Current Protein Identity:D7Z9G0 New Search
Main Difference Dimensions in This Set
Different assembly state Different ligand/ion Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
5U9F 3.2 A cryo-EM ArfA-RF2 ribosome rescue complex (Structure II) Deposited 2016-12-16 Assembly 1 Protein–RNA Heteromer;Protein × 54 PDB declaration: 60-meric(60) Consistent with all polymers
Chain 21 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 360 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
5U9G 3.2 A cryo-EM ArfA-RF2 ribosome rescue complex (Structure I) Deposited 2016-12-16 Assembly 1 Protein–RNA Heteromer;Protein × 54 PDB declaration: 60-meric(60) Consistent with all polymers
Chain 21 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 348 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6O9J 70S Elongation Competent Ribosome Deposited 2019-03-14 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 53-meric(53) Consistent with all polymers
Chain S 1–110(110 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6O9K 70S initiation complex Deposited 2019-03-14 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain S 1–110(110 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
6OGF 70S termination complex with RF2 bound to the UGA codon. Partially rotated ribosome with RF2 bound (Structure III). Deposited 2019-04-02 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain s 1–110(110 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6OGG 70S termination complex with RF2 bound to the UGA codon. Rotated ribosome with RF2 bound (Structure IV). Deposited 2019-04-02 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain s 1–110(110 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
6OGI 70S termination complex with RF2 bound to the UAG codon. Rotated ribosome conformation (Structure V) Deposited 2019-04-02 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 54-meric(54) Consistent with all polymers
Chain s 1–110(110 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6OM6 Structure of trans-translation inhibitor bound to E. coli 70S ribosome with P site tRNA Deposited 2019-04-18 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 54-meric(54) Consistent with all polymers
Chain S 1–110(110 aa)
Not recorded KKL 4-amino-N-[5-(4-ethynylphenyl)-1,3,4-oxadiazol-2-yl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE;Blot time: 3.5 seconds
Resolution 3.10 Å
6ORE Release complex 70S Deposited 2019-04-30 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain S 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 436 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
6ORL RF1 pre-accommodated 70S complex at 24 ms Deposited 2019-04-30 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain S 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 295 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
6OSK RF1 accommodated 70S complex at 60 ms Deposited 2019-05-01 Assembly 1 Protein–RNA Heteromer;Protein × 51 PDB declaration: 56-meric(56) Consistent with all polymers
Chain S 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 307 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
6OSQ RF1 accommodated state bound Release complex 70S at long incubation time point Deposited 2019-05-02 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain S 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 436 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
6OST RF2 pre-accommodated state bound Release complex 70S at 24ms Deposited 2019-05-02 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain S 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 12 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
6OT3 RF2 accommodated state bound Release complex 70S at 24 ms Deposited 2019-05-02 Assembly 1 Protein–RNA Heteromer;Protein × 51 PDB declaration: 56-meric(56) Consistent with all polymers
Chain S 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 12 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å
6OUO RF2 accommodated state bound 70S complex at long incubation time Deposited 2019-05-05 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain S 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 12 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.70 Å
6PJ6 High resolution cryo-EM structure of E.coli 50S Deposited 2019-06-27 Assembly 1 Protein–RNA Heteromer;Protein × 29 PDB declaration: 31-meric(31) Consistent with all polymers
Chain a 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 185 NA SODIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5;Buffer A
cryo-EM vitrification conditions Cryogen ETHANE;Blot Force 5 Blot Time 10sec Hum 95% Temperature 10C Waiting time 1 min
Resolution 2.20 Å
6U48 E. coli 50S with phazolicin (PHZ) bound in exit tunnel Deposited 2019-08-23 Assembly 1 Protein–RNA Heteromer;Protein × 30 PDB declaration: 32-meric(32) Consistent with all polymers
Chain CT 1–110(110 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.87 Å
