GH161 phosphorylase in complex with beta-1,3 glucooligosaccharide

Method: ELECTRON MICROSCOPY D_max: 138.1 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9qqk
deposition_date	2025-04-01
last_revision	2025-12-10
title	GH161 phosphorylase in complex with beta-1,3 glucooligosaccharide
keywords	phosphorylase, gh161, laminarin, CYTOSOLIC PROTEIN; CYTOSOLIC PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	42.18 Å
rg_electron	41.75 Å
i0	825217000.00
molecular_weight	238060.0 kDa
excluded_volume	298100 Å³
envelope_volume	396420 Å³
shell_volume	76412 Å³
envelope_diameter	145.3 Å
shell_rg	49.20 Å
envelope_rg	41.16 Å
shape_rg	41.74 Å
total_rg	42.12 Å
total_atoms	16766
n_residues	2076
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	138.1 Å
rg_real	42.10 Å
rg_real_error	1.49 Å
i0_real	8.2520e+08
i0_real_error	1.6290e+07
rg_reciprocal	42.18 Å
i0_reciprocal	825300000.0000
total_estimate	0.8821
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	52.2 Å
skewness	0.289
kurtosis	-0.317
angular_range	— – 0.1850 Å⁻¹
current_alpha	0.0000
highest_alpha	212500000.0000
n_real_points	38
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.878; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.992; Smooth: 0.837

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	gh161 phosphorylase
formula_weight	119868.0 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	16766

MTGKNETFVMDDYGKKSTFASFLPGIAGIRGIPIWCYYVNRGQCVVSFGVDNKDHAIMEFYPAHQAYQNVKTTGFRTFLKKNGTVFEPFSDENITHRMQIHMNGLAIEEQNRSSGMDTKVVYYTLPGENVGALVRVVSVTNQSGEPIELELIDGMPAVIPYGVSMDSMKNMGQTAKAWMQVEDLSEGLPYYRVRASMDDTAAVRRIDGGNFSACCEADGRRLQPIVDPSLIFSYDLSLKRPVGFEERPLKELLLEEQMTQNLLPCSFYGITRTLAPGGSVTLYELIGQVENKQLLKEYFAEKKDAAYFEAKKREADELAEALTDGIRTRTASAAFDAYCRYTYMDNVLRGGYPMQLGNNKIFYVYSRKHGDLERDYNYFSMLPEFYSQGNGNFRDVNQNRRCDTFFAPFVGRKNIQEFYSLIQLDGYNPLGVEKLTYRLSKERAKKLLTDVKEEQRSALIDFATKPFTPGALCRKFGEVFGDTWDETLFIRVIDFAEEMVNGSFGEGYWSDHWTYNLDLILDYLSVFPEQEKEMLYEEVYTTFLSRINVNRRFRRYVETENGLRQYRALNEASRRADLGEKLVRTEYGSGDVLTMTLMEKLILLGAVKFATLDAYGMGIEMEGGKPGWYDALNGMPGLFGSSMAETYELARMLSYTIEALKQYPGEVALIEELGCFLDELNLITRLEHDNIMRDEELLSFWNRINDAKEIYRDKTYQGVSGKKMVYHTEQLAAILEGFLEIVTCGIKKARRISGEICPTYFTYEVPEYEKLKDGGIRPLKFVPQNMPYFLEGPVRYLKLPVEQGEKRALYEAVKESDLYDGELSMYKVNASLADSSFELGRARAFTPGWLENESIWLHMEYKYLLELLRSGLYEEFFADFKKAAIPFQNPEIYGRSIYENSSFIASSRNPNPSCRGRGFVARLSGSTIEFISMWKEMMFGAHPFRTEQEELVFSLAPAIPAYLIPEDGRLSAAFMSKTTVCYEFGGHRDYVPGTYRIRHMVFFYENGSQATVEGEKVSGKLAEDIRAGRVRKMEVAVDLEHHHHHH

Source

organism	gut metagenome
ncbi_taxonomy_id	749906
host_organism	Escherichia coli BL21(DE3)

Entity 2 branched

description	beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose
formula_weight	828.7 kDa
src_method	man
entity_count	2

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	3.14 Å
resolution_method	—
num_particles	22729

fit	/app/data/saxs_computation/qq/9qqk/9qqk.fit
pddf	/app/data/pr_computation/qq/9qqk/9qqk.pr.out
profile	/app/data/saxs_computation/qq/9qqk/9qqk.dat