Current Protein Identity：O35274
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2G5M Spinophilin PDZ domain Deposited 2006-02-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
493–602（110 aa）
Fragment：PDZ domain
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；298 K；Ionic strength (raw mmCIF value) 20 mM Na phosphate; 50 mM NaCl；Pressure ambient
NMR sample composition
1.8 mM PDZ domain, 15N samples; 20 mM Na phosphate buffer 50 mM NaCl, pH 6.5; 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition
1.8 mM PDZ domain, 13C/15N samples; 20 mM Na phosphate buffer 50 mM NaCl, pH 6.5; 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition
1.8 mM PDZ domain, 13C/15N samples; 20 mM Na phosphate buffer 50 mM NaCl, pH 6.5; 90% H2O, 10% D2O | 100% d20
|
Resolution not provided |
| 3EGG Crystal structure of a complex between Protein Phosphatase 1 alpha (PP1) and the PP1 binding and PDZ domains of Spinophilin Deposited 2008-09-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
417–583（167 aa）
Fragment：PP1 binding and PDZ domains
|
Not recorded | GOL GLYCEROL × 4 MN MANGANESE (II) ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；0.2M NaCl, 0.1M MES, 10% PEG 4000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.85 Å R-free 0.211 |
| 3EGG Crystal structure of a complex between Protein Phosphatase 1 alpha (PP1) and the PP1 binding and PDZ domains of Spinophilin Deposited 2008-09-10 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
417–583（167 aa）
Fragment：PP1 binding and PDZ domains
|
Not recorded | GOL GLYCEROL × 3 MN MANGANESE (II) ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；0.2M NaCl, 0.1M MES, 10% PEG 4000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.85 Å R-free 0.211 |
| 3EGH Crystal structure of a complex between Protein Phosphatase 1 alpha (PP1), the PP1 binding and PDZ domains of Spinophilin and the small natural molecular toxin Nodularin-R Deposited 2008-09-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain C
417–583（167 aa）
Fragment：PP1 binding and PDZ domains
Chain D
417–583（167 aa）
Fragment：PP1 binding and PDZ domains
|
Not recorded | GOL GLYCEROL × 4 MN MANGANESE (II) ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；277 K；0.1M MES, 15% PEG 550 MME, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.235 |
| 3EGH Crystal structure of a complex between Protein Phosphatase 1 alpha (PP1), the PP1 binding and PDZ domains of Spinophilin and the small natural molecular toxin Nodularin-R Deposited 2008-09-10 | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
417–583（167 aa）
Fragment：PP1 binding and PDZ domains
|
Not recorded | GOL GLYCEROL × 4 MN MANGANESE (II) ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；277 K；0.1M MES, 15% PEG 550 MME, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.235 |
| 3EGH Crystal structure of a complex between Protein Phosphatase 1 alpha (PP1), the PP1 binding and PDZ domains of Spinophilin and the small natural molecular toxin Nodularin-R Deposited 2008-09-10 | Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain D
417–583（167 aa）
Fragment：PP1 binding and PDZ domains
|
Not recorded | MN MANGANESE (II) ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；277 K；0.1M MES, 15% PEG 550 MME, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.235 |