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9r9n

Cryo-EM structure of a contractile injection system in Salmonella enterica subspecies Salamae, the cap portion in extended state.

Method: ELECTRON MICROSCOPY D_max: 159.2 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9r9n
deposition_date	2025-05-20
title	Cryo-EM structure of a contractile injection system in Salmonella enterica subspecies Salamae, the cap portion in extended state.
keywords	contractile injection system Salmonella, CONTRACTILE PROTEIN; CONTRACTILE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	42.11 Å
rg_electron	42.25 Å
i0	220922000.00
molecular_weight	122660.0 kDa
excluded_volume	154410 Å³
envelope_volume	227460 Å³
shell_volume	48362 Å³
envelope_diameter	170.8 Å
shell_rg	43.36 Å
envelope_rg	42.29 Å
shape_rg	42.26 Å
total_rg	42.30 Å
total_atoms	17243
n_residues	1102
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	159.2 Å
rg_real	42.51 Å
rg_real_error	1.96 Å
i0_real	2.2090e+08
i0_real_error	3.9830e+06
rg_reciprocal	42.12 Å
i0_reciprocal	220800000.0000
total_estimate	0.8052
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	43.0 Å
skewness	0.618
kurtosis	-0.002
angular_range	— – 0.1850 Å⁻¹
current_alpha	0.0000
highest_alpha	24040000.0000
n_real_points	38
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.602; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.730; Smooth: 0.927

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	Phage tail sheath family protein
formula_weight	57351.9 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	17243

MGVMKTPGVYIVEQNAFPNSVVEVATAVPAFIGYTEKADNGGKSLSNKGWRITSMSEYRQYFGGEPQHLFEISEISTTSNANIREAFKQSGKTYQITQSNTRHHLYYSMLFFFQNGGGPCYIVSVGNYSDDIDAAVLKGGILPLIKEAEPTMLLIPEAIQLAEDDCINVEQAMLGHCGGKMKNRVAILDVWNGYKDRQHPDGDCVESFRSKLGTHYLDYAAAYYPWLNTSIVQDSDVSFLNISNIDKLAELLSGEVALMFSDLEGLSEEELSTGGNKLRATRKQAMLDEIAKLSAEISRPDAVLLHKILSNMSPLYQTIMADIKFQQNILPPSSAMAGIYTMVDNSRGVWKAPANVSVNAVVSPTVNISDDEQEDLNVTTQGKSINAIRPFIGEGTLVWGARTLDGNSVDWRYINVRRTMIMLEESIKLASKAYVFEPNVANTWVSMESMLSNFLYGIWKRGGLAGSTPGEAYNVSVGLGKTMTSNDILEGILRITVLVAMVRPAEFIEITFQQKMQES

Source

organism	Salmonella enterica subsp. salamae
ncbi_taxonomy_id	59202
host_organism	Escherichia coli

Entity 2 polymer

description	DUF4255 domain-containing protein
formula_weight	22038.9 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

MLDLCLNYLKERMNQSVKNVFDLADDLVIVSPPTDLDGSKLPKIQNKILIFISNIEKDSFSKSYRQDFNHTSNRTAVSSQPLFITITVTVAANFSTNHYSDGLKVLSHFLAFFNRHNSFNRQNSPDLPKNIEQLNMELDSIPGDQLNHLWGIFGSHYLPSCTYRVRALIPDSESILTQVGNIHLSDTTLAKRDN

Source

organism	Salmonella enterica subsp. salamae
ncbi_taxonomy_id	59202
host_organism	Escherichia coli

Entity 3 polymer

description	Phage tail protein
formula_weight	17091.3 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	D

MADDGSAQSDTVWPMPKFYFEVKWDGGAGAEMVSAFQEVSGLDSEAQPIEYRAGNSPVFSTIKMPGLIKSGNVTLKKGTFKGDNKFYEWYSKIKMNTIARTAVTINLLDESGAPVMSWKLKNAWPTKVTGTDLKSDSNEVAVETIELAHEGLEISV

Source

organism	Salmonella enterica subsp. salamae
ncbi_taxonomy_id	59202
host_organism	Escherichia coli

Entity 4 polymer

description	SalCis16b - cap
formula_weight	29448.8 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	C

MADYQTILTISVLHEYYNASSDKFAPIGLVADRETVLLLRQYGILLKSARGFTRLIVDTVRYSDLADLTAELTFRFYLVSTDPGFRNITKMPDMFDISILNAEFTDSSELNITAEHWVDVNQLNTSTAIDSAVIHNKNFIGLLTISLPKSHCTLEKKNITVRFNAISAYWKYYIFSPGGKKNLNIPHSFTEQEPEQVANKTARIFMSDNPILLRKIYAEPFSLLDANNVIIKSLPLPMPDNISTSIVKGFKITIAHIYIN

Source

organism	Salmonella enterica subsp. salamae
ncbi_taxonomy_id	59202
host_organism	Escherichia coli

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6. Citations (1)

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resolution	2.86 Å
resolution_method	—
num_particles	5471

fit	/app/data/saxs_computation/r9/9r9n/9r9n.fit
pddf	/app/data/pr_computation/r9/9r9n/9r9n.pr.out
profile	/app/data/saxs_computation/r9/9r9n/9r9n.dat

Cryo-EM structure of a contractile injection system in Salmonella enterica subspecies Salamae, the cap portion in extended state.

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

EM 3D

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)