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Protein Structures and Oligomeric States

Enter a protein name, UniProt accession or PDB ID. Name searches ask you to confirm the protein identity before related structures are queried.

Current Protein Identity：O67198 New Search
13PDB entries
24Biological assembly
105chain/assembly records
Main Difference Dimensions in This Set
Different construct Different mutation/modification Different assembly state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
1NY5 Crystal structure of sigm54 activator (AAA+ ATPase) in the inactive state Deposited 2003-02-11 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–387（387 aa） Fragment：Regulatory and Central domain
Chain B 1–387（387 aa） Fragment：Regulatory and Central domain
Not recorded MG MAGNESIUM ION × 1 PO4 PHOSPHATE ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 2 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；sodium/potasium phosphate, citric acid,imidazole,methanol,glycerol,, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.40 Å R-free 0.257
1NY6 Crystal structure of sigm54 activator (AAA+ ATPase) in the active state Deposited 2003-02-11 Assembly 1 Protein homooligomer Homooligomer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count
Chain A 122–387（266 aa） Fragment：residues 122-387
Chain B 122–387（266 aa） Fragment：residues 122-387
Chain C 122–387（266 aa） Fragment：residues 122-387
Chain D 122–387（266 aa） Fragment：residues 122-387
Chain E 122–387（266 aa） Fragment：residues 122-387
Chain F 122–387（266 aa） Fragment：residues 122-387
Chain G 122–387（266 aa） Fragment：residues 122-387
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；diammonium tartrate, PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 3.10 Å R-free 0.329
1NY6 Crystal structure of sigm54 activator (AAA+ ATPase) in the active state Deposited 2003-02-11 Assembly 2 Protein homooligomer Homooligomer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count
Chain H 122–387（266 aa） Fragment：residues 122-387
Chain I 122–387（266 aa） Fragment：residues 122-387
Chain J 122–387（266 aa） Fragment：residues 122-387
Chain K 122–387（266 aa） Fragment：residues 122-387
Chain L 122–387（266 aa） Fragment：residues 122-387
Chain M 122–387（266 aa） Fragment：residues 122-387
Chain N 122–387（266 aa） Fragment：residues 122-387
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；diammonium tartrate, PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 3.10 Å R-free 0.329
1ZY2 Crystal structure of the phosphorylated receiver domain of the transcription regulator NtrC1 from Aquifex aeolicus Deposited 2005-06-09 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–136（136 aa）
Chain B 1–136（136 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；310 K；PEG-3350, AMMONIUM CHLORIDE, GLYCEROL, CITRIC ACID, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 310K
Resolution 3.03 Å R-free 0.283
3M0E Crystal structure of the ATP-bound state of Walker B mutant of NtrC1 ATPase domain Deposited 2010-03-02 Assembly 1 Protein homooligomer Homooligomer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count
Chain A 122–387（266 aa） Fragment：ATP-ase domain
Chain B 122–387（266 aa） Fragment：ATP-ase domain
Chain C 122–387（266 aa） Fragment：ATP-ase domain
Chain D 122–387（266 aa） Fragment：ATP-ase domain
Chain E 122–387（266 aa） Fragment：ATP-ase domain
Chain F 122–387（266 aa） Fragment：ATP-ase domain
Chain G 122–387（266 aa） Fragment：ATP-ase domain
Mutation：E239A Mutation：E239A Mutation：E239A Mutation：E239A Mutation：E239A Mutation：E239A Mutation：E239A ATP ADENOSINE-5'-TRIPHOSPHATE × 7 MG MAGNESIUM ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；0.1 M SODIUM CITRATE, 0.01 M FECL3, 0-5% (V/V) JEFFAMINE M-600, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.63 Å R-free 0.241
4BT0 MuB is an AAAplus ATPase that forms helical filaments to control target selection for DNA transposition Deposited 2013-06-12 Assembly 1 Protein homooligomer Homooligomer；Protein × 14 PDB declaration: helical（14） Consistent with protein count
Chain A 312–384（73 aa） Fragment：AAAPLUS DOMAIN, RESIDUES 312-384
Chain B 137–309（173 aa） Fragment：AAAPLUS DOMAIN, RESIDUES 137-309
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 14 ELECTRON MICROSCOPY
