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9tbe

Cryo-EM structure of the light-driven sodium pump ErNaR in the monomeric form in the K2 state

Method: ELECTRON MICROSCOPY D_max: 74.1 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9tbe
deposition_date	2025-11-19
title	Cryo-EM structure of the light-driven sodium pump ErNaR in the monomeric form in the K2 state
keywords	rhodopsin, ErNaR, sodium pump, phototransduction, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	21.61 Å
rg_electron	20.45 Å
i0	13841300.00
molecular_weight	33308.0 kDa
excluded_volume	43941 Å³
envelope_volume	48672 Å³
shell_volume	20270 Å³
envelope_diameter	75.7 Å
shell_rg	26.81 Å
envelope_rg	20.89 Å
shape_rg	20.45 Å
total_rg	21.46 Å
total_atoms	2360
n_residues	272
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	74.1 Å
rg_real	21.72 Å
rg_real_error	0.63 Å
i0_real	1.3840e+07
i0_real_error	1.9070e+05
rg_reciprocal	21.70 Å
i0_reciprocal	13840000.0000
total_estimate	0.8565
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	20.7 Å
skewness	0.448
kurtosis	-0.383
angular_range	— – 0.3700 Å⁻¹
current_alpha	0.0000
highest_alpha	3663000.0000
n_real_points	69
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.770; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.828; Smooth: 0.992

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	Bacteriorhodopsin-like protein
formula_weight	30532.3 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	D
atom_count	2380

MPSIENFLAYDFWQYDVIRHLFAFSTAVFLAGLVYFAMTARTTAPNYRLSANISAVVMVSAALELGQLWLLWNESFQWAELQGSFVPVAGERFSNGYRYMNWLIDVPMLATQLVVVCGFVGTELRNRWAKLTIAGVLMILTGYVGQYFEPAVAGVPGYEGAEQFWIWGIISTAFFVWMLLILANAVRNPQGAPSDEVRSRLKFCFWFLLATWSIYPFAYAMPLFAPTADGVVVRQVIYTVADVSSKLVFGVILSQVALRRSAEEGFEPARVA

Source

organism	Erythrobacter
ncbi_taxonomy_id	1041
host_organism	Escherichia coli

Entity 2 non-polymer

description	EICOSANE
formula_weight	282.5 kDa
src_method	syn
entity_count	11

Entity 3 non-polymer

description	DODECYL-BETA-D-MALTOSIDE
formula_weight	510.6 kDa
src_method	syn
entity_count	2

Entity 4 non-polymer

description	RETINAL
formula_weight	284.4 kDa
src_method	syn
entity_count	1

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	20

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.800 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	2.80 Å
resolution_method	—
num_particles	44989

fit	/app/data/saxs_computation/tb/9tbe/9tbe.fit
pddf	/app/data/pr_computation/tb/9tbe/9tbe.pr.out
profile	/app/data/saxs_computation/tb/9tbe/9tbe.dat