当前蛋白身份:O13033 重新检索
本组结构的主要差异维度
组装状态不同 配体/离子不同 实验环境不同 结构质量指标不同

差异标签只比较当前检索结果;所有PDB和assembly原始记录仍分别保留。

相关结构差异明细

一行代表一个 PDB 条目中的一个 biological assembly;同一蛋白的多个单体会分别列出。

PDB 条目 Assembly / 聚集状态 构建体 突变与修饰 配体、离子与非聚合物 实验方法 实验环境 结构质量
3JBW Cryo-electron microscopy structure of RAG Paired Complex (with NBD, no symmetry) 提交 2015-10-21 Assembly 1 蛋白–DNA 异源复合物;蛋白 × 4 PDB 声明:decameric(10) 与全部聚合物一致
链 A 271–1031(761 aa)
链 C 271–1031(761 aa)
未记录 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM缓冲液 Polymix buffer;pH 7.5;150 mM NaCl, 20 mM HEPES, 10 mM CaCl2, 1 mM TCEP
cryo-EM玻璃化条件 Blot for 2.5 seconds before plunging.;120 K;冷冻剂 ETHANE;Blot for 2.5 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3).
分辨率 4.60 Å R-free 0.464
3JBX Cryo-electron microscopy structure of RAG Signal End Complex (C2 symmetry) 提交 2015-10-22 Assembly 1 蛋白–DNA 异源复合物;蛋白 × 4 PDB 声明:dodecameric(12) 与全部聚合物一致
链 A 271–1031(761 aa)
链 C 271–1031(761 aa)
未记录 ZN ZINC ION × 2 MG MAGNESIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM缓冲液 Polymix buffer;pH 7.5;150 mM NaCl, 20 mM HEPES, 5 mM MgCl2, 1 mM TCEP
cryo-EM玻璃化条件 Blot for 2.5 seconds before plunging.;120 K;冷冻剂 ETHANE;Blot for 2.5 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3).
分辨率 3.40 Å R-free 0.415
3JBY Cryo-electron microscopy structure of RAG Paired Complex (C2 symmetry) 提交 2015-10-22 Assembly 1 蛋白–DNA 异源复合物;蛋白 × 4 PDB 声明:decameric(10) 与全部聚合物一致
链 A 271–1031(761 aa)
链 C 271–1031(761 aa)
未记录 ZN ZINC ION × 2 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM缓冲液 Polymix buffer;pH 7.5;150 mM NaCl, 20 mM HEPES, 10 mM CaCl2, 1 mM TCEP
cryo-EM玻璃化条件 Blot for 2.5 seconds before plunging.;120 K;冷冻剂 ETHANE;Blot for 2.5 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3).
分辨率 3.70 Å R-free 0.431
6DBI Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS nicked DNA intermediates 提交 2018-05-03 Assembly 1 信息不足 异源复合物;蛋白 × 4 PDB 声明:decameric(10) 与全部聚合物一致
链 A 271–1031(761 aa)
链 C 271–1031(761 aa)
未记录 ZN ZINC ION × 2 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 3.40 Å R-free 0.478
6DBJ Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS nicked DNA intermediates 提交 2018-05-03 Assembly 1 信息不足 异源复合物;蛋白 × 4 PDB 声明:decameric(10) 与全部聚合物一致
链 A 271–1031(761 aa)
链 C 271–1031(761 aa)
未记录 ZN ZINC ION × 2 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 3.00 Å R-free 0.384
6DBL Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs 提交 2018-05-03 Assembly 1 信息不足 异源复合物;蛋白 × 4 PDB 声明:octameric(8) 与全部聚合物一致
链 A 271–1031(761 aa)
链 C 271–1031(761 aa)
未记录 ZN ZINC ION × 2 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 5.00 Å R-free 0.410
6DBO Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs 提交 2018-05-03 Assembly 1 信息不足 异源复合物;蛋白 × 4 PDB 声明:octameric(8) 与全部聚合物一致
链 A 271–1031(761 aa)
链 C 271–1031(761 aa)
未记录 ZN ZINC ION × 2 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 4.40 Å R-free 0.452
6DBQ Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs 提交 2018-05-03 Assembly 1 信息不足 异源复合物;蛋白 × 4 PDB 声明:octameric(8) 与全部聚合物一致
链 A 271–1031(761 aa)
链 C 271–1031(761 aa)
未记录 ZN ZINC ION × 2 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 4.22 Å R-free 0.441
6DBR Cryo-EM structure of RAG in complex with one melted RSS and one unmelted RSS 提交 2018-05-03 Assembly 1 信息不足 异源复合物;蛋白 × 4 PDB 声明:octameric(8) 与全部聚合物一致
链 A 271–1031(761 aa)
链 C 271–1031(761 aa)
未记录 ZN ZINC ION × 2 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 4.00 Å R-free 0.417
6DBT Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs 提交 2018-05-03 Assembly 1 信息不足 异源复合物;蛋白 × 4 PDB 声明:octameric(8) 与全部聚合物一致
链 A 271–1031(761 aa)
链 C 271–1031(761 aa)
未记录 ZN ZINC ION × 2 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 4.30 Å R-free 0.443
6DBU Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs 提交 2018-05-03 Assembly 1 信息不足 异源复合物;蛋白 × 4 PDB 声明:octameric(8) 与全部聚合物一致
链 A 271–1031(761 aa)
链 C 271–1031(761 aa)
未记录 ZN ZINC ION × 2 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 3.90 Å R-free 0.451
6DBV Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs 提交 2018-05-03 Assembly 1 信息不足 异源复合物;蛋白 × 4 PDB 声明:octameric(8) 与全部聚合物一致
链 A 271–1031(761 aa)
链 C 271–1031(761 aa)
未记录 ZN ZINC ION × 2 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 4.29 Å R-free 0.433
6DBW Cryo-EM structure of RAG in complex with 12-RSS substrate DNA 提交 2018-05-03 Assembly 1 信息不足 异源复合物;蛋白 × 4 PDB 声明:hexameric(6) 与全部聚合物一致
链 A 271–1031(761 aa)
链 C 271–1031(761 aa)
未记录 ZN ZINC ION × 2 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 4.70 Å R-free 0.386
6DBX Cryo-EM structure of RAG in complex with 12-RSS substrate DNA 提交 2018-05-03 Assembly 1 信息不足 异源复合物;蛋白 × 4 PDB 声明:hexameric(6) 与全部聚合物一致
链 A 271–1031(761 aa)
链 C 271–1031(761 aa)
未记录 ZN ZINC ION × 2 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 4.20 Å R-free 0.421