cryo-EM buffer 30 MM TRISHCL PH 8.0, 0.3 M KCL, 5MM MGCL2, 1MM DTT, 1 MM ATP OR ATP-GAMMA-S；pH 8；30 MM TRISHCL PH 8.0, 0.3 M KCL, 5MM MGCL2, 1MM DTT, 1 MM ATP OR ATP-GAMMA-S
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 17.00 Å
4BT0 MuB is an AAAplus ATPase that forms helical filaments to control target selection for DNA transposition Deposited 2013-06-12 Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 312–384（73 aa） Fragment：AAAPLUS DOMAIN, RESIDUES 312-384
Chain B 137–309（173 aa） Fragment：AAAPLUS DOMAIN, RESIDUES 137-309
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 30 MM TRISHCL PH 8.0, 0.3 M KCL, 5MM MGCL2, 1MM DTT, 1 MM ATP OR ATP-GAMMA-S；pH 8；30 MM TRISHCL PH 8.0, 0.3 M KCL, 5MM MGCL2, 1MM DTT, 1 MM ATP OR ATP-GAMMA-S
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 17.00 Å
4BT0 MuB is an AAAplus ATPase that forms helical filaments to control target selection for DNA transposition Deposited 2013-06-12 Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 312–384（73 aa） Fragment：AAAPLUS DOMAIN, RESIDUES 312-384
Chain B 137–309（173 aa） Fragment：AAAPLUS DOMAIN, RESIDUES 137-309
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 30 MM TRISHCL PH 8.0, 0.3 M KCL, 5MM MGCL2, 1MM DTT, 1 MM ATP OR ATP-GAMMA-S；pH 8；30 MM TRISHCL PH 8.0, 0.3 M KCL, 5MM MGCL2, 1MM DTT, 1 MM ATP OR ATP-GAMMA-S
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 17.00 Å
4BT1 MuB is an AAAplus ATPase that forms helical filaments to control target selection for DNA transposition Deposited 2013-06-12 Assembly 1 Protein homooligomer Homooligomer；Protein × 14 PDB declaration: helical（14） Consistent with protein count
Chain A 312–384（73 aa） Fragment：AAAPLUS DOMAIN, RESIDUES 312-384
Chain B 137–309（173 aa） Fragment：AAAPLUS DOMAIN, RESIDUES 137-309
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 14 ELECTRON MICROSCOPY
cryo-EM buffer 30 MM TRISHCL PH 8.0, 0.3 M KCL, 5MM MGCL2, 1MM DTT, 1 MM ATP OR ATP-GAMMA-S；pH 8；30 MM TRISHCL PH 8.0, 0.3 M KCL, 5MM MGCL2, 1MM DTT, 1 MM ATP OR ATP-GAMMA-S
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 16.00 Å
4BT1 MuB is an AAAplus ATPase that forms helical filaments to control target selection for DNA transposition Deposited 2013-06-12 Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 312–384（73 aa） Fragment：AAAPLUS DOMAIN, RESIDUES 312-384
Chain B 137–309（173 aa） Fragment：AAAPLUS DOMAIN, RESIDUES 137-309
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 30 MM TRISHCL PH 8.0, 0.3 M KCL, 5MM MGCL2, 1MM DTT, 1 MM ATP OR ATP-GAMMA-S；pH 8；30 MM TRISHCL PH 8.0, 0.3 M KCL, 5MM MGCL2, 1MM DTT, 1 MM ATP OR ATP-GAMMA-S
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 16.00 Å
4BT1 MuB is an AAAplus ATPase that forms helical filaments to control target selection for DNA transposition Deposited 2013-06-12 Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 312–384（73 aa） Fragment：AAAPLUS DOMAIN, RESIDUES 312-384
Chain B 137–309（173 aa） Fragment：AAAPLUS DOMAIN, RESIDUES 137-309
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 30 MM TRISHCL PH 8.0, 0.3 M KCL, 5MM MGCL2, 1MM DTT, 1 MM ATP OR ATP-GAMMA-S；pH 8；30 MM TRISHCL PH 8.0, 0.3 M KCL, 5MM MGCL2, 1MM DTT, 1 MM ATP OR ATP-GAMMA-S
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 16.00 Å
4L4U Crystal structure of construct containing A. aeolicus NtrC1 receiver, central and DNA binding domains Deposited 2013-06-09 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–439（439 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.20 Å R-free 0.248
4L5E Crystal structure of A. aeolicus NtrC1 DNA binding domain Deposited 2013-06-10 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 393–438（46 aa） Fragment：unp residues 393-438
Not recorded SO4 SULFATE ION × 4 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.34 Å R-free 0.206
4LY6 Nucleotide-induced asymmetry within ATPase activator ring drives s54-RNAP interaction and ATP hydrolysis Deposited 2013-07-30 Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain A 121–387（267 aa） Fragment：UNP residues 121-387
Chain B 121–387（267 aa） Fragment：UNP residues 121-387
Chain C 121–387（267 aa） Fragment：UNP residues 121-387
Chain D 121–387（267 aa） Fragment：UNP residues 121-387
