SAXSdb — 9lex

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Ligand-gated cation channel ZACN,Genome polyprotein

Human enterovirus 71

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Insufficient information Homooligomer Protein × 5 其他Polymer 10 PDB declaration: pentameric（5） Consistent with protein copy count	Chain A； UniProt 4–241 Chain B； UniProt 4–241 Chain C； UniProt 4–241 Chain D； UniProt 4–241 Chain E； UniProt 4–241	Mutation：A152T	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 10 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 2.85 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 9LEX	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8SOJ Cryo-EM structure of human CST bound to POT1(ESDL)/TPP1 in the absence of telomeric ssDNA Deposited 2023-04-28	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric	Chain B 2–241（240 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
8SOK Cryo-EM structure of human CST bound to POT1(ESDL)/TPP1 in the presence of telomeric ssDNA Deposited 2023-04-28	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: hexameric	Chain E 2–241（240 aa）	Not recorded	ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.10 Å
9LET Cryo-EM structure of human ZAC in zinc Binding State Deposited 2025-01-07	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 4–241（238 aa） Chain B 4–241（238 aa） Chain C 4–241（238 aa） Chain D 4–241（238 aa） Chain E 4–241（238 aa）	Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T	ZN ZINC ION × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.62 Å
9LEU Cryo-EM structure of human ZAC with A152 mutant in zinc binding state Deposited 2025-01-07	Different ligand/ion Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 4–241（238 aa） Chain B 4–241（238 aa） Chain C 4–241（238 aa） Chain D 4–241（238 aa） Chain E 4–241（238 aa）	Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T	ZN ZINC ION × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.63 Å
9LEV Cryo-EM structure of human ZAC in nanodisc in apo state Deposited 2025-01-07	Different ligand/ion Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 4–241（238 aa） Chain B 4–241（238 aa） Chain C 4–241（238 aa） Chain D 4–241（238 aa） Chain E 4–241（238 aa）	Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.35 Å
9LEY Cryo-EM structure of human ZAC in complex with d-tubocurarine Deposited 2025-01-07	Different ligand/ion Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 4–241（238 aa） Chain B 4–241（238 aa） Chain C 4–241（238 aa） Chain D 4–241（238 aa） Chain E 4–241（238 aa）	Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T	TC9 D-TUBOCURARINE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.97 Å
9LEZ Cryo-EM structure of human ZAC in complex with N-(4-(tert-butyl)thiazol-2-yl)-3-fluorobenzamide (TTFB) Deposited 2025-01-07	Different ligand/ion Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 4–241（238 aa） Chain B 4–241（238 aa） Chain C 4–241（238 aa） Chain D 4–241（238 aa） Chain E 4–241（238 aa）	Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T	A1EKJ ~{N}-(4-~{tert}-butyl-1,3-thiazol-2-yl)-3-fluoranyl-benzamide × 5 ZN ZINC ION × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.54 Å
9LRC Cryo-EM structure of the histamine H4 receptor-Gi protein complex (Receptor focused) Deposited 2025-01-30	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric	Chain R 2–241（240 aa）	Not recorded	HSM HISTAMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.84 Å
9LRE Cryo-EM structure of the histamine H4 receptor-Gi protein complex (Overall) Deposited 2025-01-30	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric	Chain R 2–241（240 aa）	Not recorded	HSM HISTAMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.84 Å
9R6H Crystal Structure of the M18BP1 SANTA domain as a EGFP-SANTA-nanobody fusion Deposited 2025-05-12	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric	Chain A 4–228（225 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	PG4 TETRAETHYLENE GLYCOL × 6	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；0.1M MES pH 6.0, 50% PEG400, 5% w/v PEG3000	Resolution 2.42 Å R-free 0.262
9R6H Crystal Structure of the M18BP1 SANTA domain as a EGFP-SANTA-nanobody fusion Deposited 2025-05-12	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric	Chain B 4–228（225 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	PG4 TETRAETHYLENE GLYCOL × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；0.1M MES pH 6.0, 50% PEG400, 5% w/v PEG3000	Resolution 2.42 Å R-free 0.262

