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9sgk

F-actin in complex with USP54 M1 actin binding motif

Method: ELECTRON MICROSCOPY D_max: 175.9 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9sgk
deposition_date	2025-08-22
title	F-actin in complex with USP54 M1 actin binding motif
keywords	Actin, STRUCTURAL PROTEIN; STRUCTURAL PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	48.22 Å
rg_electron	48.80 Å
i0	702905000.00
molecular_weight	216870.0 kDa
excluded_volume	270550 Å³
envelope_volume	376110 Å³
shell_volume	68346 Å³
envelope_diameter	189.1 Å
shell_rg	48.38 Å
envelope_rg	48.51 Å
shape_rg	48.81 Å
total_rg	48.75 Å
total_atoms	30205
n_residues	1915
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	175.9 Å
rg_real	48.79 Å
rg_real_error	2.08 Å
i0_real	7.0290e+08
i0_real_error	1.5890e+07
rg_reciprocal	48.22 Å
i0_reciprocal	702400000.0000
total_estimate	0.8148
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	48.0 Å
skewness	0.614
kurtosis	-0.101
angular_range	— – 0.1650 Å⁻¹
current_alpha	0.0000
highest_alpha	88660000.0000
n_real_points	34
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.631; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.886; Smooth: 0.809

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	Actin binding motif
formula_weight	33529.5 kDa
src_method	man
entity_count	5

Polymer

type	polypeptide(L)
strand_id	B,C,D,E,P
atom_count	30205

MIPSTKGSVRSLIEQFERIQGVSAGDPPVATSVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKADENLYFQGGGSGGSSKHHHHSGHHHTGHHH

Source

organism	Homo sapiens
ncbi_taxonomy_id	9606
host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

description	Actin, alpha skeletal muscle
formula_weight	42110.0 kDa
ec_number	3.6.4.-
src_method	nat
entity_count	5

Polymer

type	polypeptide(L)
strand_id	F,G,H,I,J

MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF

Entity 3 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	5

Entity 4 non-polymer

description	ADENOSINE-5'-DIPHOSPHATE
formula_weight	427.2 kDa
src_method	syn
entity_count	5

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6. Citations (1)

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resolution	3.80 Å
resolution_method	—
num_particles	111239

fit	/app/data/saxs_computation/sg/9sgk/9sgk.fit
pddf	/app/data/pr_computation/sg/9sgk/9sgk.pr.out
profile	/app/data/saxs_computation/sg/9sgk/9sgk.dat

F-actin in complex with USP54 M1 actin binding motif

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

EM 3D

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Entity 3 non-polymer

Entity 4 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)