SAXSdb — 9waw

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1. Structure Basics

entry_id	9waw
deposition_date	2025-08-12
title	SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class E
keywords	Complex, cas, SuperFi cas9, IMMUNE SYSTEM; IMMUNE SYSTEM
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	38.99 Å
rg_electron	39.03 Å
i0	545237000.00
molecular_weight	162070.0 kDa
excluded_volume	190880 Å³
envelope_volume	287120 Å³
shell_volume	60159 Å³
envelope_diameter	141.0 Å
shell_rg	45.77 Å
envelope_rg	38.53 Å
shape_rg	39.04 Å
total_rg	39.35 Å
total_atoms	11240
n_residues	1160
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	132.7 Å
rg_real	38.89 Å
rg_real_error	1.01 Å
i0_real	5.4520e+08
i0_real_error	9.0190e+06
rg_reciprocal	38.96 Å
i0_reciprocal	545300000.0000
total_estimate	0.6637
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	45.3 Å
skewness	0.255
kurtosis	-0.422
angular_range	— – 0.2050 Å⁻¹
current_alpha	0.0000
highest_alpha	54620000.0000
n_real_points	42
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.846; Stabil: 1.000; Sysdev: 0.035; Positv: 1.000; Valcen: 1.000; Smooth: 0.980

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	CRISPR-associated endonuclease Cas9/Csn1
formula_weight	158502.4 kDa
ec_number	3.1.-.-
mutation	Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, K1031D
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	11240

MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVDGDDKVDDDDKMIAKSEDEIGDATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD

Source

organism	Streptococcus pyogenes
ncbi_taxonomy_id	1314
host_organism	Escherichia coli

Entity 2 polymer

description	DNA (50-MER)
formula_weight	15438.9 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	B

AGCTATGAGTGGCCCCTGTGGAGAGAAGCCTGTCCTGGAAGCCAGCCCCA

Entity 3 polymer

description	DNA (50-MER)
formula_weight	15371.8 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	C

TGGGGCTGGCTTCCAGGACAGGCTTCTCTCCACAGGGGCCACTCATAGCT

Entity 4 polymer

description	RNA (102-MER)
formula_weight	32815.5 kDa
src_method	syn
entity_count	1

Polymer

type	polyribonucleotide
strand_id	D

CAGGACAGGCUUCUCUCCACAGGUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUU

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.950 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	3.95 Å
resolution_method	—
num_particles	52037

fit	/app/data/saxs_computation/wa/9waw/9waw.fit
pddf	/app/data/pr_computation/wa/9waw/9waw.pr.out
profile	/app/data/saxs_computation/wa/9waw/9waw.dat

SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class E

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Entity 4 polymer

Polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)