Current Protein Identity：B7MIU7
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 5U9F 3.2 A cryo-EM ArfA-RF2 ribosome rescue complex (Structure II) Deposited 2016-12-16 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain P
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 360 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 5U9G 3.2 A cryo-EM ArfA-RF2 ribosome rescue complex (Structure I) Deposited 2016-12-16 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain P
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 348 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 5UZ4 The cryo-EM structure of YjeQ bound to the 30S subunit suggests a fidelity checkpoint function for this protein in ribosome assembly Deposited 2017-02-24 | Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 21-meric（21） Consistent with all polymers |
Chain P
1–82（82 aa）
|
Not recorded | ZN ZINC ION × 1 GGM 3'-O-(N-methylanthraniloyl)-beta:gamma-imidoguanosine-5'-triphosphate × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.80 Å |
| 5WF0 70S ribosome-EF-Tu H84A complex with GTP and near-cognate tRNA (Complex C2) Deposited 2017-07-11 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain p
1–82（82 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 MG MAGNESIUM ION × 1480 K POTASSIUM ION × 5 PHE PHENYLALANINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；5 second blot time,
no wait time
|
Resolution 3.60 Å |
| 5WFS 70S ribosome-EF-Tu H84A complex with GTP and near-cognate tRNA (Complex C4) Deposited 2017-07-12 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain p
1–82（82 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 MG MAGNESIUM ION × 1471 K POTASSIUM ION × 7 PHE PHENYLALANINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；5 second blot time,
no wait time
|
Resolution 3.00 Å |
| 6AWB Structure of 30S ribosomal subunit and RNA polymerase complex in non-rotated state Deposited 2017-09-05 | Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 27-meric（27） Consistent with all polymers |
Chain S
1–82（82 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7；20 mM Tris-HCl, pH 7.0, 100 mM NH4Cl, 10 mM MgCl2, 0.5 mM EDTA, 6 mM BME
cryo-EM vitrification conditions
Cryogen ETHANE；2.5 uL of 50 nM 30S and 150 nM RNAP was applied to the grid. After a 30 second incubation, the grid was blotted for 5 seconds at blotting power 8.
|
Resolution 6.70 Å |
| 6AWC Structure of 30S ribosomal subunit and RNA polymerase complex in rotated state Deposited 2017-09-05 | Assembly 1 Protein–RNA Heteromer；Protein × 26 PDB declaration: 27-meric（27） Consistent with all polymers |
Chain S
1–82（82 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7；20 mM Tris-HCl, pH 7.0, 100 mM NH4Cl, 10 mM MgCl2, 0.5 mM EDTA, 6 mM BME
cryo-EM vitrification conditions
Cryogen ETHANE；2.5 uL of 50 nM 30S and 150 nM RNAP was applied to the grid. After a 30 second incubation, the grid was blotted for 5 seconds at blotting power 8.
|
Resolution 7.90 Å |
| 6AWD Structure of 30S (S1 depleted) ribosomal subunit and RNA polymerase complex Deposited 2017-09-05 | Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 26-meric（26） Consistent with all polymers |
Chain S
1–82（82 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7；20 mM Tris-HCl, pH 7.0, 100 mM NH4Cl, 10 mM MgCl2, 0.5 mM EDTA, 6 mM BME
cryo-EM vitrification conditions
Cryogen ETHANE；2.5 uL of 50 nM 30S and 150 nM RNAP was applied to the grid. After a 30 second incubation, the grid was blotted for 5 seconds at blotting power 8.
