Current Protein Identity:A6ZZ25
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 3JAP Structure of a partial yeast 48S preinitiation complex in closed conformation Deposited 2015-06-18 | Assembly 1 Protein–RNA Heteromer;Protein × 44 PDB declaration: 47-meric(47) Consistent with all polymers |
Chain s
45–96(52 aa)
|
Not recorded | MG MAGNESIUM ION × 81 ZN ZINC ION × 4 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
20 mM MES-KOH, 40 mM potassium acetate, 10 mM ammonium acetate, 8 mM magnesium acetate, 2 mM DTT;pH 6.5;20 mM MES-KOH, 40 mM potassium acetate, 10 mM ammonium acetate, 8 mM magnesium acetate, 2 mM DTT
cryo-EM vitrification conditions
Blot for 2.5 to 3 seconds before plunging;120 K;Cryogen ETHANE;Blot for 2.5 to 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK I).
|
Resolution 4.90 Å |
| 6FYX Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C1) Deposited 2018-03-12 | Assembly 1 Protein–RNA Heteromer;Protein × 44 PDB declaration: 47-meric(47) Consistent with all polymers |
Chain r
1–274(274 aa)
|
Not recorded | MG MAGNESIUM ION × 117 ZN ZINC ION × 5 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6FYY Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C2) Deposited 2018-03-12 | Assembly 1 Protein–RNA Heteromer;Protein × 44 PDB declaration: 47-meric(47) Consistent with all polymers |
Chain r
1–274(274 aa)
|
Not recorded | MG MAGNESIUM ION × 117 ZN ZINC ION × 5 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.02 Å |
| 6GSN Structure of a partial yeast 48S preinitiation complex in closed conformation Deposited 2018-06-14 | Assembly 1 Protein–RNA Heteromer;Protein × 44 PDB declaration: 47-meric(47) Consistent with all polymers |
Chain r
48–96(49 aa)
|
Not recorded | MG MAGNESIUM ION × 81 ZN ZINC ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.75 Å |