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Protein Structures and Oligomeric States

Enter a protein name, UniProt accession or PDB ID. Name searches ask you to confirm the protein identity before related structures are queried.

Current Protein Identity：G1U472 New Search
47PDB entries
47Biological assembly
47chain/assembly records
Main Difference Dimensions in This Set
Different construct Different mutation/modification Different assembly state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric（35） Consistent with all polymers
Chain O 1–151（151 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric（36） Consistent with all polymers
Chain O 1–151（151 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric（37） Consistent with all polymers
Chain O 1–151（151 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric（36） Consistent with all polymers
Chain P 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric（36） Consistent with all polymers
Chain P 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric（81） Consistent with all polymers
Chain P 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric（81） Consistent with all polymers
Chain P 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric（81） Consistent with all polymers
Chain P 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric（82） Consistent with all polymers
Chain P 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric（43） Consistent with all polymers
Chain P 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric（44） Consistent with all polymers
Chain P 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric（34） Consistent with all polymers
Chain X 1–168（168 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric（35） Consistent with all polymers
Chain X 1–168（168 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric（81） Consistent with all polymers
Chain OO 1–168（168 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric（36） Consistent with all polymers
Chain P 1–168（168 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric（36） Consistent with all polymers
Chain P 1–168（168 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric（36） Consistent with all polymers
Chain P 1–168（168 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric（36） Consistent with all polymers
Chain P 1–168（168 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric（36） Consistent with all polymers
Chain P 1–168（168 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric（36） Consistent with all polymers
Chain P 1–168（168 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric（36） Consistent with all polymers
Chain P 1–168（168 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric（36） Consistent with all polymers
Chain P 1–168（168 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric（36） Consistent with all polymers
Chain P 1–168（168 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric（37） Consistent with all polymers
Chain P 1–168（168 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric（37） Consistent with all polymers
Chain P 1–168（168 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric（39） Consistent with all polymers
Chain P 1–168（168 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric（39） Consistent with all polymers
Chain P 1–168（168 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric（37） Consistent with all polymers
Chain P 1–168（168 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric（37） Consistent with all polymers
Chain P 1–168（168 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric（39） Consistent with all polymers
Chain P 1–168（168 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric（39） Consistent with all polymers
Chain P 1–168（168 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric（39） Consistent with all polymers
Chain P 1–168（168 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric（84） Consistent with all polymers
Chain AS14 33–168（136 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers
Chain AS14 33–167（135 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric（79） Consistent with all polymers
Chain OO 33–168（136 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric（79） Consistent with all polymers
Chain OO 33–168（136 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric（92） Consistent with all polymers
Chain An 18–168（151 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric（88） Consistent with all polymers
Chain An 18–168（151 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric（87） Consistent with all polymers
Chain OO 1–168（168 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric（85） Consistent with all polymers
Chain OO 1–168（168 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric（88） Consistent with all polymers
Chain OB 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric（88） Consistent with all polymers
Chain OB 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric（88） Consistent with all polymers
Chain OB 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric（88） Consistent with all polymers
Chain OB 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric（84） Consistent with all polymers
Chain OB 1–168（168 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric（86） Consistent with all polymers
Chain OB 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric（83） Consistent with all polymers
Chain OO 33–168（136 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å

This page presents structural evidence recorded in PDB/mmCIF.“No recorded non-water small molecule” does not prove that the experimental sample was ligand-free; experimental conditions belong to the PDB experiment, while ligands and copy counts are shown for each biological assembly.