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9sr2

Catalytically active GH161A phosphorylase refined in C1

Method: ELECTRON MICROSCOPY D_max: 136.2 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9sr2
deposition_date	2025-09-23
last_revision	2025-12-10
title	Catalytically active GH161A phosphorylase refined in C1
keywords	GH161, phosphorylase, HYDROLASE; HYDROLASE
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	41.55 Å
rg_electron	41.07 Å
i0	829001000.00
molecular_weight	237910.0 kDa
excluded_volume	297690 Å³
envelope_volume	393850 Å³
shell_volume	76874 Å³
envelope_diameter	144.2 Å
shell_rg	48.98 Å
envelope_rg	40.54 Å
shape_rg	41.07 Å
total_rg	41.47 Å
total_atoms	16754
n_residues	2076
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	136.2 Å
rg_real	41.45 Å
rg_real_error	0.75 Å
i0_real	8.2900e+08
i0_real_error	1.3450e+07
rg_reciprocal	41.55 Å
i0_reciprocal	829100000.0000
total_estimate	0.8805
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	53.8 Å
skewness	0.281
kurtosis	-0.282
angular_range	— – 0.1900 Å⁻¹
current_alpha	0.0002
highest_alpha	232800000.0000
n_real_points	39
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.866; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.985; Smooth: 0.858

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	GH161A
formula_weight	119868.0 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	16765

MTGKNETFVMDDYGKKSTFASFLPGIAGIRGIPIWCYYVNRGQCVVSFGVDNKDHAIMEFYPAHQAYQNVKTTGFRTFLKKNGTVFEPFSDENITHRMQIHMNGLAIEEQNRSSGMDTKVVYYTLPGENVGALVRVVSVTNQSGEPIELELIDGMPAVIPYGVSMDSMKNMGQTAKAWMQVEDLSEGLPYYRVRASMDDTAAVRRIDGGNFSACCEADGRRLQPIVDPSLIFSYDLSLKRPVGFEERPLKELLLEEQMTQNLLPCSFYGITRTLAPGGSVTLYELIGQVENKQLLKEYFAEKKDAAYFEAKKREADELAEALTDGIRTRTASAAFDAYCRYTYMDNVLRGGYPMQLGNNKIFYVYSRKHGDLERDYNYFSMLPEFYSQGNGNFRDVNQNRRCDTFFAPFVGRKNIQEFYSLIQLDGYNPLGVEKLTYRLSKERAKKLLTDVKEEQRSALIDFATKPFTPGALCRKFGEVFGDTWDETLFIRVIDFAEEMVNGSFGEGYWSDHWTYNLDLILDYLSVFPEQEKEMLYEEVYTTFLSRINVNRRFRRYVETENGLRQYRALNEASRRADLGEKLVRTEYGSGDVLTMTLMEKLILLGAVKFATLDAYGMGIEMEGGKPGWYDALNGMPGLFGSSMAETYELARMLSYTIEALKQYPGEVALIEELGCFLDELNLITRLEHDNIMRDEELLSFWNRINDAKEIYRDKTYQGVSGKKMVYHTEQLAAILEGFLEIVTCGIKKARRISGEICPTYFTYEVPEYEKLKDGGIRPLKFVPQNMPYFLEGPVRYLKLPVEQGEKRALYEAVKESDLYDGELSMYKVNASLADSSFELGRARAFTPGWLENESIWLHMEYKYLLELLRSGLYEEFFADFKKAAIPFQNPEIYGRSIYENSSFIASSRNPNPSCRGRGFVARLSGSTIEFISMWKEMMFGAHPFRTEQEELVFSLAPAIPAYLIPEDGRLSAAFMSKTTVCYEFGGHRDYVPGTYRIRHMVFFYENGSQATVEGEKVSGKLAEDIRAGRVRKMEVAVDLEHHHHHH

Source

organism	gut metagenome
ncbi_taxonomy_id	749906
host_organism	Escherichia coli BL21(DE3)

Entity 2 branched

description	beta-D-glucopyranose-(1-3)-beta-D-glucopyranose
formula_weight	342.3 kDa
src_method	man
entity_count	2

Entity 3 non-polymer

description	PHOSPHATE ION
formula_weight	95.0 kDa
src_method	syn
entity_count	2

Entity 4 non-polymer

description	beta-D-glucopyranose
formula_weight	180.2 kDa
src_method	syn
entity_count	2

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	2.65 Å
resolution_method	—
num_particles	179478

fit	/app/data/saxs_computation/sr/9sr2/9sr2.fit
pddf	/app/data/pr_computation/sr/9sr2/9sr2.pr.out
profile	/app/data/saxs_computation/sr/9sr2/9sr2.dat