|
Resolution 8.10 Å |
| 6DNC E.coli RF1 bound to E.coli 70S ribosome in response to UAU sense A-site codon Deposited 2018-06-06 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain CB
1–82（82 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7；20 mM Tris-HCl, 100 mM NH4Cl, 20.5 mM MgCl2, 0.5 mM EDTA, 6 mM beta-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6O7K 30S initiation complex Deposited 2019-03-08 | Assembly 1 Protein–RNA Heteromer；Protein × 22 PDB declaration: 25-meric（25） Consistent with all polymers |
Chain y
1–82（82 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 6O9J 70S Elongation Competent Ribosome Deposited 2019-03-14 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain p
1–82（82 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6O9K 70S initiation complex Deposited 2019-03-14 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain p
1–82（82 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 6OGF 70S termination complex with RF2 bound to the UGA codon. Partially rotated ribosome with RF2 bound (Structure III). Deposited 2019-04-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6OGG 70S termination complex with RF2 bound to the UGA codon. Rotated ribosome with RF2 bound (Structure IV). Deposited 2019-04-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 6OGI 70S termination complex with RF2 bound to the UAG codon. Rotated ribosome conformation (Structure V) Deposited 2019-04-02 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 6OM6 Structure of trans-translation inhibitor bound to E. coli 70S ribosome with P site tRNA Deposited 2019-04-18 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain u
1–82（82 aa）
|
Not recorded | KKL 4-amino-N-[5-(4-ethynylphenyl)-1,3,4-oxadiazol-2-yl]benzamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Blot time: 3.5 seconds
|
Resolution 3.10 Å |
| 6ORE Release complex 70S Deposited 2019-04-30 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain u
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 436 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.90 Å |
| 6ORL RF1 pre-accommodated 70S complex at 24 ms Deposited 2019-04-30 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain u
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 295 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.50 Å |
| 6OSK RF1 accommodated 70S complex at 60 ms Deposited 2019-05-01 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain u
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 307 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.60 Å |
| 6OSQ RF1 accommodated state bound Release complex 70S at long incubation time point Deposited 2019-05-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain u
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 436 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.50 Å |
| 6OST RF2 pre-accommodated state bound Release complex 70S at 24ms Deposited 2019-05-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain u
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 12 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.20 Å |
| 6OT3 RF2 accommodated state bound Release complex 70S at 24 ms Deposited 2019-05-02 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain u
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 12 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.90 Å |
| 6OUO RF2 accommodated state bound 70S complex at long incubation time Deposited 2019-05-05 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain u
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 12 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.70 Å |
| 6VU3 Cryo-EM structure of Escherichia coli transcription-translation complex A (TTC-A) containing mRNA with a 12 nt long spacer Deposited 2020-02-14 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6VYQ Escherichia coli transcription-translation complex A1 (TTC-A1) containing an 15 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6VYR Escherichia coli transcription-translation complex A1 (TTC-A1) containing an 18 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6VYS Escherichia coli transcription-translation complex A1 (TTC-A1) containing a 21 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6VYT Escherichia coli transcription-translation complex A2 (TTC-A2) containing a 15 nt long mRNA spacer, NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 55 PDB declaration: 64-meric（64） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 14.00 Å |
| 6VYU Escherichia coli transcription-translation complex C2 (TTC-C2) containing a 27 nt long mRNA spacer Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.00 Å |
| 6VYW Escherichia coli transcription-translation complex C3 (TTC-C3) containing mRNA with a 27 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.00 Å |
| 6VYX Escherichia coli transcription-translation complex C4 (TTC-C4) containing mRNA with a 21 nt long spacer, transcription factor NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.90 Å |
| 6VYY Escherichia coli transcription-translation complex C5 (TTC-C5) containing mRNA with a 21 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.90 Å |
| 6VYZ Escherichia coli transcription-translation complex C6 (TTC-C6) containing mRNA with a 21 nt long spacer, NusA, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.90 Å |
| 6VZ2 Escherichia coli transcription-translation complex D1 (TTC-D1) containing mRNA with a 27 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 10.00 Å |
| 6VZ3 Escherichia coli transcription-translation complex D2 (TTC-D2) containing mRNA with a 27 nt long spacer Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.90 Å |
| 6VZ5 Escherichia coli transcription-translation complex D3 (TTC-D3) containing mRNA with a 21 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.90 Å |
| 6VZ7 Escherichia coli transcription-translation complex C1 (TTC-C1) containing a 27 nt long mRNA spacer, NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.00 Å |