10 other PDB entries and 11 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	B6F2F5_HE71
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 468–705； UniProt 4–241 Author chain B； PDBConstruct 468–705； UniProt 4–241 Author chain C； PDBConstruct 468–705； UniProt 4–241 Author chain D； PDBConstruct 468–705； UniProt 4–241 Author chain E； PDBConstruct 468–705； UniProt 4–241

Ligand-gated cation channel ZACN,Genome polyprotein

Human enterovirus 71

UniProt Q401N2

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Insufficient information Homooligomer Protein × 5 其他Polymer 10 PDB declaration: pentameric（5） Consistent with protein copy count	Chain A； UniProt 1–412 Chain B； UniProt 1–412 Chain C； UniProt 1–412 Chain D； UniProt 1–412 Chain E； UniProt 1–412	Mutation：A152T	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 10 ZN ZINC ION × 2	ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 2.85 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 9LEX	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8YX6 Structure of a Cys-loop Receptor in Zinc Binding State Deposited 2024-04-02	Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 1–412（412 aa） Chain B 1–412（412 aa） Chain C 1–412（412 aa） Chain D 1–412（412 aa） Chain E 1–412（412 aa）	Not recorded	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 ZN ZINC ION × 10	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
8YX7 Structure of a Cys-loop Receptor under Acidic Condition Deposited 2024-04-02	Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 1–412（412 aa） Chain B 1–412（412 aa） Chain C 1–412（412 aa） Chain D 1–412（412 aa） Chain E 1–412（412 aa）	Not recorded	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.80 Å
8YX8 Structure of a Cys-loop Receptor in Apo State Deposited 2024-04-02	Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 1–412（412 aa） Chain B 1–412（412 aa） Chain C 1–412（412 aa） Chain D 1–412（412 aa） Chain E 1–412（412 aa）	Not recorded	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.80 Å
9LET Cryo-EM structure of human ZAC in zinc Binding State Deposited 2025-01-07	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 1–412（412 aa） Chain B 1–412（412 aa） Chain C 1–412（412 aa） Chain D 1–412（412 aa） Chain E 1–412（412 aa）	Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T	ZN ZINC ION × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.62 Å
9LEU Cryo-EM structure of human ZAC with A152 mutant in zinc binding state Deposited 2025-01-07	Different ligand/ion Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 1–412（412 aa） Chain B 1–412（412 aa） Chain C 1–412（412 aa） Chain D 1–412（412 aa） Chain E 1–412（412 aa）	Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T	ZN ZINC ION × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.63 Å
9LEV Cryo-EM structure of human ZAC in nanodisc in apo state Deposited 2025-01-07	Different ligand/ion Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 1–412（412 aa） Chain B 1–412（412 aa） Chain C 1–412（412 aa） Chain D 1–412（412 aa） Chain E 1–412（412 aa）	Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.35 Å
9LEY Cryo-EM structure of human ZAC in complex with d-tubocurarine Deposited 2025-01-07	Different ligand/ion Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 1–412（412 aa） Chain B 1–412（412 aa） Chain C 1–412（412 aa） Chain D 1–412（412 aa） Chain E 1–412（412 aa）	Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T	TC9 D-TUBOCURARINE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.97 Å
9LEZ Cryo-EM structure of human ZAC in complex with N-(4-(tert-butyl)thiazol-2-yl)-3-fluorobenzamide (TTFB) Deposited 2025-01-07	Different ligand/ion Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 1–412（412 aa） Chain B 1–412（412 aa） Chain C 1–412（412 aa） Chain D 1–412（412 aa） Chain E 1–412（412 aa）	Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T Mutation：A152T	A1EKJ ~{N}-(4-~{tert}-butyl-1,3-thiazol-2-yl)-3-fluoranyl-benzamide × 5 ZN ZINC ION × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.54 Å

8 other PDB entries and 8 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	ZACN_HUMAN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–412； UniProt 1–412 Author chain B； PDBConstruct 1–412； UniProt 1–412 Author chain C； PDBConstruct 1–412； UniProt 1–412 Author chain D； PDBConstruct 1–412； UniProt 1–412 Author chain E； PDBConstruct 1–412； UniProt 1–412

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	9lex
Deposition date deposition_date	2025-01-07
Structure title title	Cryo-EM structure of human ZAC in zinc partially binding state in nanodisc
Keywords keywords	Cys-loop receptor, homopentamer, cation channel, zinc-binding state, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method method	ELECTRON MICROSCOPY

