SAXSdb — 9wey

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1. Structure Basics

entry_id	9wey
deposition_date	2025-08-20
title	Structure of HCMV UL33 in complex with human Gs protein
keywords	HCMV, viral protein, viral-host interaction, GPCR, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	35.87 Å
rg_electron	35.93 Å
i0	204120000.00
molecular_weight	115010.0 kDa
excluded_volume	143900 Å³
envelope_volume	186210 Å³
shell_volume	44502 Å³
envelope_diameter	129.8 Å
shell_rg	40.65 Å
envelope_rg	36.35 Å
shape_rg	35.90 Å
total_rg	36.33 Å
total_atoms	8084
n_residues	1046
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	121.9 Å
rg_real	36.13 Å
rg_real_error	1.15 Å
i0_real	2.0410e+08
i0_real_error	3.4820e+06
rg_reciprocal	35.97 Å
i0_reciprocal	204100000.0000
total_estimate	0.8402
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	36.0 Å
skewness	0.559
kurtosis	-0.162
angular_range	— – 0.2200 Å⁻¹
current_alpha	0.0000
highest_alpha	49930000.0000
n_real_points	45
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.792; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.889; Smooth: 0.654

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
formula_weight	7729.9 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	C
atom_count	8084

ASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFREKKFFCAIL

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 2 polymer

description	Nanobody35
formula_weight	15969.6 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	N

QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQGTQVTVSSHHHHHHEPEAHHHHHH

Source

organism	Lama glama
ncbi_taxonomy_id	9844
host_organism	Escherichia coli

Entity 3 polymer

description	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
formula_weight	45568.2 kDa
ec_number	3.6.5.-
mutation	S54N, G226A, E268A, N271K, K274D, R280K, T284D, I285T, A366S
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A

GCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENIRRVFNDCRDIIQRMHLRQYELL

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 4 polymer

description	;Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, HiBit fusion ;
formula_weight	40039.6 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

GPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSGGGGSSGVSGWRLFKKIS

Source

organism	Homo sapiens
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 5 polymer

description	;G-protein coupled receptor homolog UL33,G-protein coupled receptor homolog UL33,G-protein coupled receptor homolog UL33, LgBit fusion ;
formula_weight	68188.7 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	R

MDTIIHNSTRNNTPPHINDTCNMTGPLFAIRTTEAVLNTFIIFVGGPLNAIVLITQLLTNRVLGYSTPTIYMTNLYSTNFLTLTVLPFIVLSNQWLLPAGVASCKFLSVIYYSSCTVGFATVALIAADRYRVLHKRTYARQSYRSTYMILLLTWLAGLIFSVPAAVYTTVVMHHDANDTNNTNGHATCVLYFVAEEVHTVLLSWKVLLTMVWGAAPVIMMTWFYAFFYSTVQRTSQKQRSRTLTFVSVLLISFVALQTPYVSLMIFNSYATTAWPMQCEHLTLRRTIGTLARVVPHLHCLINPILYALLGHDFLQRMRQCFRGQLLDRRAFLRSQQNQRATAETNLAAGNNSQSVATSLDTNSKNYNQHAKRSVSFNFPSGTWKGGQKTASNDTSTKIPHRLSQSHHNLSGVAAAEDQVDPRLIDGKSRGSSGGGGSGGGGSSGVFTLEDFVGDWEQTAAYNLDQVLEQGGVSSLLQNLAVSVTPIQRIVRSGENALKIDIHVIIPYEGLSADQMAQIEEVFKVVYPVDDHHFKVILPYGTLVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLITPDGSMLFRVTINSGGGGSHHHHHHHH

Source

organism	Human betaherpesvirus 5
ncbi_taxonomy_id	10359
host_organism	Human betaherpesvirus 5

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.300 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	3.30 Å
resolution_method	—
num_particles	54507

fit	/app/data/saxs_computation/we/9wey/9wey.fit
pddf	/app/data/pr_computation/we/9wey/9wey.pr.out
profile	/app/data/saxs_computation/we/9wey/9wey.dat

Structure of HCMV UL33 in complex with human Gs protein

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 polymer

Polymer

Source

Entity 5 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)