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9ua0

Cryo-EM structure of cyanobacterial bicarbonate transporter CmpBCD in the presence of nitrate

Method: ELECTRON MICROSCOPY D_max: 110.9 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9ua0
deposition_date	2025-03-31
title	Cryo-EM structure of cyanobacterial bicarbonate transporter CmpBCD in the presence of nitrate
keywords	ABC transporter, bicarbonate importer, membrane transporter, nitrate binding, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	35.84 Å
rg_electron	35.05 Å
i0	172492000.00
molecular_weight	114000.0 kDa
excluded_volume	146510 Å³
envelope_volume	198110 Å³
shell_volume	47332 Å³
envelope_diameter	107.0 Å
shell_rg	41.95 Å
envelope_rg	33.83 Å
shape_rg	35.05 Å
total_rg	35.59 Å
total_atoms	16410
n_residues	1027
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	110.9 Å
rg_real	35.78 Å
rg_real_error	0.78 Å
i0_real	1.7250e+08
i0_real_error	2.8160e+06
rg_reciprocal	35.82 Å
i0_reciprocal	172500000.0000
total_estimate	0.9101
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	37.8 Å
skewness	0.206
kurtosis	-0.684
angular_range	— – 0.2200 Å⁻¹
current_alpha	0.0000
highest_alpha	31850000.0000
n_real_points	45
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.970; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.997; Smooth: 0.920

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	CmpB
formula_weight	30966.5 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	16410

MAASIGNRNGSFGRSLSKFWEKNAQNWALPIVGILGFLIVWQLLTWTGLLKLPGPWDIMAEKSTRNLLLYPFFDRGGTDKGLFWQTLASFERVAKGYSIAAIVGISVGILVGTNAVIDKALDPLFQFLRTVPPLAWVPIALAALRQNEPAALFVIFITAVWPILLNTAVGVKQIPQDYRNVSRVLQLSKQKYFFKILIPSALPYIFTGLRISIGLAWLAIIAAEIIMSGIVGIGFFIWNSYTNDKVGEVILALVYIGAVGLILDRAVAWLQNVILPEEQK

Source

organism	Pseudanabaena sp. Chao 1811
ncbi_taxonomy_id	2963092
host_organism	Escherichia coli

Entity 2 polymer

description	CmpC
formula_weight	76934.0 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	C

MQDFVLVDQVERTFPLGGGKEYIALKGIDLQIRKGEFISLIGHSGCGKSTLLNMIAGLDLPSGGVVMLEDERVTRPGPDRMVVFQNYSLLPWLSVRENVALAVDEVLSHLPKGERHDLVERYVNMVGLAHAIDKPPTQLSGGMRQRVAIARALAVRPKLLLLDEPFGALDALTRGNLQEKLMQICNEDQITAVMVTHDVDEAVLLSDRIVMLTNGPASKIGNILEVDIPRPRQRMEAVNHPSYYSLRSEIIYFLNQQKRVKKLNARKVTTVAKHGLEKVNLEIGFVPLTACAPIAVAKEKGFFEKHGLDEVSLVRETSWRGIVDGISGGYLDAAQMPSGLPMWLTLGGAGACKPIVTALTMTRNGNAITLDKHFYDRGIHTLADFKAMLQSTRDRQHRMGVVHPSSMHNLLLRYWLAAGGIDPDLDVELKTIPPAQMVVDLKAGSIDGFCVGEPWNFRAAMENIGFTVATDLEIWNGHPGKVLGVREDWANAYPNTHIALVKALLEACMYCADPNNAEEIRQILSRRAYVSTNVNYIHLGNPDDVTCALDTPMREPAHHQFYGAGVNRPSRSEHLWHITQMARWGDVPFPRNWVEVLEKTCRVGVFSTAARELGLTDITYSRNAIQLFDDIPFNAEDPIGYLNHLDIKRNITMAEIAMESVRMVASSGDYKDHDGDYKDHDIDYKDDDDK

Source

organism	Pseudanabaena sp. Chao 1811
ncbi_taxonomy_id	2963092
host_organism	Escherichia coli

Entity 3 polymer

description	CmpD
formula_weight	31282.1 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	D

MTQTILEHTNQIAQIPPQESFLRIENVSKIYPTPKGEYVVLKDVNLHIQEGEFICLIGHSGCGKSTLLNMVGGFSKPSIGEVTVNGKLITKPGPDRMVVFQGYALLPWLTVYENVMLAVDSVNPNLSKSEKNDIVRHHLAMVGLSEAAEKKPTQISGGMKQRVSIARALAIRPEVLILDEPFGALDAITKEELQEELLQIWTEHRCTVLMITHDIDEALFLADRLVMMTNGPAANIGEIMRIPFPRPRDRAQIMEDPLYYDLRNTALDFLYNRFAHDE

Source

organism	Pseudanabaena sp. Chao 1811
ncbi_taxonomy_id	2963092
host_organism	Escherichia coli

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.390 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	3.39 Å
resolution_method	—
num_particles	97237

fit	/app/data/saxs_computation/ua/9ua0/9ua0.fit
pddf	/app/data/pr_computation/ua/9ua0/9ua0.pr.out
profile	/app/data/saxs_computation/ua/9ua0/9ua0.dat

Cryo-EM structure of cyanobacterial bicarbonate transporter CmpBCD in the presence of nitrate

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)