Current Protein Identity:O13033
New Search
Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 3JBW Cryo-electron microscopy structure of RAG Paired Complex (with NBD, no symmetry) Deposited 2015-10-21 | Assembly 1 Protein–DNA Heteromer;Protein × 4 PDB declaration: decameric(10) Consistent with all polymers |
Chain A
271–1031(761 aa)
Chain C
271–1031(761 aa)
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
Polymix buffer;pH 7.5;150 mM NaCl, 20 mM HEPES, 10 mM CaCl2, 1 mM TCEP
cryo-EM vitrification conditions
Blot for 2.5 seconds before plunging.;120 K;Cryogen ETHANE;Blot for 2.5 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3).
|
Resolution 4.60 Å R-free 0.464 |
| 3JBX Cryo-electron microscopy structure of RAG Signal End Complex (C2 symmetry) Deposited 2015-10-22 | Assembly 1 Protein–DNA Heteromer;Protein × 4 PDB declaration: dodecameric(12) Consistent with all polymers |
Chain A
271–1031(761 aa)
Chain C
271–1031(761 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
Polymix buffer;pH 7.5;150 mM NaCl, 20 mM HEPES, 5 mM MgCl2, 1 mM TCEP
cryo-EM vitrification conditions
Blot for 2.5 seconds before plunging.;120 K;Cryogen ETHANE;Blot for 2.5 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3).
|
Resolution 3.40 Å R-free 0.415 |
| 3JBY Cryo-electron microscopy structure of RAG Paired Complex (C2 symmetry) Deposited 2015-10-22 | Assembly 1 Protein–DNA Heteromer;Protein × 4 PDB declaration: decameric(10) Consistent with all polymers |
Chain A
271–1031(761 aa)
Chain C
271–1031(761 aa)
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
Polymix buffer;pH 7.5;150 mM NaCl, 20 mM HEPES, 10 mM CaCl2, 1 mM TCEP
cryo-EM vitrification conditions
Blot for 2.5 seconds before plunging.;120 K;Cryogen ETHANE;Blot for 2.5 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3).
|
Resolution 3.70 Å R-free 0.431 |
| 6DBI Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS nicked DNA intermediates Deposited 2018-05-03 | Assembly 1 Insufficient information Heteromer;Protein × 4 PDB declaration: decameric(10) Consistent with all polymers |
Chain A
271–1031(761 aa)
Chain C
271–1031(761 aa)
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å R-free 0.478 |
| 6DBJ Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS nicked DNA intermediates Deposited 2018-05-03 | Assembly 1 Insufficient information Heteromer;Protein × 4 PDB declaration: decameric(10) Consistent with all polymers |
Chain A
271–1031(761 aa)
Chain C
271–1031(761 aa)
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å R-free 0.384 |
| 6DBL Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs Deposited 2018-05-03 | Assembly 1 Insufficient information Heteromer;Protein × 4 PDB declaration: octameric(8) Consistent with all polymers |
Chain A
271–1031(761 aa)
Chain C
271–1031(761 aa)
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.00 Å R-free 0.410 |
| 6DBO Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs Deposited 2018-05-03 | Assembly 1 Insufficient information Heteromer;Protein × 4 PDB declaration: octameric(8) Consistent with all polymers |
Chain A
271–1031(761 aa)
Chain C
271–1031(761 aa)
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.40 Å R-free 0.452 |
| 6DBQ Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs Deposited 2018-05-03 | Assembly 1 Insufficient information Heteromer;Protein × 4 PDB declaration: octameric(8) Consistent with all polymers |
Chain A
271–1031(761 aa)
Chain C
271–1031(761 aa)
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.22 Å R-free 0.441 |
| 6DBR Cryo-EM structure of RAG in complex with one melted RSS and one unmelted RSS Deposited 2018-05-03 | Assembly 1 Insufficient information Heteromer;Protein × 4 PDB declaration: octameric(8) Consistent with all polymers |
Chain A
271–1031(761 aa)
Chain C
271–1031(761 aa)
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å R-free 0.417 |
| 6DBT Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs Deposited 2018-05-03 | Assembly 1 Insufficient information Heteromer;Protein × 4 PDB declaration: octameric(8) Consistent with all polymers |
Chain A
271–1031(761 aa)
Chain C
271–1031(761 aa)
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å R-free 0.443 |
| 6DBU Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs Deposited 2018-05-03 | Assembly 1 Insufficient information Heteromer;Protein × 4 PDB declaration: octameric(8) Consistent with all polymers |
Chain A
271–1031(761 aa)
Chain C
271–1031(761 aa)
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å R-free 0.451 |
| 6DBV Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs Deposited 2018-05-03 | Assembly 1 Insufficient information Heteromer;Protein × 4 PDB declaration: octameric(8) Consistent with all polymers |
Chain A
271–1031(761 aa)
Chain C
271–1031(761 aa)
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.29 Å R-free 0.433 |
| 6DBW Cryo-EM structure of RAG in complex with 12-RSS substrate DNA Deposited 2018-05-03 | Assembly 1 Insufficient information Heteromer;Protein × 4 PDB declaration: hexameric(6) Consistent with all polymers |
Chain A
271–1031(761 aa)
Chain C
271–1031(761 aa)
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.70 Å R-free 0.386 |
| 6DBX Cryo-EM structure of RAG in complex with 12-RSS substrate DNA Deposited 2018-05-03 | Assembly 1 Insufficient information Heteromer;Protein × 4 PDB declaration: hexameric(6) Consistent with all polymers |
Chain A
271–1031(761 aa)
Chain C
271–1031(761 aa)
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;Solutions were made fresh from concentrated to avoid microbial contamination.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å R-free 0.421 |