6VU3 Cryo-EM structure of Escherichia coli transcription-translation complex A (TTC-A) containing mRNA with a 12 nt long spacer Deposited 2020-02-14 Assembly 1 Other combination Heteromer;Protein × 58 PDB declaration: 66-meric(66) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6VYQ Escherichia coli transcription-translation complex A1 (TTC-A1) containing an 15 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 58 PDB declaration: 66-meric(66) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6VYR Escherichia coli transcription-translation complex A1 (TTC-A1) containing an 18 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 58 PDB declaration: 66-meric(66) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6VYS Escherichia coli transcription-translation complex A1 (TTC-A1) containing a 21 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 58 PDB declaration: 66-meric(66) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6VYT Escherichia coli transcription-translation complex A2 (TTC-A2) containing a 15 nt long mRNA spacer, NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 55 PDB declaration: 64-meric(64) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 14.00 Å
6VYU Escherichia coli transcription-translation complex C2 (TTC-C2) containing a 27 nt long mRNA spacer Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 56 PDB declaration: 65-meric(65) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.00 Å
6VYW Escherichia coli transcription-translation complex C3 (TTC-C3) containing mRNA with a 27 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 56 PDB declaration: 65-meric(65) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.00 Å
6VYX Escherichia coli transcription-translation complex C4 (TTC-C4) containing mRNA with a 21 nt long spacer, transcription factor NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 56 PDB declaration: 65-meric(65) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.90 Å
6VYY Escherichia coli transcription-translation complex C5 (TTC-C5) containing mRNA with a 21 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 56 PDB declaration: 65-meric(65) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.90 Å
6VYZ Escherichia coli transcription-translation complex C6 (TTC-C6) containing mRNA with a 21 nt long spacer, NusA, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 56 PDB declaration: 65-meric(65) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.90 Å
6VZ2 Escherichia coli transcription-translation complex D1 (TTC-D1) containing mRNA with a 27 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 56 PDB declaration: 65-meric(65) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 10.00 Å
6VZ3 Escherichia coli transcription-translation complex D2 (TTC-D2) containing mRNA with a 27 nt long spacer Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 56 PDB declaration: 65-meric(65) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.90 Å
6VZ5 Escherichia coli transcription-translation complex D3 (TTC-D3) containing mRNA with a 21 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 56 PDB declaration: 65-meric(65) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.90 Å
6VZ7 Escherichia coli transcription-translation complex C1 (TTC-C1) containing a 27 nt long mRNA spacer, NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-02-27 Assembly 1 Other combination Heteromer;Protein × 56 PDB declaration: 65-meric(65) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.00 Å
6VZJ Escherichia coli transcription-translation complex A1 (TTC-A1) containing mRNA with a 15 nt long spacer, fMet-tRNAs at E-site and P-site, and lacking transcription factor NusG Deposited 2020-02-28 Assembly 1 Other combination Heteromer;Protein × 57 PDB declaration: 65-meric(65) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
6WD0 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure I-A) Deposited 2020-03-31 Assembly 1 Protein–RNA Heteromer;Protein × 51 PDB declaration: 57-meric(57) Consistent with all polymers
Chain s 1–110(110 aa)
Not recorded FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6WD1 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure I-B) Deposited 2020-03-31 Assembly 1 Protein–RNA Heteromer;Protein × 51 PDB declaration: 56-meric(56) Consistent with all polymers
Chain s 1–110(110 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6WD2 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure II-A) Deposited 2020-03-31 Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 59-meric(59) Consistent with all polymers
Chain s 1–110(110 aa)
Not recorded FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6WD3 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure II-B1) Deposited 2020-03-31 Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 59-meric(59) Consistent with all polymers
Chain s 1–110(110 aa)
Not recorded FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6WD4 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure II-B2) Deposited 2020-03-31 Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 59-meric(59) Consistent with all polymers
Chain s 1–110(110 aa)
Not recorded FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6WD5 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure II-C1) Deposited 2020-03-31 Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 59-meric(59) Consistent with all polymers
Chain s 1–110(110 aa)
Not recorded FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6WD7 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure II-D) Deposited 2020-03-31 Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 59-meric(59) Consistent with all polymers
