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Protein Structures and Oligomeric States

Enter a protein name, UniProt accession or PDB ID. Name searches ask you to confirm the protein identity before related structures are queried.

Current Protein Identity：G1U344 New Search
38PDB entries
38Biological assembly
38chain/assembly records
Main Difference Dimensions in This Set
Different construct Different mutation/modification Different assembly state Different ligand/ion Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric（86） Consistent with all polymers
Chain o 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric（87） Consistent with all polymers
Chain o 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric（85） Consistent with all polymers
Chain o 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric（87） Consistent with all polymers
Chain o 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric（87） Consistent with all polymers
Chain o 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric（87） Consistent with all polymers
Chain o 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers
Chain o 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric（87） Consistent with all polymers
Chain o 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric（81） Consistent with all polymers
Chain Ao 1–106（106 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric（81） Consistent with all polymers
Chain Ao 1–106（106 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric（81） Consistent with all polymers
Chain Ao 1–106（106 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric（82） Consistent with all polymers
Chain Ao 1–106（106 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers
Chain o2 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6T59 Structure of rabbit 80S ribosome translating beta-tubulin in complex with tetratricopeptide protein 5 and nascent chain-associated complex Deposited 2019-10-15 Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric（53） Consistent with all polymers
Chain o3 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 220 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.11 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric（81） Consistent with all polymers
Chain o 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers
Chain Bo 1–106（106 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers
Chain Bo 1–106（106 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric（88） Consistent with all polymers
Chain Bo 1–106（106 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric（87） Consistent with all polymers
Chain Bo 1–106（106 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric（83） Consistent with all polymers
Chain o 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7TM3 Structure of the rabbit 80S ribosome stalled on a 2-TMD Rhodopsin intermediate in complex with the multipass translocon Deposited 2022-01-19 Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 54-meric（54） Consistent with all polymers
Chain o 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 220 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；50 mM HEPES-KOH, pH 7.5, 200 mM KOAc, 5 mM Mg(OAc)2, 0.25% digitonin, 50 mM biotin
cryo-EM vitrification conditions Cryogen ETHANE；blot for 4 sec with Whatman filter papers at a blot force of -15 before plunging
Resolution 3.25 Å
7TUT Structure of the rabbit 80S ribosome stalled on a 4-TMD Rhodopsin intermediate in complex with the multipass translocon Deposited 2022-02-03 Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 56-meric（56） Consistent with all polymers
Chain o 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 220 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；50 mM HEPES-KOH, pH 7.5, 200 mM potassium acetate, 5 mM magnesium acetate, 0.25% digitonin, 50 mM biotin
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds with Whatman filter papers at a blot force of -15 before plunging.
Resolution 3.88 Å
8B5L Cryo-EM structure of ribosome-Sec61-TRAP (TRanslocon Associated Protein) translocon complex Deposited 2022-09-23 Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers
Chain o 2–104（103 aa）
Not recorded MG MAGNESIUM ION × 178 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
8B6C Cryo-EM structure of ribosome-Sec61 in complex with cyclotriazadisulfonamide derivative CK147 Deposited 2022-09-26 Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric（47） Consistent with all polymers
Chain o 2–104（103 aa）
Not recorded MG MAGNESIUM ION × 178 ZN ZINC ION × 5 OWI 4-[[9-(cyclohexylmethyl)-3-methylidene-5-(4-methylphenyl)sulfonyl-1,5,9-triazacyclododec-1-yl]sulfonyl]-~{N},~{N}-dimethyl-aniline × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.79 Å
8BPO Structure of rabbit 80S ribosome translating beta-tubulin in complex with tetratricopeptide protein 5 (TTC5) and S-phase Cyclin A Associated Protein residing in the ER (SCAPER) Deposited 2022-11-17 Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers
Chain n2 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 214 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric（92） Consistent with all polymers
Chain Bo 1–106（106 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric（88） Consistent with all polymers
Chain Bo 1–106（106 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8RJB Structure of the rabbit 80S ribosome stalled on a 2-TMD rhodopsin intermediate in complex with Sec61-RAMP4 Deposited 2023-12-20 Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 51-meric（51） Consistent with all polymers
Chain o 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.69 Å
8RJC Structure of the rabbit 80S ribosome stalled on a 2-TMD rhodopsin intermediate in complex with Sec61-TRAP, open conformation 1 Deposited 2023-12-20 Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 56-meric（56） Consistent with all polymers
Chain o 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RJD Structure of the rabbit 80S ribosome stalled on a 2-TMD rhodopsin intermediate in complex with Sec61-TRAP, open conformation 2 Deposited 2023-12-20 Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 56-meric（56） Consistent with all polymers
Chain o 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.79 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric（87） Consistent with all polymers
Chain o 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric（85） Consistent with all polymers
Chain o 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric（88） Consistent with all polymers
Chain NA 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric（88） Consistent with all polymers
Chain NA 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric（88） Consistent with all polymers
Chain NA 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric（88） Consistent with all polymers
Chain NA 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric（84） Consistent with all polymers
Chain NA 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric（86） Consistent with all polymers
Chain NA 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å

This page presents structural evidence recorded in PDB/mmCIF.“No recorded non-water small molecule” does not prove that the experimental sample was ligand-free; experimental conditions belong to the PDB experiment, while ligands and copy counts are shown for each biological assembly.