Neutralizing monoclonal antibody Fab fragment for human leptin

entry_id	9puk
deposition_date	2025-07-31
last_revision	2025-11-05
title	Neutralizing monoclonal antibody Fab fragment for human leptin
keywords	Antibody, FAB, leptin, IMMUNE SYSTEM; IMMUNE SYSTEM
method	X-RAY DIFFRACTION

rg_guinier	30.28 Å
rg_electron	29.22 Å
i0	130420000.00
molecular_weight	88762.0 kDa
excluded_volume	110600 ų
envelope_volume	142330 ų
shell_volume	40091 ų
envelope_diameter	95.9 Å
shell_rg	37.12 Å
envelope_rg	28.84 Å
shape_rg	29.22 Å
total_rg	29.98 Å
total_atoms	12336
n_residues	842
n_harmonics	20
q_range	— – 0.5000 Å−1
n_points	101
shell_type	directional
solvent_density	0.3340 e/ų
contrast_shell	0.0300 e/ų
crysol_version	4.1.3

dmax	90.8 Å
rg_real	30.12 Å
rg_real_error	0.53 Å
i0_real	1.3040e+08
i0_real_error	1.8590e+06
rg_reciprocal	30.19 Å
i0_reciprocal	130400000.0000
total_estimate	0.9094
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	38.4 Å
skewness	0.138
kurtosis	-0.510
angular_range	— – 0.2600 Å−1
current_alpha	0.0000
highest_alpha	13450000.0000
n_real_points	53
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.967; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.989; Smooth: 0.929

Unit Cell

length_a	70.215 Å
length_b	207.941 Å
length_c	72.823 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

Symmetry

space_group	P 21 21 2
int_tables_number	18
crystal_system	orthorhombic

Refinement

resolution	3.300 Å
resolution_low	49.330 Å
r_factor	0.2726
r_work	0.2674
r_free	0.3195

Diffraction

temperature

100.0 K

Files (7)

• pr_distribution /app/data/pr_computation/pu/9puk/pr_distribution.json
• pr_output /app/data/pr_computation/pu/9puk/9puk.pr.out
• pr_plot /app/data/visualization/pr_computation/pu/9puk/9puk_pr_distribution.png
• saxs_curve_dat /app/data/saxs_computation/pu/9puk/9puk.dat
• saxs_fit /app/data/saxs_computation/pu/9puk/9puk.fit
• saxs_log /app/data/saxs_computation/pu/9puk/9puk.log
• saxs_plot /app/data/visualization/saxs_computation/pu/9puk/9puk_saxs_profile.png

Curves (3)

fit	/app/data/saxs_computation/pu/9puk/9puk.fit
pddf	/app/data/pr_computation/pu/9puk/9puk.pr.out
profile	/app/data/saxs_computation/pu/9puk/9puk.dat