9utz
The structure of the E147K/Q174E/D197K triple mutant of PES-H1
SAXS Profile
P(r) Distribution
1. Structure Basics
| entry_id | 9utz |
| deposition_date | 2025-05-05 |
| last_revision | 2026-05-13 |
| title | The structure of the E147K/Q174E/D197K triple mutant of PES-H1 |
| keywords | PET hydrolase, enzyme engineering, salt tolerance, HYDROLASE; HYDROLASE |
| method | X-RAY DIFFRACTION |
Download Data
2. SAXS Parameters (CRYSOL)
| rg_guinier | 24.87 Å |
| rg_electron | 23.82 Å |
| i0 | 54158400.00 |
| molecular_weight | 55766.0 kDa |
| excluded_volume | 69075 ų |
| envelope_volume | 78271 ų |
| shell_volume | 27088 ų |
| envelope_diameter | 81.6 Å |
| shell_rg | 31.29 Å |
| envelope_rg | 23.88 Å |
| shape_rg | 23.81 Å |
| total_rg | 24.64 Å |
| total_atoms | 3931 |
| n_residues | 517 |
| n_harmonics | 20 |
| q_range | — – 0.5000 Å−1 |
| n_points | 101 |
| shell_type | directional |
| solvent_density | 0.3340 e/ų |
| contrast_shell | 0.0300 e/ų |
| crysol_version | 4.1.3 |
3. P(r) Analysis (GNOM)
| dmax | 80.8 Å |
| rg_real | 24.82 Å |
| rg_real_error | 0.47 Å |
| i0_real | 5.4160e+07 |
| i0_real_error | 7.9560e+05 |
| rg_reciprocal | 24.84 Å |
| i0_reciprocal | 54160000.0000 |
| total_estimate | 0.8952 |
| solution_quality | GOOD a GOOD solution |
| n_peaks | 1 |
| r_peak_primary | 79.5 Å |
| skewness | 0.272 |
| kurtosis | -0.546 |
| angular_range | — – 0.3200 Å−1 |
| current_alpha | 0.0000 |
| highest_alpha | 11060000.0000 |
| n_real_points | 64 |
| gnom_version | 4.1.3 |
| quality_criteria | AN1: 0.000; Oscil: 0.889; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.984; Smooth: 0.981 |