Chain E 121–387（267 aa） Fragment：UNP residues 121-387
Chain F 121–387（267 aa） Fragment：UNP residues 121-387
Not recorded 08T [[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium × 5 MG MAGNESIUM ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.9；277 K；20% ethylene glycol, pH 7.9, vapor diffusion, hanging drop, temperature 277K
Resolution 3.60 Å R-free 0.308
4LY6 Nucleotide-induced asymmetry within ATPase activator ring drives s54-RNAP interaction and ATP hydrolysis Deposited 2013-07-30 Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain G 121–387（267 aa） Fragment：UNP residues 121-387
Chain H 121–387（267 aa） Fragment：UNP residues 121-387
Chain I 121–387（267 aa） Fragment：UNP residues 121-387
Chain J 121–387（267 aa） Fragment：UNP residues 121-387
Chain K 121–387（267 aa） Fragment：UNP residues 121-387
Chain L 121–387（267 aa） Fragment：UNP residues 121-387
Not recorded 08T [[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium × 5 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.9；277 K；20% ethylene glycol, pH 7.9, vapor diffusion, hanging drop, temperature 277K
Resolution 3.60 Å R-free 0.308
4LY6 Nucleotide-induced asymmetry within ATPase activator ring drives s54-RNAP interaction and ATP hydrolysis Deposited 2013-07-30 Assembly 3 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain M 121–387（267 aa） Fragment：UNP residues 121-387
Chain N 121–387（267 aa） Fragment：UNP residues 121-387
Chain O 121–387（267 aa） Fragment：UNP residues 121-387
Chain P 121–387（267 aa） Fragment：UNP residues 121-387
Chain Q 121–387（267 aa） Fragment：UNP residues 121-387
Chain R 121–387（267 aa） Fragment：UNP residues 121-387
Not recorded 08T [[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium × 5 MG MAGNESIUM ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.9；277 K；20% ethylene glycol, pH 7.9, vapor diffusion, hanging drop, temperature 277K
Resolution 3.60 Å R-free 0.308
4LY6 Nucleotide-induced asymmetry within ATPase activator ring drives s54-RNAP interaction and ATP hydrolysis Deposited 2013-07-30 Assembly 4 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain S 121–387（267 aa） Fragment：UNP residues 121-387
Chain T 121–387（267 aa） Fragment：UNP residues 121-387
Chain U 121–387（267 aa） Fragment：UNP residues 121-387
Chain V 121–387（267 aa） Fragment：UNP residues 121-387
Chain W 121–387（267 aa） Fragment：UNP residues 121-387
Chain X 121–387（267 aa） Fragment：UNP residues 121-387
Not recorded 08T [[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium × 5 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.9；277 K；20% ethylene glycol, pH 7.9, vapor diffusion, hanging drop, temperature 277K
Resolution 3.60 Å R-free 0.308
4LZZ Nucleotide-induced asymmetry within atpase activator ring drives s54-RNAP interaction and ATP hydrolysis Deposited 2013-08-01 Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain A 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain B 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain C 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain D 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain E 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain F 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Not recorded 08T [[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.9；277 K；20% ethylene glycol, crystal soaked in fresh mother liquor prior to flash cooling, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 3.21 Å R-free 0.322
4LZZ Nucleotide-induced asymmetry within atpase activator ring drives s54-RNAP interaction and ATP hydrolysis Deposited 2013-08-01 Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain G 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain H 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain I 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain J 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain K 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain L 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Not recorded 08T [[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.9；277 K；20% ethylene glycol, crystal soaked in fresh mother liquor prior to flash cooling, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 3.21 Å R-free 0.322
