当前蛋白身份:G1SG72
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差异标签只比较当前检索结果;所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly;同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon 提交 2015-06-10 | Assembly 1 蛋白–RNA 异源复合物;蛋白 × 80 PDB 声明:87-meric(87) 与全部聚合物一致 |
链 jj
4–597(594 aa)
|
未记录 | MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT;pH 7.4;50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM玻璃化条件
After 30 second wait time, blot for 3 seconds before plunging;冷冻剂 ETHANE;After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
|
分辨率 3.65 Å |
| 3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon 提交 2015-06-10 | Assembly 1 蛋白–RNA 异源复合物;蛋白 × 80 PDB 声明:87-meric(87) 与全部聚合物一致 |
链 jj
4–597(594 aa)
|
未记录 | MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT;pH 7.4;50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM玻璃化条件
After 30 second wait time, blot for 3 seconds before plunging;冷冻剂 ETHANE;After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
|
分辨率 3.45 Å |
| 3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon 提交 2015-06-10 | Assembly 1 蛋白–RNA 异源复合物;蛋白 × 80 PDB 声明:87-meric(87) 与全部聚合物一致 |
链 jj
4–597(594 aa)
|
未记录 | MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT;pH 7.4;50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM玻璃化条件
After 30 second wait time, blot for 3 seconds before plunging;冷冻剂 ETHANE;After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
|
分辨率 3.65 Å |
| 5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. 提交 2016-10-02 | Assembly 1 蛋白–RNA 异源复合物;蛋白 × 80 PDB 声明:87-meric(87) 与全部聚合物一致 |
链 jj
1–599(599 aa)
|
未记录 | MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE;3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
|
分辨率 3.35 Å |
| 6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon 提交 2018-08-14 | Assembly 1 蛋白–RNA 异源复合物;蛋白 × 81 PDB 声明:87-meric(87) 与全部聚合物一致 |
链 k1
1–599(599 aa)
|
未记录 | MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.90 Å |
| 6HCM Structure of the rabbit collided di-ribosome (stalled monosome) 提交 2018-08-15 | Assembly 1 蛋白–RNA 异源复合物;蛋白 × 81 PDB 声明:87-meric(87) 与全部聚合物一致 |
链 k1
1–599(599 aa)
|
未记录 | MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 6.80 Å |
| 7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site 提交 2021-04-14 | Assembly 1 蛋白–RNA 异源复合物;蛋白 × 81 PDB 声明:88-meric(88) 与全部聚合物一致 |
链 Bz
1–599(599 aa)
|
未记录 | SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE-PROPANE
|
分辨率 2.90 Å |
| 8P03 48S late-stage initiation complex with m6A mRNA 提交 2023-05-09 | Assembly 1 蛋白–RNA 异源复合物;蛋白 × 37 PDB 声明:40-meric(40) 与全部聚合物一致 |
链 k
4–598(595 aa)
|
未记录 | MG MAGNESIUM ION × 178 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.04 Å |
| 8P09 48S late-stage initiation complex with non methylated mRNA 提交 2023-05-09 | Assembly 1 蛋白–RNA 异源复合物;蛋白 × 37 PDB 声明:40-meric(40) 与全部聚合物一致 |
链 k
4–598(595 aa)
|
未记录 | MG MAGNESIUM ION × 230 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.30 Å |
| 8SCB Terminating ribosome with SRI-41315 提交 2023-04-05 | Assembly 1 蛋白–RNA 异源复合物;蛋白 × 80 PDB 声明:87-meric(87) 与全部聚合物一致 |
链 jj
1–599(599 aa)
|
未记录 | MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.50 Å |
| 9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA 提交 2024-10-25 | Assembly 1 蛋白–RNA 异源复合物;蛋白 × 39 PDB 声明:42-meric(42) 与全部聚合物一致 |
链 k
1–599(599 aa)
|
未记录 | SF4 IRON/SULFUR CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4;10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM玻璃化条件
冷冻剂 ETHANE;The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
|
分辨率 2.90 Å |
| 9Q7Q ABCE1-eRF1-RNC-AMD1C 提交 2025-08-25 | Assembly 1 蛋白–RNA 异源复合物;蛋白 × 80 PDB 声明:86-meric(86) 与全部聚合物一致 |
链 AB
1–599(599 aa)
|
未记录 | MG MAGNESIUM ION × 291 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.86 Å |
| 9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence 提交 2025-06-10 | Assembly 1 蛋白–RNA 异源复合物;蛋白 × 81 PDB 声明:88-meric(88) 与全部聚合物一致 |
链 q2
1–599(599 aa)
|
未记录 | MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.65 Å |