← Back to search

9u5b

T200H Carbonic Anhydrase II pH 7.8 5 atm CO2

Method: X-RAY DIFFRACTION D_max: 62.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	9u5b
deposition_date	2025-03-21
last_revision	2025-07-16
title	T200H Carbonic Anhydrase II pH 7.8 5 atm CO2
keywords	Metal Binding Protein, LYASE; LYASE
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	18.55 Å
rg_electron	17.28 Å
i0	14587200.00
molecular_weight	29035.0 kDa
excluded_volume	36448 Å³
envelope_volume	40471 Å³
shell_volume	19004 Å³
envelope_diameter	60.3 Å
shell_rg	24.04 Å
envelope_rg	17.66 Å
shape_rg	17.25 Å
total_rg	18.39 Å
total_atoms	2056
n_residues	257
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	62.5 Å
rg_real	18.40 Å
rg_real_error	0.40 Å
i0_real	1.4590e+07
i0_real_error	1.6960e+05
rg_reciprocal	18.43 Å
i0_reciprocal	14590000.0000
total_estimate	0.7897
solution_quality	GOOD a GOOD solution
n_peaks	3
r_peak_primary	24.0 Å
skewness	0.070
kurtosis	-0.455
angular_range	— – 0.4300 Å⁻¹
current_alpha	0.0000
highest_alpha	2732000.0000
n_real_points	74
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.755; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.997; Smooth: 0.000

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (4)

Entity 1 polymer

description	Carbonic anhydrase 2
formula_weight	30155.0 kDa
ec_number	4.2.1.1,4.2.1.69
mutation	T200H
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	2383

MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEFDDSQDKAVLKGGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESLDYWTYPGSLTHPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASFKHHHHHH

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 2 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	1

Entity 3 non-polymer

description	BICARBONATE ION
formula_weight	61.0 kDa
src_method	syn
entity_count	1

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	263

▶

6. Citations (1)

▶

length_a	42.161 Å
length_b	41.401 Å
length_c	72.682 Å
angle_alpha	90.00 °
angle_beta	104.62 °
angle_gamma	90.00 °
z_pdb	2

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	1.250 Å
resolution_low	29.058 Å
r_factor	—
r_work	0.1432
r_free	0.1704

fit	/app/data/saxs_computation/u5/9u5b/9u5b.fit
pddf	/app/data/pr_computation/u5/9u5b/9u5b.pr.out
profile	/app/data/saxs_computation/u5/9u5b/9u5b.dat

T200H Carbonic Anhydrase II pH 7.8 5 atm CO2

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction