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9w26

Cryo-EM structure of TasH-tigRNA (5G)-dsDNA complex

Method: ELECTRON MICROSCOPY D_max: 89.3 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9w26
deposition_date	2025-07-26
title	Cryo-EM structure of TasH-tigRNA (5G)-dsDNA complex
keywords	protein complex, ANTIVIRAL PROTEIN; ANTIVIRAL PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	28.37 Å
rg_electron	28.02 Å
i0	212921000.00
molecular_weight	93161.0 kDa
excluded_volume	106730 Å³
envelope_volume	136330 Å³
shell_volume	39841 Å³
envelope_diameter	92.3 Å
shell_rg	36.19 Å
envelope_rg	27.95 Å
shape_rg	28.04 Å
total_rg	28.56 Å
total_atoms	6412
n_residues	645
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	89.3 Å
rg_real	28.28 Å
rg_real_error	0.53 Å
i0_real	2.1290e+08
i0_real_error	3.2860e+06
rg_reciprocal	28.31 Å
i0_reciprocal	212900000.0000
total_estimate	0.7168
solution_quality	REASONABLE a REASONABLE solution
n_peaks	1
r_peak_primary	87.7 Å
skewness	0.309
kurtosis	-0.336
angular_range	— – 0.2800 Å⁻¹
current_alpha	0.0000
highest_alpha	35670000.0000
n_real_points	57
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.896; Stabil: 1.000; Sysdev: 0.242; Positv: 1.000; Valcen: 0.998; Smooth: 0.901

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	TasH
formula_weight	40031.2 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	6412

MNKQVLKEQASHCEITGAPLAGLPELVDVDRITERFQGGTYTPDNTRVLTPRAHMERHGILRERDQWLEELKAMMDDRAQTMKVVMKMNNQLLAYQRQTDHARQSTEQFLQDTLDASNKRLAQIDREVTKHIKHAKDPLAQAAMGVPGVGPITVAGLQTYVDLEKAKSASALWAYIGIDKPSHDRYTKGEAGGGNKTLRTMVWNMANSMIKNRKCPYRTVYEQTKERLAVSEKVTKSRNTQGQLIECAWKDTKPSHRHGAALRAVMKHFLADYWFVGRELAGLDTRPLYVQEKLGHTGIVQPQERGWEWGGSWSHPQFEKGGGSGGGSGGSAWSHPQFEKNLYFQSGSHHHHHH

Source

organism	Salicola phage CGphi29
ncbi_taxonomy_id	754067
host_organism	Escherichia coli

Entity 2 polymer

description	RNA (36-MER)
formula_weight	11964.2 kDa
src_method	man
entity_count	1

Polymer

type	polyribonucleotide
strand_id	C

AGUCGUUCCGUUAAAGACAACCACGGAGACGAAGCGA

Source

organism	Salicola phage CGphi29
ncbi_taxonomy_id	754067
host_organism	Escherichia coli

Entity 3 polymer

description	DNA (38-MER)
formula_weight	11606.5 kDa
src_method	man
entity_count	2

Polymer

type	polydeoxyribonucleotide
strand_id	D,E

CCCTAAGGCAATTCCGTTACGTCTCCGTGTTACAGGAC

Source

organism	Salicola phage CGphi29
ncbi_taxonomy_id	754067
host_organism	Escherichia coli

Entity 4 polymer

description	DNA (38-MER)
formula_weight	11784.6 kDa
src_method	man
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	F

GTCCTGTAACACGGAGACGTAACGGAATTGCCTTAGGG

Source

organism	Salicola phage CGphi29
ncbi_taxonomy_id	754067
host_organism	Escherichia coli

Entity 5 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	1

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.62 Å
resolution_method	—
num_particles	314173

fit	/app/data/saxs_computation/w2/9w26/9w26.fit
pddf	/app/data/pr_computation/w2/9w26/9w26.pr.out
profile	/app/data/saxs_computation/w2/9w26/9w26.dat

Cryo-EM structure of TasH-tigRNA (5G)-dsDNA complex

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 polymer

Polymer

Source

Entity 5 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)