Structure and mechanism of the broad spectrum CRISPR-associated ring nuclease Crn4

entry_id	9qs9
deposition_date	2025-04-04
title	Structure and mechanism of the broad spectrum CRISPR-associated ring nuclease Crn4
keywords	CRISPR, Ring Nuclease, cyclic oligoadenylate, Phage, viral protein, CELL INVASION; CELL INVASION
method	X-RAY DIFFRACTION

rg_guinier	31.39 Å
rg_electron	31.09 Å
i0	124785000.00
molecular_weight	85693.0 kDa
excluded_volume	106130 ų
envelope_volume	138070 ų
shell_volume	37655 ų
envelope_diameter	107.3 Å
shell_rg	37.26 Å
envelope_rg	30.99 Å
shape_rg	31.10 Å
total_rg	31.58 Å
total_atoms	6029
n_residues	766
n_harmonics	20
q_range	— – 0.5000 Å−1
n_points	101
shell_type	directional
solvent_density	0.3340 e/ų
contrast_shell	0.0300 e/ų
crysol_version	4.1.3

dmax	105.2 Å
rg_real	31.43 Å
rg_real_error	1.26 Å
i0_real	1.2480e+08
i0_real_error	2.4610e+06
rg_reciprocal	31.42 Å
i0_reciprocal	124800000.0000
total_estimate	0.8788
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	40.0 Å
skewness	0.375
kurtosis	-0.285
angular_range	— – 0.2500 Å−1
current_alpha	0.0000
highest_alpha	84350000.0000
n_real_points	51
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.856; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.982; Smooth: 0.872

Unit Cell

length_a	66.271 Å
length_b	102.899 Å
length_c	72.442 Å
angle_alpha	90.00 °
angle_beta	103.88 °
angle_gamma	90.00 °
z_pdb	12

Symmetry

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

Refinement

resolution	1.441 Å
resolution_low	70.425 Å
r_factor	—
r_work	0.2220
r_free	0.2395

Diffraction

temperature

100.0 K

Files (7)

• pr_distribution /app/data/pr_computation/qs/9qs9/pr_distribution.json
• pr_output /app/data/pr_computation/qs/9qs9/9qs9.pr.out
• pr_plot /app/data/visualization/pr_computation/qs/9qs9/9qs9_pr_distribution.png
• saxs_curve_dat /app/data/saxs_computation/qs/9qs9/9qs9.dat
• saxs_fit /app/data/saxs_computation/qs/9qs9/9qs9.fit
• saxs_log /app/data/saxs_computation/qs/9qs9/9qs9.log
• saxs_plot /app/data/visualization/saxs_computation/qs/9qs9/9qs9_saxs_profile.png

Curves (3)

fit	/app/data/saxs_computation/qs/9qs9/9qs9.fit
pddf	/app/data/pr_computation/qs/9qs9/9qs9.pr.out
profile	/app/data/saxs_computation/qs/9qs9/9qs9.dat