SAXSdb — 9zs2

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1. Structure Basics

entry_id	9zs2
deposition_date	2025-12-22
title	S. marcescens Cas10-Csm unbound to target RNA
keywords	CRISPR, crRNA, Cas10, type III, RNA BINDING PROTEIN; RNA BINDING PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	58.33 Å
rg_electron	59.48 Å
i0	1059700000.00
molecular_weight	263530.0 kDa
excluded_volume	326300 Å³
envelope_volume	482400 Å³
shell_volume	70416 Å³
envelope_diameter	215.3 Å
shell_rg	56.08 Å
envelope_rg	59.61 Å
shape_rg	59.49 Å
total_rg	59.37 Å
total_atoms	18542
n_residues	2338
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	192.7 Å
rg_real	59.08 Å
rg_real_error	2.22 Å
i0_real	1.0600e+09
i0_real_error	2.3950e+07
rg_reciprocal	57.65 Å
i0_reciprocal	1057000000.0000
total_estimate	0.7386
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	42.8 Å
skewness	0.531
kurtosis	-0.529
angular_range	— – 0.1350 Å⁻¹
current_alpha	0.0000
highest_alpha	118700000.0000
n_real_points	28
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.653; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.584; Smooth: 0.057

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)
formula_weight	90390.7 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	18542

MNWLAASCHVAAFALLHNLKPLAQRAGIQDFPSPALANKLFAPLPESLSARADLHENTLTLLLDFAARLARGLPETPVSRGETGLIPLTHLLADDNHSAPAERCYSPLAPLGVDSLMPVTAALPTGERQAAYRQVYQALIEGLEAIPAAHRLQPSLWLDHLDSLWMTTCHALPDGDGASGISLYDQGKTTAALAVALWQHHARQATPEKEISLTPGDDARLLLIQADVFGIQELIFAQGNQTQKMAAKLLRGRSFQVSLLAETAALRVLETFDLPPVCQLINAAGKSLIVAPNLPDAAERLAGLRQRLDEWFLTHTYAQTGIGLCSTVAGSAEFLGEQAYGRLQNRLAQAMEQQKYQRFSLCAGDAPPPVFEGYLDQIAQGAGGEPCPMNGLHPVETTLNGLGCSRLAADQITLGHWINHGHWISHGERYARLLILRDSTATFSDDRCLHLTLFGYQVVAVSLKEDSGEFGELACNGSLRRCWDVSLPPEGTSLFQGYARRFINGWMPLAQGEYQPECYPGIEEALANGDIKTFDHLSCEDLYQDADQSLRGTCALGVLKGDIDNLGHLFRSGLPQPGFAKTIGLSRQIHLFFTLWLPHLCRKDPRFANTYTVFAGGDDFFLIGPWRSQQTLALTMAQDFARYSGHNPALHFSLGLVQAKPGYPVRALAAQAEAALKQAKQHPGKNAICLYNEVMGWPEYDALLACSEELARWREHDGYPLSSGLLYRLLALSEQSAEESEKPQAALWRSRLAYFLRRNLVDNVKVPTKENPAAFRHQLHLELFEKLEQHLKRHRQRYRVALQRHLYHYRTVPRGE

Source

organism	Serratia
ncbi_taxonomy_id	613
host_organism	Escherichia coli

Entity 2 polymer

description	CRISPR system Cms endoribonuclease Csm3
formula_weight	27185.9 kDa
mutation	D43A
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	E,F,G,H

MQLNNIQTLRATLVCETGLHIGGGDTALQIGGIASAVVRHPLTQQPYIPGSSLKGKLRSLLEWRAGVVGDTEGKVLSHQVYQQLIDDKKQAQALQALKILQLFGVSGGDKLSAEQAQQIGPTRLSFWDCEFDEHWLAQQGGRVQTEEKAENCIDRISGVALHPRFIERVPAGSRFDFRLTVRQLDGDSPDLLDTLLAGLKMLELDGLGGSISRGYGKVRFEALTLDGKDLQPRFEQLQPFKHTTQGAG

Source

organism	Serratia
ncbi_taxonomy_id	613
host_organism	Escherichia coli

Entity 3 polymer

description	CRISPR system Cms protein Csm4
formula_weight	35770.6 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	I

MPSPVAPAAAWQWLRLRLLPDSAFATALRGDTLFGQLCWYLRESLGEAGLNALLAGYHDQRPFAVISDPMLADHLPRPHLPEHRLGFAAADARARKQRKQQCWLPLAFAHQPLAEWGAHLTAAPEHHQHRSDVQMHNSINRQTLTTGGDDAFAPFGSEQHWFDVDTAWDLYLLHDKRLSAAQLTDALRAIGLLGYGKDASIGKGRFHLTPQPMPELPTPDGNPLRLTLAPCAPQQGHWHSADSFFSPLVRVGRHGAALVVQGHPFKNPLLLADSAAVLAPVTPDNRPFVGQGLGGNGRLSTALPQTVHQGYAPVIPVRFHHKAQPQ

Source

organism	Serratia
ncbi_taxonomy_id	613
host_organism	Escherichia coli

Entity 4 polymer

description	CRISPR system Cms protein Csm5
formula_weight	63148.7 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	J

MTRDQTPRRHTACDSDNMQYFTLTCLSPVHVATGDSLNPGEYLIDENALYELGQGGLSPALTATQRSELLTILESNDPALPLTVQRFLAREAGKLKYAARRMCPLLPGISRYYQSRLGQVMQNDTKNKKQMINQLELMRHVGAALGAPYIPGSTLKGAIRTALVSALNQGQPLQAERRETDKLSSKVAQDAERQLLGFDTRRDSPRYRIEHDPFHWLQVGDAVSPAEHPPMLDYWLVRRQPFKRTEKQDNKADNMELSPVECLKPRQSPLHCQITVKTPPTALAIKNPRLKQWLGKVSQLAQQVNRITLPQCHHELAWLAEKHIGTDDVYAPGQNWVAQMQQLLRQLDDPLQRGEALLLRVGKYGGAISKTVAGWRHIARLGRQGTRTTYHPDVTTCTLALPQADALTQALPFGWVLLHQPDQPEVTEFVASHHDWCQQQQQRLDAHQQQQHTHRQQRQQLAQAREEEAQRLADKARQSKARQSIMSLAEQLASEQTFQHKNPNGPLRGQLATCVGCVATEGSAEEKAELCTLFDDILNYWGIKPGKDKKLTALRNKLL

Source

organism	Serratia
ncbi_taxonomy_id	613
host_organism	Escherichia coli

Entity 5 polymer

description	RNA (30-MER)
formula_weight	13361.9 kDa
src_method	man
entity_count	1

Polymer

type	polyribonucleotide
strand_id	K

AUUAAGACCAUUUACAGAACCUGUUCCACUUAAUGAAGUUGC

Source

organism	Serratia
ncbi_taxonomy_id	613
host_organism	Escherichia coli

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	4.40 Å
resolution_method	—
num_particles	42471

fit	/app/data/saxs_computation/zs/9zs2/9zs2.fit
pddf	/app/data/pr_computation/zs/9zs2/9zs2.pr.out
profile	/app/data/saxs_computation/zs/9zs2/9zs2.dat

S. marcescens Cas10-Csm unbound to target RNA

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 polymer

Polymer

Source

Entity 5 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)