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	37.64 Å
Radius of gyration Rg (electron density) rg_electron	36.85 Å
Forward intensity I(0) i0	432389000.00
Molecular weight molecular_weight	180800.0 kDa
Excluded volume excluded_volume	231130 Å³
Envelope volume envelope_volume	295270 Å³
Hydration-shell volume shell_volume	65016 Å³
Envelope diameter envelope_diameter	123.0 Å
Shell Rg shell_rg	44.57 Å
Envelope Rg envelope_rg	36.55 Å
Shape Rg shape_rg	36.85 Å
Total Rg total_rg	37.32 Å
Total atoms total_atoms	12742
Residues n_residues	1555
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	122.3 Å
Rg (real space) rg_real	37.54 Å
Rg uncertainty (real space) rg_real_error	1.13 Å
I(0) (real space) i0_real	4.3240e+08
I(0) uncertainty (real space) i0_real_error	8.3450e+06
Rg (reciprocal space) rg_reciprocal	37.60 Å
I(0) (reciprocal space) i0_reciprocal	432400000.0000
Solution quality estimate total_estimate	0.8743
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	2
Primary peak position r_peak_primary	46.6 Å
Skewness Skewness skewness	0.325
Kurtosis Kurtosis kurtosis	-0.281
Angular range angular_range	— – 0.2100 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	71420000.0000
Real-space data points n_real_points	43
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.824; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.987; Smooth: 0.902

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Description description	Ligand-gated cation channel ZACN,Genome polyprotein
Molecular weight formula_weight	78830.9 kDa
EC 编号 ec_number	3.4.22.29,3.6.1.15,3.4.22.28,2.7.7.48
Mutation mutation	A152T
Source method src_method	man
Copy count entity_count	5

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E
Atom count atom_count	12742

MMALWSLLHLTFLGFSITLLLVHGQGFQGTAAIWPSLFNVNLSKKVQESIQIPNNGSAPLLVDVRVFVSNVFNVDILRYTMSSMLLLRLSWLDTRLAWNTSAHPRHAITLPWESLWTPRLTILEALWVDWRDQSPQARVDQDGHVKLNLALTTETNCNFELLHFPRDHSNCSLSFYALSNTAMELEFQAHVVNEIVSVKREYVVYDLKTQVPPQQLVPCFQVTLRLKNTALKSIIALLVPAEALLLADVCGGLLPLRAIERIGYKVTLLLSYLVLHSSLVQALPSSSSCNPLLIYYFTILLLLLFLSTIETVLLAGLLARGNLGAKSGPSPAPRGEQREHGNPGPHPAEEPSRGVKGSQRSWPETADRIFFLVYVVGVLCTQFVFAGIWMWAACKSDAAPGEAAPHGRRPRLSDLEVLFQGPEFWSHPQFEKGGGSGGGSGGSAWSHPQFEKEFDIDYKDDDDKSRMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK

来源 Source

来源生物 organism	Human enterovirus 71
通用名 common_name	humanEV71, EV-71
NCBI Class编号 ncbi_taxonomy_id	39054
宿主生物 host_organism	Homo sapiens

Entity 2 Entity 2 branched

Description description	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
Molecular weight formula_weight	586.5 kDa
Source method src_method	man
Copy count entity_count	10

Entity 3 Entity 3 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	2

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8. Citations (1)

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Resolution resolution	2.85 Å
Resolution测定方法 resolution_method	—
颗粒数 num_particles	111743

fit	/app/data/saxs_computation/le/9lex/9lex.fit
pddf	/app/data/pr_computation/le/9lex/9lex.pr.out
profile	/app/data/saxs_computation/le/9lex/9lex.dat

Cryo-EM structure of human ZAC in zinc partially binding state in nanodisc

1. Protein Identity and Related Structures Protein Identity & Related Structures

Ligand-gated cation channel ZACN,Genome polyprotein

State in the Current Structure

Other States of the Same Protein in the Database

Ligand-gated cation channel ZACN,Genome polyprotein

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

冷冻电镜 EM 3D

6. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

来源 Source

Entity 2 Entity 2 branched

Entity 3 Entity 3 non-polymer

8. Citations (1)

9. Files and Curves (10)

Files (7)

Curves (3)