Cryo-EM structure of HA11-bound alpha-synuclein fibril polymorph 6A6B

Method: ELECTRON MICROSCOPY D_max: 85.6 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9ufx
deposition_date	2025-04-10
title	Cryo-EM structure of HA11-bound alpha-synuclein fibril polymorph 6A6B
keywords	amyloid fibril, complex, PROTEIN FIBRIL; PROTEIN FIBRIL
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	27.60 Å
rg_electron	27.20 Å
i0	203993000.00
molecular_weight	71387.0 kDa
excluded_volume	64976 Å³
envelope_volume	109500 Å³
shell_volume	33636 Å³
envelope_diameter	90.0 Å
shell_rg	34.72 Å
envelope_rg	27.04 Å
shape_rg	27.18 Å
total_rg	27.71 Å
total_atoms	5016
n_residues	684
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	85.6 Å
rg_real	27.48 Å
rg_real_error	0.46 Å
i0_real	2.0400e+08
i0_real_error	2.3810e+06
rg_reciprocal	27.52 Å
i0_reciprocal	204000000.0000
total_estimate	0.9099
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	30.0 Å
skewness	0.156
kurtosis	-0.632
angular_range	— – 0.2850 Å⁻¹
current_alpha	0.0000
highest_alpha	21270000.0000
n_real_points	58
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.945; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.989

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.60 Å
resolution_method	—
num_particles	49647

description	Alpha-synuclein
formula_weight	14476.1 kDa
src_method	man
entity_count	12

type	polypeptide(L)
strand_id	A,D,E,F,B,C,G,J,K,L,H,I
atom_count	5016

Cryo-EM structure of HA11-bound alpha-synuclein fibril polymorph 6A6B

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

description	2',4',5',7'-tetrakis(iodanyl)-3',6'-bis(oxidanyl)spiro[2-benzofuran-3,9'-xanthene]-1-one
formula_weight	835.9 kDa
src_method	syn
entity_count	12

fit	/app/data/saxs_computation/uf/9ufx/9ufx.fit
pddf	/app/data/pr_computation/uf/9ufx/9ufx.pr.out
profile	/app/data/saxs_computation/uf/9ufx/9ufx.dat