peptide-1 (GLP-1) Receptor in complex with Gs protein Deposited 2021-02-04 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain P
48–86（39 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 7MLL Solution structure of Exenatide (exendin-4) in 30-vol% trifluoroethanol using CS-Rosetta Deposited 2021-04-28 | Different construct Different mutation/modification Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–86（39 aa）
Fragment：UNP Residue 48-86
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 5.35；315 K；Ionic strength (raw mmCIF value) 20；Pressure 1
NMR sample composition
13 mM 'NA-' Exenatide (exendin-4), 20 mM 'NA-' sodium phosphate, 30 % v/v [U-99% 2H] TFE, 10 % v/v [U-99% 2H] D2O, 0.01 mg/mL 'NA-' DSS, 60 % H2O/30%Trifluoroethanol/10 % D2O | 60 % H2O/30%Trifluoroethanol/10 % D2O
|
Resolution not provided |
| 9G0M Trp-cage fortified Tc5b-Exenatide chimera (Ex4-Tc5bER) at 288K Deposited 2024-07-08 | Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
62–86（25 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；288 K；Ionic strength (raw mmCIF value) n.d.；Pressure 1
NMR sample composition
0.8 mM polypeptide, 1 % sodium-azide, 1 % DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9G0N Trp-cage fortified Tc5b-Exenatide chimera (Ex4-Tc5bER) at 299K Deposited 2024-07-08 | Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
62–86（25 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；299 K；Ionic strength (raw mmCIF value) n.d.；Pressure 1
NMR sample composition
0.8 mM non-labelled polypeptide, 1 % non-labelled sodium-azide, 1 % non-labelled DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9G20 Trp-cage fortified Tc5b-Exenatide chimera (Ex4-Tc5bER) at 310K Deposited 2024-07-10 | Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
62–86（25 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；310 K；Ionic strength (raw mmCIF value) n.d.；Pressure 1
NMR sample composition
0.8 mM non-labeled polypeptide, 1 % non-labeled sodium azide, 1 % non-labeled DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9G21 Trp-cage fortified Tc5b-Exenatide chimera (Ex4-Tc5bER) at 321K Deposited 2024-07-10 | Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
62–86（25 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；321 K；Ionic strength (raw mmCIF value) n.d.；Pressure 1
NMR sample composition
0.8 mM non-labeled polypeptide, 1 % non-labeled sodium azide, 1 % non-labeled DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9G22 Trp-cage fortified Tc5b-Exenatide chimera (Ex4-Tc5bDR) at 277K Deposited 2024-07-10 | Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
62–86（25 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7.1；277 K；Ionic strength (raw mmCIF value) n.d.；Pressure 1
NMR sample composition
0.85 mM non-labeled polypeptide, 1 % non-labeled sodium azide, 1 % non-labeled DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9G2O Trp-cage fortified Tc5b-Exenatide chimera (Ex-4-Tc5bDR) at 299K Deposited 2024-07-11 | Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
62–86（25 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7.1；299 K；Ionic strength (raw mmCIF value) n.d.；Pressure 1
NMR sample composition
0.85 mM non-labeled polypeptide, 1 % non-labeled sodium azide, 1 % non-labeled DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9G31 Trp-cage fortified Tc5b-Exenatide chimera (Ex-4-Tc5bDR) at 310K Deposited 2024-07-11 | Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
62–86（25 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7.1；310 K；Ionic strength (raw mmCIF value) n.d.；Pressure 1
NMR sample composition
0.85 mM non-labeled polypeptide, 1 % non-labeled sodium azide, 1 % non-labeled DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9G32 Trp-cage fortified Tc5b-Exenatide chimera (Ex-4-Tc5bDR) at 321K Deposited 2024-07-11 | Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
62–86（25 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7.1；321 K；Ionic strength (raw mmCIF value) n.d.；Pressure 1
NMR sample composition
0.85 mM non-labeld polypeptide, 1 mM non-labeld sodium azide, 1 % non-labeld DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9G5P Trp-cage fortified Tc5b-Exenatide chimera (Ex4-Tc5bER) at 277K Deposited 2024-07-17 | Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
62–86（25 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；277 K；Ionic strength (raw mmCIF value) n.d.；Pressure 1
NMR sample composition
0.8 mM non-labeled polypeptide, 1 % non-labeled sodium azide, 1 % non-labeled DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
17 other PDB entries and 17 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | EXE4_HELSU |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–25； UniProt 62–86 |
