Cryo-EM structure of HA21-bound alpha-synuclein fibril polymorph 6A6B

Method: ELECTRON MICROSCOPY D_max: 98.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9ufy
deposition_date	2025-04-10
title	Cryo-EM structure of HA21-bound alpha-synuclein fibril polymorph 6A6B
keywords	amyloid fibril, complex, PROTEIN FIBRIL; PROTEIN FIBRIL
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	28.58 Å
rg_electron	28.59 Å
i0	81794900.00
molecular_weight	74790.0 kDa
excluded_volume	95507 Å³
envelope_volume	119740 Å³
shell_volume	35042 Å³
envelope_diameter	102.7 Å
shell_rg	35.82 Å
envelope_rg	28.96 Å
shape_rg	28.58 Å
total_rg	29.35 Å
total_atoms	5272
n_residues	720
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	98.5 Å
rg_real	28.57 Å
rg_real_error	0.92 Å
i0_real	8.1790e+07
i0_real_error	1.2230e+06
rg_reciprocal	28.58 Å
i0_reciprocal	81800000.0000
total_estimate	0.8709
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	30.4 Å
skewness	0.320
kurtosis	-0.425
angular_range	— – 0.2750 Å⁻¹
current_alpha	0.0000
highest_alpha	17370000.0000
n_real_points	56
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.810; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.931; Smooth: 0.958

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.20 Å
resolution_method	—
num_particles	88070

description	Alpha-synuclein
formula_weight	14476.1 kDa
src_method	man
entity_count	12

type	polypeptide(L)
strand_id	A,D,E,F,B,C,G,J,K,L,H,I
atom_count	5272

Cryo-EM structure of HA21-bound alpha-synuclein fibril polymorph 6A6B

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

description	Gossypol
formula_weight	518.6 kDa
src_method	syn
entity_count	8

fit	/app/data/saxs_computation/uf/9ufy/9ufy.fit
pddf	/app/data/pr_computation/uf/9ufy/9ufy.pr.out
profile	/app/data/saxs_computation/uf/9ufy/9ufy.dat