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9ua1

Cryo-EM structure of cyanobacterial bicarbonate transporter CmpBCD in complex with ATP

Method: ELECTRON MICROSCOPY D_max: 112.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9ua1
deposition_date	2025-03-31
title	Cryo-EM structure of cyanobacterial bicarbonate transporter CmpBCD in complex with ATP
keywords	ABC transporter, bicarbonate importer, membrane transporter, nitrate binding, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	34.46 Å
rg_electron	33.96 Å
i0	170879000.00
molecular_weight	112700.0 kDa
excluded_volume	144480 Å³
envelope_volume	180870 Å³
shell_volume	44654 Å³
envelope_diameter	113.5 Å
shell_rg	40.47 Å
envelope_rg	33.70 Å
shape_rg	33.98 Å
total_rg	34.38 Å
total_atoms	7947
n_residues	1008
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	112.8 Å
rg_real	34.54 Å
rg_real_error	0.80 Å
i0_real	1.7090e+08
i0_real_error	2.9310e+06
rg_reciprocal	34.49 Å
i0_reciprocal	170900000.0000
total_estimate	0.8808
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	34.3 Å
skewness	0.382
kurtosis	-0.553
angular_range	— – 0.2300 Å⁻¹
current_alpha	0.0000
highest_alpha	44140000.0000
n_real_points	47
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.849; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.981; Smooth: 0.919

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	CmpB
formula_weight	30966.5 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	7947

MAASIGNRNGSFGRSLSKFWEKNAQNWALPIVGILGFLIVWQLLTWTGLLKLPGPWDIMAEKSTRNLLLYPFFDRGGTDKGLFWQTLASFERVAKGYSIAAIVGISVGILVGTNAVIDKALDPLFQFLRTVPPLAWVPIALAALRQNEPAALFVIFITAVWPILLNTAVGVKQIPQDYRNVSRVLQLSKQKYFFKILIPSALPYIFTGLRISIGLAWLAIIAAEIIMSGIVGIGFFIWNSYTNDKVGEVILALVYIGAVGLILDRAVAWLQNVILPEEQK

Source

organism	Pseudanabaena sp. Chao 1811
ncbi_taxonomy_id	2963092
host_organism	Escherichia coli

Entity 2 polymer

description	CmpC
formula_weight	76933.0 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	C

MQDFVLVDQVERTFPLGGGKEYIALKGIDLQIRKGEFISLIGHSGCGKSTLLNMIAGLDLPSGGVVMLEDERVTRPGPDRMVVFQNYSLLPWLSVRENVALAVDEVLSHLPKGERHDLVERYVNMVGLAHAIDKPPTQLSGGMRQRVAIARALAVRPKLLLLDQPFGALDALTRGNLQEKLMQICNEDQITAVMVTHDVDEAVLLSDRIVMLTNGPASKIGNILEVDIPRPRQRMEAVNHPSYYSLRSEIIYFLNQQKRVKKLNARKVTTVAKHGLEKVNLEIGFVPLTACAPIAVAKEKGFFEKHGLDEVSLVRETSWRGIVDGISGGYLDAAQMPSGLPMWLTLGGAGACKPIVTALTMTRNGNAITLDKHFYDRGIHTLADFKAMLQSTRDRQHRMGVVHPSSMHNLLLRYWLAAGGIDPDLDVELKTIPPAQMVVDLKAGSIDGFCVGEPWNFRAAMENIGFTVATDLEIWNGHPGKVLGVREDWANAYPNTHIALVKALLEACMYCADPNNAEEIRQILSRRAYVSTNVNYIHLGNPDDVTCALDTPMREPAHHQFYGAGVNRPSRSEHLWHITQMARWGDVPFPRNWVEVLEKTCRVGVFSTAARELGLTDITYSRNAIQLFDDIPFNAEDPIGYLNHLDIKRNITMAEIAMESVRMVASSGDYKDHDGDYKDHDIDYKDDDDK

Source

organism	Pseudanabaena sp. Chao 1811
ncbi_taxonomy_id	2963092
host_organism	Escherichia coli

Entity 3 polymer

description	CmpD
formula_weight	31281.1 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	D

MTQTILEHTNQIAQIPPQESFLRIENVSKIYPTPKGEYVVLKDVNLHIQEGEFICLIGHSGCGKSTLLNMVGGFSKPSIGEVTVNGKLITKPGPDRMVVFQGYALLPWLTVYENVMLAVDSVNPNLSKSEKNDIVRHHLAMVGLSEAAEKKPTQISGGMKQRVSIARALAIRPEVLILDQPFGALDAITKEELQEELLQIWTEHRCTVLMITHDIDEALFLADRLVMMTNGPAANIGEIMRIPFPRPRDRAQIMEDPLYYDLRNTALDFLYNRFAHDE

Source

organism	Pseudanabaena sp. Chao 1811
ncbi_taxonomy_id	2963092
host_organism	Escherichia coli

Entity 4 non-polymer

description	ADENOSINE-5'-TRIPHOSPHATE
formula_weight	507.2 kDa
src_method	syn
entity_count	2

Entity 5 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	2

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.15 Å
resolution_method	—
num_particles	528220

fit	/app/data/saxs_computation/ua/9ua1/9ua1.fit
pddf	/app/data/pr_computation/ua/9ua1/9ua1.pr.out
profile	/app/data/saxs_computation/ua/9ua1/9ua1.dat

Cryo-EM structure of cyanobacterial bicarbonate transporter CmpBCD in complex with ATP

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 non-polymer

Entity 5 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)