SAXSdb — 9q05

▼

1. Structure Basics

entry_id	9q05
deposition_date	2025-08-12
title	syNOS monomer bound to NADPH
keywords	NOS, FAD, FMN, NADPH, C2 domain, Heme, OXIDOREDUCTASE; OXIDOREDUCTASE
method	ELECTRON MICROSCOPY

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	38.91 Å
rg_electron	38.54 Å
i0	398512000.00
molecular_weight	161890.0 kDa
excluded_volume	202210 Å³
envelope_volume	270210 Å³
shell_volume	58698 Å³
envelope_diameter	139.3 Å
shell_rg	44.29 Å
envelope_rg	37.90 Å
shape_rg	38.53 Å
total_rg	38.93 Å
total_atoms	22365
n_residues	1398
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	131.9 Å
rg_real	38.92 Å
rg_real_error	1.18 Å
i0_real	3.9850e+08
i0_real_error	6.7960e+06
rg_reciprocal	38.92 Å
i0_reciprocal	398500000.0000
total_estimate	0.8784
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	45.0 Å
skewness	0.371
kurtosis	-0.270
angular_range	— – 0.2050 Å⁻¹
current_alpha	0.0000
highest_alpha	86850000.0000
n_real_points	42
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.840; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.896

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (5)

Entity 1 polymer

description	Nitric oxide synthase
formula_weight	166440.3 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	22365

MLVNDSRPTVEAHVLSVVRLVELCASGIPSNNEFKYKANVRVTCSGTEQSNTQLMTRLQPSWLVDIAHPSNCLFTVTLFYRQGGLGQPWHEAGSIKVTTADLFDKQRSVEISRPVATWPAAPELMLNARFTCSDHTSQSGEAVSLSLAGTRANASRRPTSLALVSDDSIELPEAIPLTYSEAVIVKDVWNKLRAWKELQMETFFKRLLLEVPELDYIFGEAFESIPDYFFEMFDCCVRELCPHTENVVWEPMMGVPPEKGDAFDTVADYGALFADIGMQPQHWLRARQVWMWMLPQIPYLEEYDREDLAKGNKSALCKFFNTHVIGGMVAARDRYDSALPPALVQKMADSWQYFAPRKNEMGVEFYQTLFERYPQVLPIFGRADMDYLSTHLFQSLEFIFLCLAEGSTERLMKELRHLGRLHGNAGVPSFAYGAISEVMISMFEKYVPGFDEQLKEAWQVLIARVSNVIKLPKLNEERLLKKAREYLDVIANEQAWEESDRERRWQEIKAEVQATGTYTHTYEELAYGAQLAWRNTSKCIGRIQWSNMVVRDRRHVTDPDEMFQELEEHLRLGTNGGNIQIVMTVFRPKLPKERWGPRIWNPQLIRYAAYEMPDGSIMGDAANLELTHQIIEKMGWQPPEPRSPYDILPLVIEVPRHEPRLYSFAPEEILEVEIEHPTIPDFKTLGLRWYAVPAISNFRMDIGGVTYACLPFNGWYMGTEIARDFLEGGRYGKMKAIANLLGLNTSSEQTLWRDRVALEMNIAVLHSFQKAKVTMVDHQSAAQQFLAHDLREKRAGRECPADYGWVVPPAGGSACPVWHHQMRDFYLEPAYHHAADRWAVEADIDLEQFVQTTHESDHQRDRILILFGSETGTAEGFARRAARQLSAYHPKVMALDDYNVNTLDEEKLLLVVTSTFGNGEVPGNAQQFTQWLKQQPSDTLNGLNYSVLGIGSTVYEHFCAAGITLDKALAKAGANSVVPLHKGDEIKGQADTFKRWLSLISRILGADSTSTTPTTSKLKVTYLADSESHALLNLEAEHSHSRVPVLTNQELLKAVTPGSRSTRYLLFDTAKTEIAYETGDHVSVHPHNPEELVLRVCDRLSLSPDTAFSAKYVLPDGRQLEDEPPIAVPTTVGQALTEDLDLAFKEPFGELLNVLHQAAENTEEKIRLETWLEILALEDGHEENAALRKMLRDNFMSVADLFDEFPSAQITLEMLLEVLPKEKPRLYSISSCPQLQPGKLQITVGVLQIQTDAGKTRQGLCSNYLAGLSEGDLVRIETHTSDFRPPNDPSAPLLMVGPGTGISPLIAFLQHREYLNSQGIPLGKATLYTGCRNHDDFLYEDQLRVWLEQGTLTDLQVAFSRLTAQKVYVQNLMQDNARSLWQQLSHSQCHYYVCGDAKMADNVFEVFMQIAKTEGGLTHLEAVDFFNRMKSEKRFSTDVWGVTLNFKQAIKQVEKDNYARAEKWLANL

Source

organism	Synechococcus sp. PCC 7335
ncbi_taxonomy_id	91464
host_organism	Escherichia coli BL21(DE3)

Entity 2 non-polymer

description	PROTOPORPHYRIN IX CONTAINING FE
formula_weight	616.5 kDa
src_method	syn
entity_count	2

Entity 3 non-polymer

description	FLAVIN-ADENINE DINUCLEOTIDE
formula_weight	785.5 kDa
src_method	syn
entity_count	1

Entity 4 non-polymer

description	FLAVIN MONONUCLEOTIDE
formula_weight	456.3 kDa
src_method	syn
entity_count	1

Entity 5 non-polymer

description	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
formula_weight	743.4 kDa
src_method	syn
entity_count	1

▶

6. Citations (1)

▶

resolution	3.090 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	3.09 Å
resolution_method	—
num_particles	45964

fit	/app/data/saxs_computation/q0/9q05/9q05.fit
pddf	/app/data/pr_computation/q0/9q05/9q05.pr.out
profile	/app/data/saxs_computation/q0/9q05/9q05.dat

syNOS monomer bound to NADPH

SAXS Profile

P(r) Distribution