4LZZ Nucleotide-induced asymmetry within atpase activator ring drives s54-RNAP interaction and ATP hydrolysis Deposited 2013-08-01 Assembly 3 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain M 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain N 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain O 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain P 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain Q 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain R 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Not recorded 08T [[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium × 6 MG MAGNESIUM ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.9；277 K；20% ethylene glycol, crystal soaked in fresh mother liquor prior to flash cooling, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 3.21 Å R-free 0.322
4LZZ Nucleotide-induced asymmetry within atpase activator ring drives s54-RNAP interaction and ATP hydrolysis Deposited 2013-08-01 Assembly 4 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain S 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain T 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain U 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain V 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain W 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Chain X 121–387（267 aa） Fragment：ATPase Domain (UNP residues 121-387)
Not recorded 08T [[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium × 6 MG MAGNESIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.9；277 K；20% ethylene glycol, crystal soaked in fresh mother liquor prior to flash cooling, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 3.21 Å R-free 0.322
9MSE de novo SigN RNA polymerase transcription initiation intermediate with pre-catalytic bEBP state (RPi1 open ring) Deposited 2025-01-09 Assembly 1 Protein–DNA Heteromer；Protein × 12 PDB declaration: 16-meric（16） Consistent with all polymers
Chain A 121–387（267 aa）
Chain B 121–387（267 aa）
Chain C 121–387（267 aa）
Chain D 121–387（267 aa）
Chain E 121–387（267 aa）
Chain F 121–387（267 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 3 POP PYROPHOSPHATE 2- × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；40 mM Tris-HCl, pH 8/RT, 200 mM KCl, 10 mM MgCl2, 1 mM DTT; fluorinated fos-choline-8 (FC8F) added to a final concentration of 1.5 mM during grid preparation
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
9MSF de novo SigN RNA polymerase transcription initiation intermediate with post-catalytic bEBP state (RPi1 closed ring) Deposited 2025-01-09 Assembly 1 Protein–DNA Heteromer；Protein × 12 PDB declaration: 16-meric（16） Consistent with all polymers
Chain A 121–387（267 aa）
Chain B 121–387（267 aa）
Chain C 121–387（267 aa）
Chain D 121–387（267 aa）
Chain E 121–387（267 aa）
Chain F 121–387（267 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 POP PYROPHOSPHATE 2- × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；40 mM Tris-HCl, pH 8/RT, 200 mM KCl, 10 mM MgCl2, 1 mM DTT; fluorinated fos-choline-8 (FC8F) added to a final concentration of 1.5 mM during grid preparation
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
9MSG De novo SigN RNA polymerase transcription initiation intermediate with bound SigN-RII Deposited 2025-01-09 Assembly 1 Protein–DNA Heteromer；Protein × 12 PDB declaration: tetradecameric（14） Consistent with all polymers
Chain A 121–387（267 aa）
Chain B 121–387（267 aa）
Chain C 121–387（267 aa）
Chain D 121–387（267 aa）
Chain E 121–387（267 aa）
Chain F 121–387（267 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 POP PYROPHOSPHATE 2- × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；40 mM Tris-HCl, pH 8/RT, 200 mM KCl, 10 mM MgCl2, 1 mM DTT; fluorinated fos-choline-8 (FC8F) added to a final concentration of 1.5 mM during grid preparation
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å

This page presents structural evidence recorded in PDB/mmCIF.“No recorded non-water small molecule” does not prove that the experimental sample was ligand-free; experimental conditions belong to the PDB experiment, while ligands and copy counts are shown for each biological assembly.