Chain s 1–110(110 aa)
Not recorded FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6WD8 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure III-A) Deposited 2020-03-31 Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 59-meric(59) Consistent with all polymers
Chain s 1–110(110 aa)
Not recorded FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6WD9 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure III-B) Deposited 2020-03-31 Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 59-meric(59) Consistent with all polymers
Chain s 1–110(110 aa)
Not recorded FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6WDA Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure III-C) Deposited 2020-03-31 Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 59-meric(59) Consistent with all polymers
Chain s 1–110(110 aa)
Not recorded FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6X6T Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B1 (TTC-B1) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-05-29 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6X7F Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B2 (TTC-B2) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-05-29 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
6X7K Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-05-30 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6X9Q Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 27 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-06-03 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.80 Å
6XDQ Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 30 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-06-11 Assembly 1 Other combination Heteromer;Protein × 60 PDB declaration: 68-meric(68) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6XDR Escherichia coli transcription-translation complex B (TTC-B) containing an 27 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-11 Assembly 1 Other combination Heteromer;Protein × 59 PDB declaration: 67-meric(67) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.70 Å
6XGF Escherichia coli transcription-translation complex B (TTC-B) containing an 30 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-17 Assembly 1 Other combination Heteromer;Protein × 59 PDB declaration: 67-meric(67) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
6XII Escherichia coli transcription-translation complex B (TTC-B) containing an 24 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-20 Assembly 1 Other combination Heteromer;Protein × 59 PDB declaration: 67-meric(67) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.00 Å
6XIJ Escherichia coli transcription-translation complex A (TTC-A) containing an 24 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-20 Assembly 1 Other combination Heteromer;Protein × 58 PDB declaration: 66-meric(66) Consistent with all polymers
Chain 1 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.00 Å
7K00 Structure of the Bacterial Ribosome at 2 Angstrom Resolution Deposited 2020-09-02 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 56-meric(56) Consistent with all polymers
Chain r 1–110(110 aa)
Not recorded PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 309 SPD SPERMIDINE × 16 SPM SPERMINE × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 1.98 Å
7LVK Cfr-modified 50S subunit from Escherichia coli Deposited 2021-02-25 Assembly 1 Protein–RNA Heteromer;Protein × 28 PDB declaration: 30-meric(30) Consistent with all polymers
Chain a 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 179 NA SODIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5;Buffer A
cryo-EM vitrification conditions Cryogen ETHANE;Blot Force 5 Blot Time 10sec Hum 95% Temperature 10C Waiting time 1 min
Resolution 2.20 Å
7M5D Cryo-EM structure of a non-rotated E.coli 70S ribosome in complex with RF3-GTP, RF1 and P-tRNA (state I) Deposited 2021-03-23 Assembly 1 Protein–RNA Heteromer;Protein × 54 PDB declaration: 59-meric(59) Consistent with all polymers
Chain S 1–110(110 aa)
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7S1G wild-type Escherichia coli stalled ribosome with antibiotic linezolid Deposited 2021-09-02 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers
Chain a 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 196 ZLD N-{[(5S)-3-(3-fluoro-4-morpholin-4-ylphenyl)-2-oxo-1,3-oxazolidin-5-yl]methyl}acetamide × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.48 Å
7S1H Wild-type Escherichia coli ribosome with antibiotic linezolid Deposited 2021-09-02 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 52-meric(52) Consistent with all polymers
Chain a 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 206 ZLD N-{[(5S)-3-(3-fluoro-4-morpholin-4-ylphenyl)-2-oxo-1,3-oxazolidin-5-yl]methyl}acetamide × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.35 Å
7SA4 Damaged 70S ribosome with PrfH bound Deposited 2021-09-22 Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 58-meric(58) Consistent with all polymers
Chain S 1–110(110 aa)
Not recorded MG MAGNESIUM ION × 439 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.55 Å