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9r8p

Cryo-EM structure of hSA3, a mutated hyper-soluble human serum albumin designed for bacterial expression

Method: ELECTRON MICROSCOPY Dmax: 90.2 Å Quality: EXCELLENT

SAXS Profile

SAXS profile for 9r8p

P(r) Distribution

P(r) distribution for 9r8p

1. Structure Basics

entry_id9r8p
deposition_date2025-05-16
titleCryo-EM structure of hSA3, a mutated hyper-soluble human serum albumin designed for bacterial expression
keywordsligand and peptide binding protein, PROTEIN TRANSPORT; PROTEIN TRANSPORT
methodELECTRON MICROSCOPY

Download Data

2. SAXS Parameters (CRYSOL)

rg_guinier29.08
rg_electron28.03
i076037800.00
molecular_weight67355.0 kDa
excluded_volume84117 ų
envelope_volume114640 ų
shell_volume34491 ų
envelope_diameter94.2
shell_rg34.98
envelope_rg27.83
shape_rg28.03
total_rg28.79
total_atoms4714
n_residues582
n_harmonics20
q_range— – 0.5000 −1
n_points101
shell_typedirectional
solvent_density0.3340 e/ų
contrast_shell0.0300 e/ų
crysol_version4.1.3

3. P(r) Analysis (GNOM)

dmax90.2
rg_real28.99
rg_real_error0.60
i0_real7.6040e+07
i0_real_error9.3570e+05
rg_reciprocal29.03
i0_reciprocal76040000.0000
total_estimate0.9078
solution_quality EXCELLENT a EXCELLENT solution
n_peaks1
r_peak_primary34.4
skewness0.206
kurtosis-0.512
angular_range— – 0.2750 −1
current_alpha0.0000
highest_alpha13910000.0000
n_real_points56
gnom_version4.1.3
quality_criteria AN1: 0.000; Oscil: 0.955; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.933

4. Crystallography & Experiment

5. Entities & Polymer Info (1)

6. Citations (1)

7. Files & Curves (10)