9zc8

5-methyl-cytidine RNA origami 6-helix bundle type-1 dimer

Method: ELECTRON MICROSCOPY Dmax: 225.1 Å Quality: GOOD

1. Protein Identity and Related Structures Protein Identity & Related Structures

No usable UniProt protein identity is available for this entry.

七张关系表仍保留该条目的 assembly 与组成信息,但缺少统一蛋白身份时,不能可靠建立跨 PDB 的同蛋白Chain接。

Assembly Composition of the Current Entry

Assembly Oligomeric State 实体与Construct证据 Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Nucleic acid only No protein 蛋白 0 / DNA 0 / RNA 2 / 其他Polymer 0 PDB declaration: dimeric Entity 1:5-methyl-cytidine RNA origami 6-helix bundle × 2 缺少 UniProt 身份时不显示参考序列区间 Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule ELECTRON MICROSCOPY cryo-EM buffer:pH 8;50mM HEPES pH 8.0, 50mM KCL, 5mM MgCl2cryo-EM vitrification conditions:Cryogen ETHANE Resolution 12.10 Å

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 9zc8

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 9zc8
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2. Structure Basics 2. Structure Basics

Entry ID entry_id9zc8
Deposition date deposition_date2025-11-22
Structure title title5-methyl-cytidine RNA origami 6-helix bundle type-1 dimer
Keywords keywordsRNA, origami, 5mC, 5-methyl-cytidine, modified base, homodimer, dimer; RNA
Experimental Method methodELECTRON MICROSCOPY

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier80.31
Radius of gyration Rg (electron density) rg_electron80.72
Forward intensity I(0) i09296000000.00
Molecular weight molecular_weight470510.0 kDa
Excluded volume excluded_volume444010 ų
Envelope volume envelope_volume1001800 ų
Hydration-shell volume shell_volume109010 ų
Envelope diameter envelope_diameter274.8
Shell Rg shell_rg72.36
Envelope Rg envelope_rg76.26
Shape Rg shape_rg80.68
Total Rg total_rg80.72
Total atoms total_atoms31034
Residues n_residues1082
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 −1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax225.1
Rg (real space) rg_real79.10
Rg uncertainty (real space) rg_real_error0.86
I(0) (real space) i0_real9.1130e+09
I(0) uncertainty (real space) i0_real_error1.5440e+08
Rg (reciprocal space) rg_reciprocal78.92
I(0) (reciprocal space) i0_reciprocal9260000000.0000
Solution quality estimate total_estimate0.8567
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary83.0
Skewness Skewness skewness0.289
Kurtosis Kurtosis kurtosis-0.668
Angular range angular_range— – 0.0950 −1
Current regularization parameter α current_alpha0.7525
Highest regularization parameter α highest_alpha179000000.0000
Real-space data points n_real_points20
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 1.000; Stabil: 0.991; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.002

5. Crystallography and Experiment 5. Crystallography & Experiment

6. Entities and Polymers Entities & Polymers (1)

8. Citations (1)

9. Files and Curves (10)