当前蛋白身份:G1TUT9 重新检索
本组结构的主要差异维度
构建体不同 组装状态不同 配体/离子不同 实验环境不同 结构质量指标不同

差异标签只比较当前检索结果;所有PDB和assembly原始记录仍分别保留。

相关结构差异明细

一行代表一个 PDB 条目中的一个 biological assembly;同一蛋白的多个单体会分别列出。

PDB 条目 Assembly / 聚集状态 构建体 突变与修饰 配体、离子与非聚合物 实验方法 实验环境 结构质量
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon 提交 2015-06-10 Assembly 1 蛋白–RNA 异源复合物;蛋白 × 80 PDB 声明:87-meric(87) 与全部聚合物一致
链 CC 39–256(218 aa)
未记录 MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM缓冲液 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT;pH 7.4;50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM玻璃化条件 After 30 second wait time, blot for 3 seconds before plunging;冷冻剂 ETHANE;After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
分辨率 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon 提交 2015-06-10 Assembly 1 蛋白–RNA 异源复合物;蛋白 × 80 PDB 声明:87-meric(87) 与全部聚合物一致
链 CC 39–256(218 aa)
未记录 MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM缓冲液 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT;pH 7.4;50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM玻璃化条件 After 30 second wait time, blot for 3 seconds before plunging;冷冻剂 ETHANE;After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
分辨率 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon 提交 2015-06-10 Assembly 1 蛋白–RNA 异源复合物;蛋白 × 80 PDB 声明:87-meric(87) 与全部聚合物一致
链 CC 39–256(218 aa)
未记录 MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM缓冲液 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT;pH 7.4;50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM玻璃化条件 After 30 second wait time, blot for 3 seconds before plunging;冷冻剂 ETHANE;After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
分辨率 3.65 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) 提交 2021-11-25 Assembly 1 蛋白–RNA 异源复合物;蛋白 × 34 PDB 声明:36-meric(36) 与全部聚合物一致
链 D 35–255(221 aa)
未记录 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5
cryo-EM玻璃化条件 冷冻剂 ETHANE-PROPANE;4 second blot time, force 3
分辨率 4.30 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) 提交 2021-11-25 Assembly 1 蛋白–RNA 异源复合物;蛋白 × 34 PDB 声明:36-meric(36) 与全部聚合物一致
链 D 35–255(221 aa)
未记录 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5
cryo-EM玻璃化条件 冷冻剂 ETHANE-PROPANE;4 second blot time, force 3
分辨率 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) 提交 2021-11-25 Assembly 1 蛋白–RNA 异源复合物;蛋白 × 34 PDB 声明:36-meric(36) 与全部聚合物一致
链 D 35–255(221 aa)
未记录 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5
cryo-EM玻璃化条件 冷冻剂 ETHANE-PROPANE;4 second blot time, force 3
分辨率 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) 提交 2021-11-25 Assembly 1 蛋白–RNA 异源复合物;蛋白 × 36 PDB 声明:39-meric(39) 与全部聚合物一致
链 D 35–255(221 aa)
未记录 ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5
cryo-EM玻璃化条件 冷冻剂 ETHANE-PROPANE;4 second blot time, force 3
分辨率 3.90 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR 提交 2022-01-24 Assembly 1 蛋白–RNA 异源复合物;蛋白 × 78 PDB 声明:84-meric(84) 与全部聚合物一致
链 AS02 35–255(221 aa)
未记录 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 提交 2022-01-24 Assembly 1 蛋白–RNA 异源复合物;蛋白 × 79 PDB 声明:86-meric(86) 与全部聚合物一致
链 AS02 35–255(221 aa)
未记录 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine 提交 2022-03-16 Assembly 1 蛋白–RNA 异源复合物;蛋白 × 73 PDB 声明:79-meric(79) 与全部聚合物一致
链 CC 35–255(221 aa)
未记录 MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine 提交 2022-03-16 Assembly 1 蛋白–RNA 异源复合物;蛋白 × 73 PDB 声明:79-meric(79) 与全部聚合物一致
链 CC 35–255(221 aa)
未记录 MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 2.80 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome 提交 2022-11-29 Assembly 1 信息不足 异源复合物;蛋白 × 86 PDB 声明:92-meric(92) 与全部聚合物一致
链 Ab 20–270(251 aa)
未记录 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5
cryo-EM玻璃化条件 冷冻剂 ETHANE-PROPANE;3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
分辨率 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA 提交 2023-05-09 Assembly 1 蛋白–RNA 异源复合物;蛋白 × 37 PDB 声明:40-meric(40) 与全部聚合物一致
链 E 1–270(270 aa)
未记录 MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.4
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA 提交 2023-05-09 Assembly 1 蛋白–RNA 异源复合物;蛋白 × 37 PDB 声明:40-meric(40) 与全部聚合物一致
链 E 1–270(270 aa)
未记录 MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.4
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 3.30 Å