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9wgz

Tryptophan hydroxylase mutant - Y235S

Method: X-RAY DIFFRACTION D_max: 62.2 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9wgz
deposition_date	2025-08-25
last_revision	2026-02-11
title	Tryptophan hydroxylase mutant - Y235S
keywords	mutant, OXIDOREDUCTASE; OXIDOREDUCTASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	19.89 Å
rg_electron	18.84 Å
i0	20698200.00
molecular_weight	35482.0 kDa
excluded_volume	44689 Å³
envelope_volume	50931 Å³
shell_volume	21904 Å³
envelope_diameter	64.2 Å
shell_rg	25.95 Å
envelope_rg	19.08 Å
shape_rg	18.82 Å
total_rg	19.86 Å
total_atoms	2502
n_residues	309
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	62.2 Å
rg_real	19.72 Å
rg_real_error	0.31 Å
i0_real	2.0700e+07
i0_real_error	2.6560e+05
rg_reciprocal	19.75 Å
i0_reciprocal	20700000.0000
total_estimate	0.8946
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	26.3 Å
skewness	0.090
kurtosis	-0.475
angular_range	— – 0.4000 Å⁻¹
current_alpha	0.0000
highest_alpha	8203000.0000
n_real_points	72
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.883; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.991; Smooth: 0.985

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	Tryptophan 5-hydroxylase 1
formula_weight	36992.2 kDa
ec_number	1.14.16.4
mutation	Y235S
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	2836

MDGMETVPWFPKKISDLDHCANRVLMYGSELDADHPGFKDNVYRKRRKYFADLAMNYKHGDPIPKVEFTEEEIKTWGTVFQELNKLYPTHACREYLKNLPLLSKYCGYREDNIPQLEDVSNFLKERTGFSIRPVAGSLSPRDFLSGLAFRVFHCTQYVRHSSDPFYTPEPDTCHELLGHVPLLAEPSFAQFSQEIGLASLGASEEAVQKLATCYFFTVEFGLCKQDGQLRVFGAGLLSSISELKHALSGHAKVKPFDPKITCKQECLITTFQDVYFVSESFEDAKEKMREFTKTIKRPFGVKYNPYTRSIQILKDTKSITSAM

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli BL21(DE3)

Entity 2 non-polymer

description	FE (III) ION
formula_weight	55.8 kDa
src_method	syn
entity_count	1

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	334

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6. Citations (1)

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length_a	123.340 Å
length_b	123.340 Å
length_c	46.560 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 43 21 2
int_tables_number	96
crystal_system	—

resolution	1.857 Å
resolution_low	43.560 Å
r_factor	0.1817
r_work	0.1802
r_free	0.2085

fit	/app/data/saxs_computation/wg/9wgz/9wgz.fit
pddf	/app/data/pr_computation/wg/9wgz/9wgz.pr.out
profile	/app/data/saxs_computation/wg/9wgz/9wgz.dat

Tryptophan hydroxylase mutant - Y235S

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction