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9ylu

cryoEM structure of CbCash filament with cA4 and tRNA Ile CAU

Method: ELECTRON MICROSCOPY D_max: 148.5 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9ylu
deposition_date	2025-10-09
title	cryoEM structure of CbCash filament with cA4 and tRNA Ile CAU
keywords	tRNA endonuclease, cA4 activation, CRISPR associated Schlafen, Csx15, RNA BINDING PROTEIN; RNA BINDING PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	47.16 Å
rg_electron	46.26 Å
i0	1234310000.00
molecular_weight	280970.0 kDa
excluded_volume	347450 Å³
envelope_volume	487240 Å³
shell_volume	83787 Å³
envelope_diameter	144.9 Å
shell_rg	54.28 Å
envelope_rg	45.23 Å
shape_rg	46.26 Å
total_rg	46.55 Å
total_atoms	19677
n_residues	2401
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	148.5 Å
rg_real	46.89 Å
rg_real_error	0.78 Å
i0_real	1.2340e+09
i0_real_error	2.1360e+07
rg_reciprocal	47.16 Å
i0_reciprocal	1235000000.0000
total_estimate	0.9049
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	1
r_peak_primary	58.5 Å
skewness	0.064
kurtosis	-0.733
angular_range	— – 0.1650 Å⁻¹
current_alpha	0.0001
highest_alpha	137000000.0000
n_real_points	34
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.941; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.987; Smooth: 0.949

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	CbCash
formula_weight	33530.6 kDa
src_method	man
entity_count	8

Polymer

type	polypeptide(L)
strand_id	A,B,C,D,E,F,G,H
atom_count	19677

MDKLWRLLGNYVDSGRETRKVDLKREIEMKAGAGKFAKDVAAIANTIGGTGYLIIGVIDLKERKSNSPDDYVTGWNVTDLDGFSRWVNQSLSNYCNPIPRIEIEEMTHPVCGKKIGVIIIPRSLDCPHEIIRDGDGITKGMYVRRGADTFTASREELRAMMGGTDVRIVLNFLQPLTQLNREQIGQYLNARVYEVLEPRSLPIRLDENLSFVEQAKILLNETGLTPEEWRTLPLIVNLPGFSPLSSILLAEMQGRMSRLPHIIRMRPTPADRTIFEVAEVIALQDIRDKALEWSSGI

Source

organism	Chloroflexi bacterium 13_1_20CM_2_59_7
ncbi_taxonomy_id	1805056
host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

description	tRNA Ile CAU
formula_weight	24445.5 kDa
src_method	syn
entity_count	2

Polymer

type	polyribonucleotide
strand_id	I,J

GGCCCCUUAGCUCAGUGGUUAGAGCAGGCGACUCAUAAUCGCUUGGUCGCUGGUUCAAGUCCAGCAGGGGCCACCA

Entity 3 polymer

description	cyclic-tetra-adenylate
formula_weight	1271.9 kDa
src_method	syn
entity_count	4

Polymer

type	polyribonucleotide
strand_id	K,L,M,N

AAAA

Entity 4 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	2

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6. Citations (1)

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resolution	2.65 Å
resolution_method	—
num_particles	305770

fit	/app/data/saxs_computation/yl/9ylu/9ylu.fit
pddf	/app/data/pr_computation/yl/9ylu/9ylu.pr.out
profile	/app/data/saxs_computation/yl/9ylu/9ylu.dat

cryoEM structure of CbCash filament with cA4 and tRNA Ile CAU

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

EM 3D

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Entity 4 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)