| 6VZJ Escherichia coli transcription-translation complex A1 (TTC-A1) containing mRNA with a 15 nt long spacer, fMet-tRNAs at E-site and P-site, and lacking transcription factor NusG Deposited 2020-02-28 | Assembly 1 Other combination Heteromer；Protein × 57 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 6WD0 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure I-A) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 6WD1 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure I-B) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 6WD2 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure II-A) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6WD3 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure II-B1) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6WD4 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure II-B2) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6WD5 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure II-C1) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6WD7 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure II-D) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6WD8 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure III-A) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6WD9 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure III-B) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6WDA Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure III-C) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6X6T Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B1 (TTC-B1) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-05-29 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6X7F Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B2 (TTC-B2) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-05-29 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6X7K Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-05-30 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6X9Q Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 27 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-06-03 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å |
| 6XDQ Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 30 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-06-11 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6XDR Escherichia coli transcription-translation complex B (TTC-B) containing an 27 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-11 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.70 Å |
| 6XGF Escherichia coli transcription-translation complex B (TTC-B) containing an 30 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-17 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.00 Å |
| 6XII Escherichia coli transcription-translation complex B (TTC-B) containing an 24 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-20 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.00 Å |
| 6XIJ Escherichia coli transcription-translation complex A (TTC-A) containing an 24 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-20 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain U
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.00 Å |
| 7K00 Structure of the Bacterial Ribosome at 2 Angstrom Resolution Deposited 2020-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain P
1–82（82 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 309 SPD SPERMIDINE × 16 SPM SPERMINE × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.98 Å |
| 7M5D Cryo-EM structure of a non-rotated E.coli 70S ribosome in complex with RF3-GTP, RF1 and P-tRNA (state I) Deposited 2021-03-23 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain u
1–82（82 aa）
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 7S1G wild-type Escherichia coli stalled ribosome with antibiotic linezolid Deposited 2021-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain x
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 196 ZLD N-{[(5S)-3-(3-fluoro-4-morpholin-4-ylphenyl)-2-oxo-1,3-oxazolidin-5-yl]methyl}acetamide × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.48 Å |
| 7S1H Wild-type Escherichia coli ribosome with antibiotic linezolid Deposited 2021-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain x
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 206 ZLD N-{[(5S)-3-(3-fluoro-4-morpholin-4-ylphenyl)-2-oxo-1,3-oxazolidin-5-yl]methyl}acetamide × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.35 Å |
| 7S1I Wild-type Escherichia coli stalled ribosome with antibiotic radezolid Deposited 2021-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain x
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 209 RD8 Radezolid × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.48 Å |
| 7S1J Wild-type Escherichia coli ribosome with antibiotic radezolid Deposited 2021-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain x
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 199 RD8 Radezolid × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.47 Å |
| 7S1K Cfr-modified Escherichia coli stalled ribosome with antibiotic radezolid Deposited 2021-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain x
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 207 RD8 Radezolid × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.42 Å |
| 7SA4 Damaged 70S ribosome with PrfH bound Deposited 2021-09-22 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain u
1–82（82 aa）
|
Not recorded | MG MAGNESIUM ION × 439 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.55 Å |
| 7TOS E. coli 70S ribosomes bound with the ALS/FTD-associated dipeptide repeat protein PR20 Deposited 2022-01-24 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain S16
1–82（82 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 7UG7 70S ribosome complex in an intermediate state of translocation bound to EF-G(GDP) stalled by Argyrin B Deposited 2022-03-24 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain SP
1–82（82 aa）
|
Not recorded | PUT 1,4-DIAMINOBUTANE × 11 MG MAGNESIUM ION × 404 SPD SPERMIDINE × 2 ZN ZINC ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.58 Å |